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Popular Abstract in Swedish Blodbildande stamceller förekommer i mänsklig benmärg i en frekvens på ca 1/100 000 celler. Med hjälp av cellytemarkörer, t.ex. CD34, kan man rena fram cellpopulationer som är anrikade på stamceller, men det saknas exakt kunskap om hur enskilda stamceller ser ut. De blodbildande stamcellerna har förmåga att vid celldelning antingen förnya sig själva eller att mogna ut. Hematopoietic stem cells (HSCs) have the capacity to maintain hematopoiesis throughout life. HSCs are ideal targets for permanent gene transfer, as the transgene will be expressed in all the progeny of the gene-modified stem cells. The aim of this thesis was to optimize conditions for retroviral gene transfer to human candidate HSCs and to study mechanisms interfering with efficient gene transfer.
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Abstract in German Der Esssay behandelt Fakten und Phantasien und lässt dabei die Linien zwischen beiden Genres ineinander laufen - so wie historische Romane, Dramen und Opern tatsächlich arbeiten. Die Motive sind unterschiedlich, dienen aber meist einer interpretativen Verdeutlichung zum besseren Verständnis einer aktuellen Problemkonstellation. Mitunter werden aber auch Dramen oder Opern - nicht
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Despite the recognised importance of the concept of acceptance, how and why new technologies are actually accepted by drivers is not well understood. While many studies claim to have measured acceptance, few have defined what it is. The chapter points out the importance of defining acceptance and categorizes the definitions that have been used according to their “essence” and the various dimension