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Epigenetic silencing defends against LINE-1 (L1) retrotransposition in mammalian cells. However, the mechanisms that repress young L1 families and how L1 escapes to cause somatic genome mosaicism in the brain remain unclear. Here we report that a conserved Yin Yang 1 (YY1) transcription factor binding site mediates L1 promoter DNA methylation in pluripotent and differentiated cells. By analyzing 2

The retrotransposon Long Interspersed Element 1 (LINE-1 or L1) is a continuing source of germline and somatic mutagenesis in mammals. Deregulated L1 activity is a hallmark of cancer, and L1 mutagenesis has been described in numerous human malignancies. We previously employed retrotransposon capture sequencing (RC-seq) to analyze hepatocellular carcinoma (HCC) samples from patients infected with he

LINE-1 (L1) retrotransposons are a noted source of genetic diversity and disease in mammals. To expand its genomic footprint, L1 must mobilize in cells that will contribute their genetic material to subsequent generations. Heritable L1 insertions may therefore arise in germ cells and in pluripotent embryonic cells, prior to germline specification, yet the frequency and predominant developmental ti

The mouse genome is replete with retrotransposon sequences, from evolutionarily young elements with mutagenic potential that must be controlled, to inactive molecular fossils whose sequences can be domesticated over evolutionary time to benefit the host genome. In an exciting new study, de la Rica and colleagues have uncovered a complex relationship between ten-eleven translocation (TET) proteins

BACKGROUND: LINE-1 (L1) retrotransposons are a notable endogenous source of mutagenesis in mammals. Notably, cancer cells can support unusual L1 retrotransposition and L1-associated sequence rearrangement mechanisms following DNA damage. Recent reports suggest that L1 is mobile in epithelial tumours and neural cells but, paradoxically, not in brain cancers.RESULTS: Here, using retrotransposon capt

Transposable elements (TEs) are notable drivers of genetic innovation. Over evolutionary time, TE insertions can supply new promoter, enhancer, and insulator elements to protein-coding genes and establish novel, species-specific gene regulatory networks. Conversely, ongoing TE-driven insertional mutagenesis, nonhomologous recombination, and other potentially deleterious processes can cause sporadi

Human induced pluripotent stem cells (hiPSCs) are capable of unlimited proliferation and can differentiate in vitro to generate derivatives of the three primary germ layers. Genetic and epigenetic abnormalities have been reported by Wissing and colleagues to occur during hiPSC derivation, including mobilization of engineered LINE-1 (L1) retrotransposons. However, incidence and functional impact of

This article analyzes Paul Celan’s translation of James Baldwin’s “Everybody’s Protest Novel” and considers the connections between the poetics of these two writers. In addition to their shared preoccupation with and rearticulation of terms such as human, creature, and darkness, the translation reveals how they explored the relationship between literature and reality at early and transitional mome

Superporous agarose beads were used as an affinity support in column chromatography. These beads characteristically possess two sets of pores, normal diffusion pores and flow pores, so-called superpores. The superpores, whose diameter is a substantial fraction of the particle diameter (i.e. 1/3 to 1/10 of the particle diameter), allow part of the chromatographic flow to pass through each individua

This article centres on Bertha Pappenheim’s translations of Old Yiddish literature, with particular focus on Die Memoiren der Gl€uckel von Hameln, which was published as a private edition in Vienna in 1910. While the fact that Pappenheim translated Glikl’s Yiddish-language writings into German is well known, little attention has been paid to the particular language of the translated text or to Pap

This article reevaluates the circulation and reception of the memoirs of Glikl, an early modern Jewish writer and businesswoman, in the German-speaking world from the mid-19th to the early 20th century. It considers how the text’s modern editors and translators – including Abraham Tendlau, David Kaufmann, Bertha Pappenheim, and Alfred Feilchenfeld – shaped its meaning and determined its value over

Superporous particles contain two sets of pores, normal diffusion pores and very large flow pores. If such particles are used chromatographically, part of the flow will pass through each particle. This leads to a rapid equilibration between the stationary and the mobile phases, even if the superporous particle has a large outer diameter. Superporous particles are thus chromatographically efficient

This dissertation shows how the intersection between German and Yiddish became an important but largely forgotten site of literary production in the long twentieth century. Beginning in the late eighteenth century, German and Yiddish came to be associated with divergent trajectories of Jewish modernity and were often understood to represent either side of a series of charged dichotomies, such as W

This paper reports on a new type of spherical agarose chromatography particles characterized by two sets of pores, normal diffusion pores, characteristic of all agarose materials and very wide pores, so-called superpores or flow pores. These superpores allow part of the chromatographic flow to pass through each individual particle, which gives improved mass transfer, especially in situations where

Numerous studies have reported a correlation between gut microbiota and influenza A virus (IAV) infection and disease severity. However, the causal relationship between these factors remains inadequately explored. This investigation aimed to assess the influence of gut microbiota on susceptibility to human infection with H7N9 avian IAV and the severity of influenza A (H1N1)pdm09 infection. A two-s

Ces dernières années en Ouganda, les écologistes ont fait l'objet de répressions partout dans le pays. Pour sauvegarder la nature, des journalistes, des étudiants, ainsi que des agriculteurs et des habitants de zones rurales, protestent et se mobilisent contre les injustices et les déplacements forcés causés aussi bien par les infrastructures utilisant des énergies fossiles que par l’agro-industri

Electron energy spectra have been measured that result from K-shell ionization of Kr by two different mechanisms: (1) photoionization and (2) internal conversion in the decay of the isomeric state of Kr83. It is demonstrated experimentally that these spectra, including satellites on the low-energy side of the primary 1s-electron peak, are identical. A theoretical interpretation of the identity of

This chapter summarizes my doctoral thesis about Universal Design (UD) in Sweden, contributing knowledge about the understanding, implementation, and co-creation of UD. The implementation of the UN Convention on the Rights of Persons with Disabilities in Sweden made UD a guiding principle, necessitating research about the practical side of UD. Using qualitative research methods, interviews and gro