Hydrophobic Interactions in Bioseparation - Applications of Modified Green Fluorescent Protein
This thesis describes interactions that are salt-promoted and mainly based on the hydrophobicity of proteins. A series of variants of the green fluorescent protein (GFP) was first constructed by site-directed mutagenesis. Several tags with various properties were then genetically fused to the N-terminus of GFP. The protein variants constructed had small characteristic differences in hydrophobicity
