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Chinese hamster ovary (CHO) cells in the plateau phase were seeded in the absence or presence of 5 mM 2-difluoromethylornithine (F(2)MeOrn), an enzyme-activated irreversible inhibitor of ornithine decarboxylase. The thymidine analogue bromodeoxyuridine (BrdUrd, 5 mu M) was added to the culture medium 30 min before sampling of the cells, which occurred 1-17 h after seeding. Using flow cytometry, co

Objective. To analyze the experience of a single centre using stent-grafts for treatment of complicated acute aortic type B-dissections (EVR-ABD). Design. Retrospective analysis of prospectively collected data from patients undergoing EVR-ABD between January 1997 and December 2004. Methods. EVR-ABD was performed in 31 patients (20 males, median age 74. years (IQR: 64-79)). Indications for treatmen

The biological phosphorus removal process is often implemented at plants by the construction of an anaerobic bio-p tank in front of the traditional N removing plant configuration. However, biological phosphorus removal is also observed in plant configurations constructed only for nitrogen removal and simultaneous or post-precipitation. The operational experience with this "accidental" biological p

A detailed investigation of the contribution from Coulomb effects to the observed mirror energy difference diagrams in nuclei in the lower part of the upper fp shell is presented by means of large-scale shell-model calculations.

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The Ku autoantigen has been implicated in a number of cellular functions including growth control, immunoglobulin gene rearrangement and DNA repair. A variant truncated form of Ku86, with an apparent molecular weight of 70 kDa, has been reported to be present in many human cell types. We have previously shown that the amount of variant Ku86 is strongly increased in human peripheral blood mononucle

We present ab initio calculations of the optical rotation of S-propylene oxide in both gas phase and solution using the coupled cluster methodology combined with a dielectric continuum description of the solvent. The coupled cluster calculations are performed using the CCS, CC2, CCSD and CC3 methods. None of the presented gas phase results are in accord with the experimental sign of the optical ro

The article presents the third study of a series that has been designed to manifest consciousness and to measure developed competence. The emphasis of the main hypothesis has been put on the student’s ability to adapt to the main idea of a given story and to express his comprehension verbally. In what way the two students of the previous two experiments have been able to accomplish the experimenta

Gene expression analyses were performed on 121 consecutive childhood leukemias (87 B-lineage acute lymphoblastic leukemias (ALLs), 11 T-cell ALLs and 23 acute myeloid leukemias (AMLs)), investigated during an 8-year period at a single center. The supervised learning algorithm k-nearest neighbor was utilized to build gene expression predictors that could classify the ALLs/AMLs according to clinical

Målsättningen för projektet är att studera uppvärmningsenergibehov, energiförbrukning och inomhusklimat i 31 radhuslägenheter i Malmö med gasvärme. Detta görs genom att studera energibalansen för lägenheterna, funktionen hos systemen med luftburen värme, funktionen hos gasvärmesystemen samt de boendes upplevelse av inneklimatet via enkäter.

Analytical models to calculate notch development and subsequent mass failure of dunes are presented. The notch evolution model is based on a transport equation for sediment from the dune and the sediment volume conservation equation, whereas the models of mass failure are derived using elementary engineering statics and soil mechanics. An empirical transport coefficient in the model describing the

We present an algorithm for the inverse scattering problem associated to a nonlinear equation arising in the study of the unidirectional propagation of waves in shallow water.

Serine proteases play a central role in defense against pathogens by regulating processes such as blood clotting, melanization of injured surfaces, and proteolytic activation of signaling pathways involved in innate immunity. Here, we present the functional characterization of the Drosophila serine protease Sp7 (CG3006) by inducible RNA interference. We show that Sp7 is constitutively expressed in

Autoignition with SI compression ratio can be achieved by retaining hot residuals, replacing some of the fresh charge. In this experimental work it is achieved by running with a negative valve overlap (NVO) trapping hot residuals. The experimental engine is equipped with a pneumatic valve train making it possible to change valve lift, phasing and duration, as well as running with valve deactivatio