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Expression of double Vitreoscilla hemoglobin enhances growth and alters ribosome and tRNA levels in Escherichia coli

In several organisms, expression of a gene encoding dimeric hemoglobin (VHb) from the obligate aerobic bacterium Vatreoscilla stercoraria has been shown to increase microaerobic cell growth and enhance oxygen-dependent cell metabolism. In an attempt to further improve these effects of VHb, a gene encoding two vhb genes connected by a short linker of six base pairs was constructed and expressed in

Analysis of strongly interfering slow frequency-hopping systems

In this report slow frequency-hopping systems are analyzed with respect to the probability of successful transmission of packets, and with respect to the throughput. It is assumed that collisions result in a loss of all information contained in the colliding packets, irrespective of the distance between the units of the system. Units transmit packets of different types with a certain probability,

GUTI: a new antigen in the Cromer blood group system

BACKGROUND: The Cromer blood group system consists of seven high-incidence and three low-incidence antigens carried on decay-accelerating factor (DAF). This report describes the identification and characterization of a new Cromer high-incidence antigen, named GUTI. STUDY DESIGN AND METHODS: RT-PCR and sequence analysis were performed on cDNA prepared from a Chilean donor whose serum contained the

The influence of lithium on the antidiuretic effect of desmopressin.

The objective of this study was to investigate the graded influence from lithium on the antidiuretic effects of desmopressin. Eight healthy male subjects participated in this open, randomised cross-over study with two periods comprising 6 days each. For each subject, one of the study days (6th day) was preceded by a period of lithium treatment. On the study days the subjects were orally water load

Isomeric decays in neutron-rich W, Os and Pt nuclei

Relativistic energy fragmentation of a 1 GeV/nucleon Pb-208 beam was used to populate a wide range of nuclei on both sides of the valley of beta-stability. The structure of these nuclei was studied by the identification of discrete gamma-ray transitions depopulating isomeric states with lifetimes in the nano- to millisecond range. Highlights of the experiment include the first observation of gamma

Efficient first-order performance estimation for high-order adaptive optics systems

It is shown how first-order performance estimation of high-orderadaptive optics (AO) systems may be efficiently implemented in a hybridnumerical simulation by the use of 1) sparse matrix techniques forwavefront reconstruction, 2) undersampled pupil-plane turbulence-inducedaberrations, and 3) analytical models that compensate - in the limit ofinfinite exposure time - for the errors introduced by un

The effect of assembly tolerance on performance of a tape application task: A pilot study

Task characteristics such as visual and manual demands influence the postures employed and the muscular load during task execution. According to ergonomic experts at a car assembly plant, these demands are difficult to predict during product and production development and can easily be underestimated. One such task with combined visual and manual demands is the application of deco-tape to the door

Nonsense-mediated mRNA decay in barley mutants allows the cloning of mutated genes by a microarray approach.

We have previously described a microarray approach to identify and clone genes from mutants of higher organisms. In the method cDNA of two mutants with similar phenotype are competitively hybridized to DNA clones arrayed on a glass slide. Clones corresponding to an mRNA that is not expressed in one of the strains due to a mutation will be specifically highlighted in the hybridization, which provid

Estimating the Two-Dimensional Coherence Function

In this paper, we extend the one-dimensional Capon-based magnitude square coherence (MSC) spectral estimator, to form two-dimensional Capon- and APES-based MSC spectral estimators. The resulting estimators are found to yield significantly improved estimates as compared to the typical Welch-based estimator. Furthermore, we introduce a computationally efficient time-updating of the presented MSC est

Tracking ultrafast excited-state bond-twisting motion in solution close to the Franck-Condon point

Applying optimal control to photoinduced trans-cis isomerization in condensed phase, the dynamics of bond-twisting motion of 1,1'-diethyl-4,4'-cyanine in methanol and propanol is revealed. The shape of the optimized pulse resulting from minimization of the photoisomer formation can be directly related to the initial excited-state dynamics in close proximity to the Franck-Condon point. The solvent

Color constancy in visual scene perception

In RoboCup, all objects in the game are color coded to allow easy segmentation and identification. However, color vision is very sensitive to the exact color of the illumination which makes the identification task much harder than is often anticipated. Algorithms that work in the lab in fluorescent light may not function at all in an actual game where incandescent lights are used. This article is

Levels of C-14 in the terrestrial environment in the vicinity of two European nuclear power plants

Radiocarbon is produced in all types of nuclear reactors. Most of the C-14 released into the environment is in the form of gaseous emissions. Recent data on the C-14 concentration found in terrestrial samples taken in the vicinity of nuclear power plants in Romania and Lithuania are presented. We found increased C-14 levels in the surroundings of both power plants. At the Romanian power plant Cern

Thermodynamic Modelling of Electrolyte Solutions with Application to the Pulp and Paper Industry

Popular Abstract in Swedish Elektrolyter är ämnen som sönderdelas i ett lösningsmedel och därvid ger upphov till positivt och negativt laddade joner. De starka elektrostatiska krafter i lösningen som detta i sin tur orsakar är typiska för elektrolytlösningar. Redan vid små tillsatser av en elektrolyt till ett lösningsmedel kan lösningens egenskaper förändras kraftigt. Lösningar av elektrolyter speA thermodynamic model for electrolyte solutions has been derived for binary and multicomponent solutions up to very high molalities. The model was extensively tested on 163 binary aqueous electrolyte systems up to very high molalities, and the results show that the model represents mean ionic activity coefficients and osmotic coefficients very accurately. The model parameters vary significantly be