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Engineering protein-carbohydrate interactions - lessons from natural and evolved carbohydrate binding modules

Protein-carbohydrate interactions are of central role in all living organism. In the studies presented in this thesis several strategies for engineering such interactions have been investigated. In the first study the binding affinity of a carbohydrate binding module (CBM) was improved a 100-fold through affinity maturation. Also, in that study it was discovered that all the selected, matured muta

Optimal Linear Joint Source-Channel Coding with Delay Constraint

The problem of joint source-channel coding is considered for a stationary remote (noisy) Gaussian source and a Gaussian channel. The encoder and decoder are assumed to be causal and their combined operations are subject to a delay constraint. It is shown that, under the mean-square error distortion metric, an optimal encoder-decoder pair from the linear and time-invariant (LTI) class can be found

An extremum seeking approach to parameterised loop-shaping control design

An approach to loop-shaping feedback control design in the frequency domain via extremum seeking is proposed. Both plants and controllers are linear time-invariant systems of possibly infinite dimension. The controller is assumed to be dependent on a finite number of parameters. Discrete-time global extremum seeking algorithms are employed to minimise the difference between the desired loop shape

B2B rather than B2C to Meet Customer Demands in the Pharmaceutical Retail Industry

An increased use of the Internet has created new business opportunities. The development of e-commerce has, however, been crippled by a limited understanding of logistics and customer needs. This paper sets out to highlight those issues in pharmaceutical retail where added services should be created through e-commerce. The research is based upon a case study covering the pharmaceutical system in S