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This thesis aims to produce and characterize endo-xylanases from the glycoside hydrolase family 5 (GH5) to hydrolyze corn arabinoxylan and produce short-chain oligosaccharides. Five new enzymes from family GH5 were tested, CtXyl5A from Clostridium thermocellum and the GpXyl5 from the fungal GH5_34 gave the best results. DNS assay was performed for the determination of the optimal conditions of the
