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The conflicts concerning the preservation or destruction of the deserted church of Mårup in Denmark is followed from the the moment in 1928, when the church became redundant, untill 2004. The protection of the church is in conflict with the protection of the eroding cliff, the church as a symbol in conflict with the church as a source material, and the local people in conflict with central authori

Total internal reflection fluorescence microscopy to study GLUT4 trafficking

Total internal reflection fluorescence (TIRF) microscopy is a powerful method that allows examination of plasma membrane close events in real time. The last decade, the method has successfully been used to explore GLUT4 translocation in adipocytes. Here, we describe the procedure for studying GLUT4 trafficking using TIRF microscopy in isolated primary adipocytes.