Interactions Between Small Heat Shock Proteins and Substrate Proteins Mass Spectrometric Detection of Crosslinked Peptides to Map Protein-Protein Interactions
The small heat shock proteins (sHsps) are a widespread class of molecular chaperones existing in all organisms. During heat stress the sHsps protect other proteins from unfolding and aggregation. In plants the sHsps play an especially prominent part of the stress response. In this thesis chemical crosslinking and mass spectrometric mapping of crosslinked peptides has been used to investigate the
