A Green Alternative for the Development of Blood Substitutes
BvHb1.2 is a sugar beet haemoglobin (Hb) and a good candidate to be used as an artificial oxygen carrier (AOC). 19 mutants of BvHb1.2 were produced with random mutagenesis and expressed in BL-21 E. coli. The Hb content in whole cells was determined by using a refraction index modifier by mixing cell suspensions with 35% BSA. The two mutants (4 and 15) with the highest Hb production were purified u