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Comparing two microarray platforms for identifying mutated genes in barley (Hordeum vulgare L.)

We have previously described the evaluation of a cDNA microarray platform to identify and clone mutated barley (Hordeum vulgare L.) genes, using their transcriptionally defective mutant alleles (S. Zakhrabekova, C.G. Karmangara, D. von Wettstein, M. Hansson, A microarray approach for identification of mutated genes, Plant Physiol. Biochem. 40 (2002) 189-197). It was concluded that competitive hybr

Erfarenheter av föräldraskap hos kvinnor och män med Cystisk Fibros

Today most patients with CF reach adulthood and consequently marry and have children. Health care systems are lacking in knowledge of how these adults cope with the strain of having children. Eight patients were interviewed about their experiences of having children. The study was a qualitative descriptive study and the patients were interviewed with open questions regarding their experiences of p

Severe defect in thymic development in an insertional mutant mouse model

Transgenic mice were generated expressing NK1.1, an NK cell-associated receptor, under control of the human CD2 promoter. Unexpectedly, one of the founder lines, Tg66, showed a marked defect in thymic development characterized by disorganized architecture and small size. Mapping of the transgene insertion by fluorescence in situ hybridization revealed integration in chromosome 2, band G. Already f

Effects of ions on partitioning of serum albumin and lysozyme in aqueous two-phase systems containing ethylene oxide/propylene oxide co-polymers

Aqueous two-phase systems composed of ethylene oxide/propylene oxide random co-polymers, EO30/PO70 or Ucon (EO50/PO50), in the top phase and dextran T500 in the bottom phase, have been studied. The cloud point diagram for EO30/PO70 in water solution was determined. EO30/PO70 has a cloud point of 32oC at a concentration of 10% (w/w). The phase diagram for the system EO30/PO70-dextran T500-water was

Hypoxia and differentiation in human neuroblastoma cells

The childhood tumour neuroblastoma is derived from immature cells of the sympathetic nervous system, which have become arrested at different maturation stages. Neuroblastoma is a malignancy with a high degree of heterogeneity, and there is a correlation between a poor differentiation status and a more aggressive phenotype. The MYCN gene is amplified in approximately 25% of neuroblastoma tumours an

Pubertal bone growth in the femoral neck is predominantly characterized by increased bone size and not by increased bone density-a 4-year longitudinal study.

Fragility fractures are correlated to reduced bone size and/or reduced volumetric bone density (vBMD). These region-specific deficits may originate from reduced mineral accrual and/or reduced skeletal growth during the first 2 decades of life. Before pathological development can be defined, normal skeletal growth must be described. To evaluate growth of bone size, accrual of bone mineral content (

Anställningen vid verksamhetsövergång (The Employment in the Event of Transfer of Undertaking)

Popular Abstract in Swedish Sedan Sverige för ungefär tio år sedan blev medlem av Europeiska unionen, änd-rades svensk arbetsrätt så att arbetstagare i vissa fall har en rätt att välja att följa med en verksamhet som övergår till en ny arbetsgivare. Något nytt anställningsavtal skall inte anses ha träffats. De arbetstagare som berörs av verksamhetsövergången har dessutom ett skydd mot att bli uppsWhen Sweden in 1995 became a member of the European Union, Swedish labour legislation was enacted on the safeguarding of employees’ rights in the event of transfer of undertaking. These Swedish rules and regulations are examined. EU law and national law form two parallel systems, which between themselves arrange the legal sources hierarchically, and, as a result of that, the Member States have tra

Grammatiken och språknormerna

The article treats the problem why a descriptive grammar cannot avoid from being normative in some ways. some uses of language are always excluded. on the other hand a descriptive grammar should account for competing variants where there might be disagreement among the language users which expression is correct, i.e. should be accepted as norm. The descriptive grammar does not tell which variant i

Long-term exposure to ethanol increases the number and function of muscarinic M1 receptors in human neuroblastoma cells

The effect of long-term ethanol exposure on muscarinic receptors was investigated in human neuroblastoma SH-SY5Y cells. Exposure to 100 mM ethanol for 4 days enhanced both peak and steady-state levels of carbachol-stimulated inositol 1,4,5-bisphosphate increase. An ethanol concentration of 50 mM was sufficient for an enhancement of this event. The carbachol-stimulated decrease in [3H]inositol-labe