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Kloning, överuttryck och upprening av växtakvaporinet AtSIP1;1 i Escherichia coli

En ny underfamilj av cellulära vakter i växter Heterologt överuttryck av proteiner innebär att en gen som kodar för ett protein uttrycks i en annan värdorganism som normalt inte har den genen i sin arvsmassa. Inom biokemisk forskning är detta en viktig metod för att kunna studera proteiners struktur och funktion. Tyvärr kan uttrycket av en främmande gen medföra skador på värdorganismens celluläraHeterologous overexpression of proteins signifies that the encoding gene of a protein in one organism is expressed in another host organism that normally does not have that gene in its genome. In basic biochemical research, this is an important method for studying various protein functions such as activity and interactions in biological processes, as well as obtaining sufficient amounts to determi

Businesses’ Willingness to Sustainable Approach Using BMfS to Manage Sustainable Supply Chain Evidence of Development and Implementation of BMfS on Introduction of Organic Cotton Action Outdoor Products

With the rapid production of sportswear and its price advantage, consumption has also increased significantly, which has a rather negative impact on the environment, society, and the economy. Fashion retailers and their consumers are also well aware of this, and their demands for sustainable fashion have had a strong impact on the production of sportswear and consumer behaviour in recent years. Su

Ascribed Meanings to Self- Service Technologies: Exploring Grocery Shopper’s Use of SSTs and Subsequent Meanings held in a Digitalised World

Recent years have seen digitalisation entangled in everyday life; with the broader context of a digitalised world, one can no longer see the digitalised retail space and its technological artefacts as a lone-standing phenomena. Instead, it is necessary to see digitalisation as way of understanding and being in the modern world. This paper explores the practices and subsequent meanings in the use o

Kollektivtrafikens bidrag till stadens hållbara tillgänglighet - En analys av restidskvoter i Malmö

I ett hållbart samhälle är tillgänglighet en viktig förutsättning för alla, oavsett deras bakgrund. Det finns olika komponenter som ingår inom begreppet tillgänglighet. En av dessa komponenter handlar om transportmöjligheten, att man ska ha möjlighet att transportera sig till olika punkter med minsta möjliga reseuppoffring. Detta kan uppnås med hjälp av kollektivtrafiken, eftersom kollektivtrafik In a sustainable society accessibility is vital and should be provided for everyone, regardless of their background. There are different components within accessibility as a concept. One of these components is transport, which has to do with the ability to transport to different destinations with minimum sacrifice. This can be achieved with public transport, because it’s available for everyone and

Construction of monotone smoothing splines using a branch and bound method

The purpose of this report is to investigate a branch and bound algorithm designed to construct monotone smoothing splines. The splines are defined as the solution of a minimization problem, where one wants to find a curve with non-negative derivative everywhere, that fits a data set consisting of points in the plane, while also being sufficiently smooth. The report describes the theory necessary

Segmentation of the Common Carotid Artery from Ultrasound Images using UNet

The common carotid artery (CCA), the artery that supplies our brains with oxygen, is of great importance in stroke research. The usual way to monitor the artery is by using ultrasound (US) imaging. An automated way of segmenting out the arteries from the US images is desired to optimize research. With an upswing in development of convolutional neural networks (CNN), this is now possible. CNN uses

Distansstudier vid Lunds universitet- en möjlighet till social rörlighet? En kvantitativ undersökande studie om distansstudier ur ett klassperspektiv.

Tidigare forskning visar att distansstudier vid högskolor och universitet har blivit allt vanligare. Den tidigare forskningen visar även att denna form av studier är vanligare hos individer från ett arbetarklasshem samt hos individer med föräldrar/vårdnadshavare som saknar högre studier. Syftet med denna studie var att utöka kunskapen kring distansstudier som en möjlighet till social rörlighet.

What is required by software platforms in order to give a good developer experience?

Vi lever idag i ett digitalt samhälle. Du använder förmodligen själv dagligen olika program och även om all mjukvara i slutändan består av ettor och nollor finns det allt som oftast en person bakom varje program som tagit fram den, en utvecklare. Programmerare tar ofta hjälp från så kallade mjukvaruplattformar. Dessa riktar sig inte direkt mot vanliga användare, utan mot just utvecklare av mjukvaThis research report was carried out in collaboration with Qlik AB and aimed to identify what aspects are needed from a software platform in order for developers to want to use them. It also aimed to identify how well the software platform Qlik Core had implemented these aspects. The research used online questionnaires, interviews and data analysis using Qlik Sense. The research compared how diffe

Derivation of a transfer function to estimate the Z(vv)+jets background from photon+jets in a search for dark matter at the ATLAS detector

It has for long been known that dark matter must exist in our universe. Despite massive efforts to detect dark matter, its constituents remain a mystery. One strategy to unveil this mystery is to produce dark matter by colliding Standard Model particles. The most common signature of dark matter production is an excess of missing transverse energy, MET. Such an event would be very rare, which is wh

Fenomenet elsparkcykel- en studie där användarna belyser värdeskapandet av elsparkcykeln

Elsparkcykel har blivit ett populärt fenomen i Sverige där flera företag erbjuder uthyrning av detta transportmedel. Vid studiens tidpunkt kunde konstateras att få hade studerat fenomenet och vad användarna ansåg vara av värde med elsparkcykeln, det fanns därmed ett kunskapsgap inom området. Syftet med denna studie blev därför att öka förståelsen för vad användarna ansåg vara av värde med användan

Variationen av kväve- och fosforhalten i olika biflöden till Hagaån

Höga näringshalter har uppmätts i Hagaån på Orust, Västra Götaland och kan i framtiden leda till övergödning och därmed ge förödande effekter på ekosystemet. Fisk har redan börjat minska på västkusten och en anledning kan vara näringsläckage från jordbruksmark. Det är allmänt känt, att övergödning är ett stort miljöproblem i våra sjöar och hav. Mänskliga aktiviteter som exempelvis jordbruk och avEutrophication of aquatic environments is a worldwide problem which is usually caused by leakage of nutrients from human activities on land. The primary production rises greatly when concentrations of phosphor and nitrogen increases, which can result in disturbances in the ecosystems. The aim of this project was to analyse the content of total-phosphor and total-nitrogen in different flow rates in

Självuttorkning i betong med aluminatrika tillsatsmaterial

Tonvis av betong används på svenska byggen varje år. Detta trots att tillverkningen av en av huvudingrediensenserna - cement - ger stora mängder koldioxidutsläpp. Genom att ersätta en del av cementet med ett visst specialcement minskar koldioxidutsläppen samtidigt som betongen torkar fortare. För de flesta höghus som byggs i Sverige idag används betong som byggmaterial. Enorma mängder betong gårThis master thesis work concerns the effect of high-alumina additives on the selfdesication of concrete. The purpose of the work was to examine the possibilities to optimize the binder-content of concrete with regards to self-desiccation. Two highalumina additives have been examined. In the experiments 10% of the binder mass has been replaced with one of these additives. Commercially available ceme

The Systematic Risk of Green Bonds

This paper evaluates the profitability of adding green bonds to a portfolio consisting of stocks and conventional bonds. To determine the profitability, ten portfolios with varying weights on green bonds, conventional bonds and stocks, are constructed. The risk-adjusted returns on the portfolios are implemented in time series regressions of several asset pricing models, including the Capital Asset

A first look at some potentially plastic degrading enzymes

Med ordet ’plast’ avses i vardagligt bruk typiskt helt eller delvis syntetiskt framställda polymerer (långa kedjor av upprepande mindre enheter), ofta tillverkade av ursprungligen fossilt råmaterial. Tack vare det stora omfång av önskvärda egenskaper som plaster generellt besitter, däribland uthållighet mot biologisk och kemisk nedbrytning samt låga produktionskostnader, har plaster blivit allstädThe many advantageous abilities of plastics have made them omnipresent in today’s society. However, the low cost of production, light weight, durability and insolubility in water combined with inadequate waste handling have led to a vast and increasing accumulation of plastics in nature, approximated to be over 6 300 million tons (Geyer, Jambeck and Law, 2017, ‘Production, use, and fate of all pla

Daylight and energy performance of a double and single skin facade in a cold climate

The objective of this thesis was to investigate how double skin facades (DSFs) meet daylight and energy requirements compared to single skin facades (SSFs), also considering the life cycle costing (LLC) of both solutions. A South facing, an unobstructed glazed office building in Sweden was used as a basic building object, where SSFs and DSFs were iteratively applied. A mid-level floor was chosen a

The vocal citizens of the European Parliament Facebook page: Who are they and why do they get engaged online?

Digital media and social media are by now established platforms for daily online social interaction, expression and debate, also in the political field. They offer users a space to voice and exchange opinions, as well as a tool to organise political acts of protest or support around offline world events. And they are changing the game of politics in remarkable ways. Political institutions such as

Putting people back into the economy: Urban restructuring and emerging alternatives in time of crisis

In many cities around the world, the financial crisis 2007 served as an opportunity both for grassroots movements and governments to rethink existing models of urban development. Some authors have pointed out that the crisis served to elevate discourses for progressive post-neoliberal urban transformations. Other, more sceptical accounts have pointed at the shift towards participative, networked g

Adaptive content-based sound compression

Smart komprimeringsteknik för ljudinspelningar Omfattande forskning inom videokomprimering har lett till en märklig balans där ljudspåret ibland tar större plats än videospåret i övervakningsinspelningar. För att jämna ut detta behövs smarta tekniker även för ljudkomprimering. Det här arbetet presenterar ett förslag för att minska ljuddatan med mer än 50 %. Det är vanligt att majoriteten av mateThree different classification solutions for distinguishing events from non-events in surveillance audio are described and evaluated. The three compared methods are energy functions, Gaussian mixture modelling algorithms (GMM) and existing voice activity detectors (VAD). Recorded test signals with corresponding manually labelled ground truth files are used to determine the accuracies of the method

Clustering and Anomaly Detection in Financial Trading Data

In this thesis we propose a new form of Variational Autoencoder called the Conditional Latent Space Variational Autoencoder or CL-VAE. By conditioning on a known label in a dataset we can decide what points are being mapped to what prior distribution. This makes the latent space more understandable and separates the classes further. It also subverts the tug-of-war effect between reconstruction los

Är polylaktid (PLA) framtidens plast?

Polylaktid (PLA)- en plast som har dålig nedbrytningsförmåga och borde kallas något annat Plasten polylaktid, PLA, marknadsförs som bionedbrytbart, någonting som faktiskt inte verkar stämma enligt olika studier. Plasten bryts inte alls ner under samtliga förhållanden i naturen utan kräver speciella anläggningar för fullständig nedbrytning. Inte heller tycks PLA uppfylla den europeiska standarden fThis paper aims to look at the positive and negative aspects of the biodegradable material poly(lactic) acid (PLA) compared to controversial plastics. This is done through a literature study. PLA is a biobased and biodegradable plastic that are used in for example medicine and packaging materials around the world. The results from this study show that the biodegradation of the material is not that