Engineering protein-carbohydrate interactions - lessons from natural and evolved carbohydrate binding modules
Protein-carbohydrate interactions are of central role in all living organism. In the studies presented in this thesis several strategies for engineering such interactions have been investigated. In the first study the binding affinity of a carbohydrate binding module (CBM) was improved a 100-fold through affinity maturation. Also, in that study it was discovered that all the selected, matured muta