Isolation of plaice (Pleuronectes platessa) α1-microglobulin : Conservation of structure and chromophore
A cDNA coding for plaice (Pleuronectes platessa) α1-microglobulin (Leaver et al., 1994, Comp. Biochem. Physiol. 108B, 275-281) was expressed and purified from baculovirus-infected insect cells. Specific monoclonal antibodies were then prepared and used to isolate the protein from plaice liver and serum. Mature 28.5 kDa α1-microglobulin was found in both liver and serum. The protein consisted of an