Genomic manipulation of Yersinia ruckeri, a pathogen of salmonid fish species, is essential forunderstanding bacterial physiology and virulence. Here, we present a protocol for genomicrecombineering in Y. ruckeri, a species reluctant to standard genomic engineering, using CRISPRCas12a coupled with the l Red system. We describe steps for identifying protospacer guides,preparing repair template plasGenomic manipulation of Yersinia ruckeri, a pathogen of salmonid fish species, is essential for understanding bacterial physiology and virulence. Here, we present a protocol for genomic recombineering in Y. ruckeri, a species reluctant to standard genomic engineering, using CRISPR Cas12a coupled with the λ Red system. We describe steps for identifying protospacer guides, preparing repair template

We've long focused on the green transition, discussing various systems emphasizing the technical aspects of transitioning our lives. Yet, as many people experience anxiety about climate change, other, more existential perspectives are rising to the surface. Questions such as What is a good life? becomes more pressing as we begin to feel the effects of climate change also in our part of the world.

The invention relates to re-engineering of a natural microbial syringe for loading of effector proteins that are not naturally found as cargo of the specific syringe. The cargo, that is one or more effector protein and proteins required for packing, can be up to 300 kDa and can be proteins such as toxins, therapeutic proteins, and gene modification tools. The re-engineering of the natural syringe

The thyroglobulin (TG) protein is essential to thyroid hormone synthesis, plays a vital role in the regulation of metabolism, development and growth and serves as intraglandular iodine storage. Its architecture is conserved among vertebrates. Synthesis of triiodothyronine (T3) and thyroxine (T4) hormones depends on the conformation, iodination and post-translational modification of TG. Although stThe thyroglobulin (TG) protein is essential to thyroid hormone synthesis, plays a vital role in the regulation of metabolism, development and growth and serves as intraglandular iodine storage. Its architecture is conserved among vertebrates. Synthesis of triiodothyronine (T3) and thyroxine (T4) hormones depends on the conformation, iodination and post-translational modification of TG. Although st

Valid measurement instruments are needed to investigate the impact of parental bonding on child health development. The aim was to develop and validate a self-report questionnaire, the Parent-to-Infant Bonding Scale (PIBS) to measure bonding in both mothers and fathers. Internal consistency and construct validity were analysed using data from Swedish parents from both clinical (N = 182), and commu

In 2020, the European Parliament (ep) issued a resolution calling for all European Union (EU) Member States to adopt feminist foreign and security policies, and for a gender-transformative vision in the EU’s own external policy. Drawing on the literatures on negotiations and norm contestation, this article3 asks why the resolution was so progressive. It also asks how we can characterize and explai

Introduction Deep learning models for detecting episodes of atrial fibrillation (AF) using rhythm information in long-term ambulatory ECG recordings have shown high performance. However, the rhythm-based approach does not take advantage of the morphological information conveyed by the different ECG waveforms, particularly the f-waves. As a result, the performance of such models may be inherently l

Tree establishment on peatlands has various adverse effects on the environment, with one of the most significant being their transformation from carbon sinks to carbon sources. This transformation has largely been instigated by economic-driven ditching initiatives. In this study, 80 peat-rooted pine seedlings were subjected to hydrological scenarios corresponding to natural, ditched, and rewetted

Women constitute most volunteer water stewards in West Virginia. After having conducted participatory research on the motivations behind women’s engagement with water preservation and restoration work we carried out two participatory art-based activities. In this Practices and Curations, we reflect on these two art-based activities to facilitate networking between researchers and participants and

Background and aims: Despite firm evidence for an association between long-term ambient air pollution exposure and cardiovascular morbidity and mortality, results from epidemiological studies on the association between air pollution exposure and atherosclerosis have not been consistent. We investigated associations between long-term low-level air pollution exposure and coronary atherosclerosis. Me

Dr. Dalia Dassa Kaye is a 2023-2024 Fulbright Schuman scholar based at the Centre for Advanced Middle Eastern Studies at Lund University, Sweden and at the German Marshall Fund in Brussels, Belgium as a non-resident senior fellow. Dalia’s research compares American and European approaches to Iran beyond the nuclear agreement. She is a senior fellow at the UCLA Burkle Center for International Relat

Objective: Subsets of CD21−/low memory B cells (MBCs), including double-negative (DN, CD27−IgD−) and Tbet+CD11c+ cells, are expanded in chronic inflammatory diseases. In rheumatoid arthritis (RA), CD21−/low MBCs correlate with joint destruction. However, whether this is due to the Tbet+CD11c+ subset, its function and pathogenic contribution to RA are unknown. This study aims to investigate the ass

Objective: Hand osteoarthritis (OA) pain is characterized as heterogeneous and multifactorial. Differences in pain may be explained by underlying phenotypes, which have not been previously explored Design: Latent class analysis determined classes of participants with hand OA from the Nor-Hand study baseline examination (2016–17) based on a biopsychosocial framework. Outcomes were hand and overall

Introduction: There is a need for new therapies that can enhance response rates and broaden the number of cancer indications where immunotherapies provide clinical benefit. CD40 targeting therapies provide an opportunity to meet this need by promoting priming of tumor-specific T cells and reverting the suppressive tumor microenvironment. This is supported by emerging clinical evidence demonstratin

In post-quantum cryptography (PQC), Learning With Errors (LWE) is one of the dominant underlying mathematical problems. For example, in NIST's PQC standardization process, the Key Encapsulation Mechanism (KEM) protocol chosen for standardization was Kyber, an LWE-based scheme. The primal and the dual attacks are the two main strategies considered for solving the underlying LWE problem of multiple

a documentary performance about the life and working conditions in the berry picking industry by Fix+Foxy (DK)

Students’ pre-knowledge and conceptual development in analysis were investigated at a teacher education program to reveal what pre-knowledge endured and how the students perceived the concepts a year after the course had ended. Questionnaires and interviews were used to collect data. Two students’ results are presented in more detail in the article. The study was cognitively framed with the influe