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Platelet-derived growth factor promotes survival of rat and human mesencephalic dopaminergic neurons in culture

The effect of two isoforms of platelet-derived growth factor (PDGF), PDGF-AA and PDGF-BB, was tested on dissociated cell cultures of ventral mesencephalon from rat and human embryos. PDGF-BB but not PDGF-AA reduced the progressive loss of tyrosine hydroxylase- (TH)-positive neurons in rat and human cell cultures. The mean number of TH-positive cells in the PDGF-BB-treated rat culture was 64% and 1

Annual CO2 exchange between a nutrient-poor, minerotrophic, boreal mire and the atmosphere

[1] Mires are key landscape elements at high latitudes and have certainly accumulated carbon during the Holocene, but their current carbon balance at the present time is very unclear. The major carbon flux is the land-atmosphere CO2 exchange and full-year data are still limited. Here we present data from 3 a (2001-2003) of continuous Eddy Covariance measurements at Degero Stormyr (64 degrees 11'N,

Genetic Variation in Sex Hormone Genes Influences Heel Ultrasound Parameters in Middle-Aged and Elderly Men: Results From the European Male Aging Study (EMAS)

Genes involved in sex hormone pathways are candidates for influencing bone strength. Polymorphisms in these genes were tested for association with heel quantitative ultrasound (QUS) parameters in middle-aged and elderly European men. Men 40-79 yr of age were recruited from population registers in eight European centers for the European Male Aging Study (EMAS). Polymorphisms were genotyped in AR, E

Voluntary wheel running modulates glutamate receptor subunit gene expression and stress hormone release in Lewis rats

Lewis rats that are known to be addiction-prone, develop compulsive running if they have access to running wheels. The present experiments were aimed 1) to evaluate the activation of stress systems following chronic and acute voluntary wheel running in Lewis rats by measurement of hormone release and gene expression of neuropeptides related to hypothalamic-pituitary-adrenocortical (HPA) axis activ

Effect of Heat Treatment on the Circular Dichroism Spectra of Bovine β Lactoglobulin A, B and C.

Dilute solutions of -lactoglobulin (-Lg) A, B, and C were heated in phosphate buffer at temperatures between 40 and 94 C for 10 min, cooled, and analyzed using near-UV and far-UV circular dichroism (CD). The decrease in near-UV CD intensity at 293 nm (293) could be analyzed in terms of a two-state model, and the stability was -Lg C > -Lg A > -Lg B on the basis of the midpoint temperatures for samp

The N-terminal N5 subdomain of the alpha 3(VI) chain is important for collagen VI microfibril formation

Collagen VI assembly is unique within the collagen superfamily in that the alpha 1(VI), alpha 2(VI), and alpha 3(VI) chains associate intracellularly to form triple helical monomers, and then dimers and tetramers, which are secreted from the cell. Secreted tetramers associate end-to-end to form the distinctive extracellular microfibrils that are found in virtually all connective tissues. Although

Reduced grazing rates in Daphnia pulex caused by contaminants: Implications for trophic cascades

Ecotoxicological endpoints based on behavioral traits (e.g., predator avoidance, feeding, and locomotion) may be more sensitive and give more insights into patterns of sublethal toxicity than survivorship tests. In this study, the density-dependent grazing rate of Daphnia pulex pre-exposed to p,p'-dichlorodiphenyldichloroethylene (DDE) (insecticide metabolite) and glyphosate (herbicide), via water

Applications of two-photon absorption for detection of CO in combustion gases

Laser-induced fluorescence has been used for detection of CO in different environments. The fluorescence light was obtained by using a two-photon transition between theX1?- and theB1?- electronical states around 230 nm. Cell measurements indicate a detection limit lower than 0.1 ppm. Measurements in a CH4/air flame and in a low pressure dc discharge were realized with a diode-array detector, which

Alteration of intestinal microflora is associated with reduction in abdominal bloating and pain in patients with irritable bowel syndrome

OBJECTIVE: The influence of the gastrointestinal (GI) microflora in patients with irritable bowel syndrome (IBS) has not been clearly elucidated. This study was undertaken to see if patients with IBS have an imbalance in their normal colonic flora, as some bacterial taxa are more prone to gas production than others. We also wanted to study whether the flora could be altered by exogenous supplement

Predicting the Displacement of Yogurt by Water in a Pipe Using CFD

A numerical scheme based on the volume of fluid (VOF) method for predicting the displacement of one liquid by another has been verified versus electrical resistance tomography (ERT) and ultrasonic velocity profile (UVP) measurements for the displacement of yoghurt by water. The scheme using the VOF method predicts the skewed phase distribution as measured using ERT and the global structure of the

International Biofuel Trade - A Study of the Swedish Import

Following the development of large-scale use of biomass energy in the EU, international biofuel trade is a plausible scenario and something that is already taking place in Northern Europe. This paper focuses on Swedish biofuel imports, both direct and indirect imports, the latter which derive from the fact that part of the imported pulpwood and timber end up as fuel. The objective is to describe t

Transport properties and aggregation phenomena of polyoxyethylene sorbitane monooleate (polysorbate 80) in pig gastrointestinal mucin and mucus

The aqueous environment in the gastrointestinal tract frequently requires solubilization of hydrophobic drug molecules in appropriate drug delivery vehicles. An effective uptake/absorption and systemic exposure of a drug molecule entails many processes, one being transport properties of the vehicles through the mucus layer. The mucus layer is a complex mixture of biological molecules. Among them,

Precise in situ thickness analysis of epitaxial graphene layers on SiC(0001) using low-energy electron diffraction and angle resolved ultraviolet photoelectron spectroscopy

We demonstrate an easy and practical method for the thickness analysis of epitaxial graphene on SiC(0001) that can be applied continuously during the preparation procedure. Fingerprints in the spot intensity spectra in low energy electron diffraction (LEED) allow for the exact determination of the number of layers for the first three graphene layers. The LEED data have been correlated with the ele

Identification, synthesis and activity of sex pheromone gland components of the autumn gum moth (Lepidoptera : Geometridae), a defoliator of Eucalyptus

The autumn gum moth, Mnesampela privata (Guenee) (Lepidoptera: Geometridae), is native to Australia and can be a pest of plantation eucalypts. Field-collected and laboratory-reared female autumn gum moths were dissected to remove glands likely to contain components of the sex pheromone. Using gas chromatography (GC) and combined gas chromatography-mass spectrometry (GC-MS), three compounds were id

Experimental observation of different-order components of a vibrational wave packet in a bulk dielectric using high-order Raman scattering

We use high-order Raman scattering in a bulk dielectric to characterize coherent dynamics with precision typical for gas phase experiments. The experimental pump-probe approach allows for the simultaneous observation and separation in space and time of the individual contributions of different-order Raman processes to a coherent wave packet without relying on phase-matching conditions and within t