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Engineering of central carbon metabolism in recombinant Saccharomyces cerevisiae for improved production of biopolymers

En viktig komponent i vårt moderna samhälle är plaster och användandet av detta material ökar. Plaster skapas av komponenter som extraheras från petroleum, ett icke-förnybart fossilt bränsle. För en hållbar framtid måste användandet av petroleum minska. Alternativ till petroleum-baserade plaster är bio-plaster. Bioraffinaderier använder idag mindre än 20 % av biomassan för etanolproduktion, överskBioplastics are a promising alternative for petroleum-based plastics that today is on high demand in our contemporary lifestyles. They are made from biopolymers such as polyhydroxyalkanoates (PHAs). Producing biopolymers from waste material feedstock using microbes increases profitability and environmental benefits as well as sustainability for biorefineries. Saccharomyces cerevisiae is a robust m

How Do Bumblebees And Honeybees Exploit Food Resources In Different Habitat Qualities?

Foragers increase their fitness by choosing the most profitable food source to forage on. Food resource is spatially distributed in a landscape context and, most importantly, in a patch scale. Therefore, they have to make daily decisions about where to forage based on their ecological information about the surrounding environment. There is a lack of descriptive studies on the relationship between

Urbanization and Income Inequality in China in the period 2001-2012

This thesis studies the relationship between urbanization and income inequality in China in the period 2001 to 2012. To analyze this question, firstly, it studies the urbanization development by looking of the urbanization ratio, growth rate of urban population and the share of agriculture in GDP. Secondly, it conducts the Gini coefficient of urban and rural sector respectively based on the data p

Device development for doping evaluation in nanowires using capacitance-voltage measurements

This thesis aims to show the prospect of capacitance measurements over nanowire arrays as an evaluation method of the p-doping level in weakly p-doped p-n nanowires. The premise of the method is that there will be a high yield of statistical data regarding the doping level of the nanowires. The majority of the work carried out and presented in this thesis is however aimed at the manufacturing of a

Analysis of inducible protein factor degrading apo-catalase in Enterococcus faecalis

Hur bryts apo-katalas ned i bakterien Enterococcus faecalis? Väteperoxid, H2O2, är ett kemiskt ämne som används som blekningsmedel för allt från papper till hår. Det används också i somliga bränslen och desinfektionsmedel. Celler producerar vanligtvis väteperoxid som en biprodukt av sin metabolism. I celler ställer väteperoxid till potentiella problem då ämnet kan orsaka bildning av ”fria radikalCatalase is a heme-containing homo-tetrameric enzyme that catalyzes the degradation of hydrogen peroxide. Apo-catalase is in the bacterium Enterococcus faecalis produced also in the absence of heme. In this work I have enriched and studied proteolytic activity causing apo-catalase degradation in stationary growth phase E. faecalis cells. The activity was enriched from soluble cell free extract of

Theoretical study of the potential to improve indoor comfort in Botswana using solar water heating, solar PV and PV/T

The possibility of improving indoor climate in residential buildings in Botswana was examined as the foundation of this Master’s Thesis. Initially articles were studied to gain understanding of previous research in the field as well as to gain understanding of local energy related conditions. Furthermore, a software called TRNSYS was thoroughly studied as this was found to be a powerful tool able

Soil drying-rewetting and its influence on ecosystem carbon fluxess

Torr jord ger ifrån sig mer koldioxid när det regnar När det regnar på torr jord bildas under en kort tid mycket mer koldioxid än vad jorden vanligtvis avger. Detta fenomen har varit känt sen 1950-talet och kallas enkelt för torkning och återfuktning. Även om vi vet att det sker så vet vi väldigt lite om varför det sker. Till en början trodde man att det hade att göra med en ökad aktivitet hos ma

Women and Maids : Perceptions of domestic workers, house work and class among young, progressive, middle-to-upper class women in Delhi

This thesis analyses ten indepth interviews on domestic workers, class and gender roles with ten young, politically inclined and often adamantly feminist women towards the upper end of the Indian class spectrum. It aims to deepen the understanding of employerworker relations and gendered domestic roles in contemporary Indian households. Previous literature on the middletoupper class employers of d

Home Ground Advantage : Foreign Internet Companies and Guanxi Utilisation in China

The leading internet companies display a dominance in world markets unparalleled in many industries. Despite this, China remains a hurdle no major internet company has been able to overcome. The role of government, culture and strategic decisions are all posited as potential explanatory factors. This paper concentrates on the significance of guanxi as an explanatory factor, linking its utilisation

Density distributions of juvenile fish in South Australia - Focusing on the influence of coastal habtitats on climate change-induced range shifts in fish

How will baby fish react to global warming? As most people have been aware of, global warming is just one of many consequences of climate change and increasing CO2-levels in the atmosphere. The oceans are experts at absorbing energy, which makes the ocean temperature rise. In many parts of the world, especially Australia, the sea surface temperatures (SSTs) are increasing rapidly today. This is e

Analyzing Post-Soviet Civil Society of Azerbaijan and its Contribution to Democracy

This master thesis seeks to convey an alternative approach for studying post-soviet civil society, with a particular focus on Azerbaijan. It rejects utilization of mainstream and overused liberal-democratic approach to civil society, which has been a predominant contextual framework for growing body of literature on civil society’s contribution to democracy in the post-communist space. By using Gr

Synthesis of novel PfDHODH inhibitors based on 4-aminocoumarin structures

Malaria is an infectious disease caused by the protozoan of the genus Plasmodium. It is a major problem in third-world countries, with hundreds of millions of infections and millions of fatalities annually. The extreme challenge in malaria management is the resistance of parasites to traditional chemotherapies like chloroquine and artemisinin. Plasmodium falciparum dihydroorotate dehydrogenase (Pf

Can Everyone Be Included? Analysing the public-private partnership between the Ukrainian government and the consultancy company “SKL-International” in development assistance

The aims of this study are two-fold. Firstly, the purpose is to discuss consultancy companies as actors in private-public partnerships (PPPs) in development assistance. Secondly, the study aims to explore the actions of consultancy companies in partnership with country-beneficiaries. The theoretical base of this study is grounded in debates about PPPs in development and theoretical accounts of pos

Heparin Binding Protein and the Glycocalyx in Sepsis

Sepsis is a severe and often lethal inflammatory response to an infection. During sepsis the cells and organs no longer function as they should because of the intense immune response. Some of the cell dysfunction may be because of an important “sugar layer” known as the glycocalyx. In this study, we determined whether an inflammatory protein, heparin binding protein (HBP), might contribute to the

Development of an exome pipeline for the identification of single nucleotide variants and indels from ovarian cancer tissue

Approximately a quarter of a million women are diagnosed with ovarian cancer annually worldwide. Identifying the genes involved may ultimately lead to the development of corrective and preventive measures to fight this type of cancer. Next Generation Sequencing is a technology that can reveal the genes central to cancer initiation and development, however the analysis of the data is problematic du

Code and Inference in metaphor comprehension: interpretation of metaphorical language in Dante's Inferno by Italians and Australians

This thesis investigates the interpretation of metaphorical expressions in Dante’s famous Inferno and how this may be motivated through the use of codes and inference. Based on Coseriu’s Integral Linguistics (Coseriu, 1985), metaphorical expressions can be divided depending on the kind of knowledge that is required to interpret them. The first level is the universal level where metaphors can be in

Recombination and degeneration of a neo-sex chromosome: a RAD-analysis of the great reed warbler, Acrocephalus arundinaceus

Sex chromosome evolution is a fascinating research topic because sex chromosomes provide a snapshot of evolution in many different lineages. Sex chromosomes often cease to recombine and therefore degenerate, and have also often been reshuffled during their evolution from autosomes. It has been discovered that within Sylvioidea passerine species half of chromosome 4a has translocated and fused with

"man kan ha en liten ursäkt för att slåss om man är riktigt arg" : En intervjustudie om våldets orsaker och åtgärder

Studien utgår från förekomsten av våld i samhället och våld som ett pedagogiskt problem. Syftet är att undersöka ung-domars och lärares attityder till fenomenet våld för att kunna anpassa preventiva åtgärder därefter. I studien an-vänds en kvalitativ ansats för att kartlägga hur elever och lärare på en skola uppfattar och beskriver våld, dess orsaker och möjligheterna att förebygga det. Datainsaml

Constructions of Multiculturalism, Voice and Identity in News Media Representations during Australia Day 2016

The national day of Australia is Australia Day; a contentious public holiday that sparks a national debate on identity and multiculturalism every year. There are many different narratives that make up the Australian story, which encapsulates Australia’s diverse and multicultural history. Media plays a pivotal role in the representation of the national narrative; however, evidence of systemic power