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Mechanisms behind the faceting of catanionic vesicles by polycations: Chain crystallization and segregation

Vesicles composed of an anionic and a cationic surfactant, with a net negative charge, associate strongly with a hydrophobically modified polycation (LM200) and with an unmodified polycation with higher charge density (JR400), forming viscoelastic gel-like structures. Calorimetric results show that in these gels, LM200 induces a rise of the chain melting temperature (T-m) of the vesicles, whereas

Two-dimensional gel electrophoresis of proteins and peptides in bovine milk

Proteins and peptides in bovine milk and colostrum were separated by two-dimensional gel electrophoresis (2-DE). The proteins were separated in the first dimension by isoelectric focusing in the pH ranges 4-7 and 6-11, and in the second dimension by sodium dodecyl sulphate polyacrylamide gel electrophoresis on 12.5% T homogeneous gels. The peptides were separated in the pH range 3-10 in the first

Biological monitoring of methylhexahydrophthalic anhydride by determination of methylhexahydrophthalic acid in urine and plasma from exposed workers

OBJECTIVE: To investigate whether methylhexahydrophthalic acid (MHHP acid) in urine and plasma can be used as a biomarker for exposure to methylhexahydrophthalic anhydride (MHHPA). METHODS: MHHPA in air was sampled by Amberlite XAD-2 and analysed by gas chromatography (GC) with flame ionisation detection. MHHP acid in urine and plasma was analysed by GC with mass spectrometric detection. Workers o

Nt-proANP in plasma, a marker of salt sensitivity, is reduced in type 2 diabetes patients.

Objective. We recently showed that plasma concentration of N-terminal atrial natriuretic peptide (Nt-proANP) is strongly directly related to salt sensitivity. The aims of the present study were to test (i) whether plasma concentration of N-terminal brain natriuretic peptide (Nt-proBNP) is related to salt sensitivity and (ii) whether Nt-proANP, as a marker of salt sensitivity, differs between type

Hepatitis C superinfection in hepatitis C virus (HCV)-infected patients transplanted with an HCV-infected kidney

Hepatitis C virus (HCV) genotypes, determined by polymerase chain reaction with type-specific primers, were studied in 5 already HCV-infected patients receiving kidneys from HCV-infected cadaver donors. Three patients were investigated retrospectively using stored pre- and posttransplantation sera and followed 18-28 months after transplantation. Two recipients with HCV genotype 2b infection had re

Changes of a non-specific defence mechanism in blood serum of piglets induced by prenatal and postnatal administration of alpha-ketoglutarate

The aim of this study was to establish the influence of alpha-ketoglutarate (AKG), administered to pregnant sows from the 91(SI) d of pregnancy to farrowing, and then to piglets from birth to the 30(th) d of life, on lysozyme and ceruloplasmin activity, serum total protein content, and the WBC counts in blood of piglets, at the age of the 14(th) and 30(th) d of their postnatal life. The sows were

Varför är svensk popmusik så populär utomlands? Synpunkter på det ”svenska musikundret” och på det spontana i popmusikens musikaliska språk

This paper discusses the reasons for the recent success of Swedish popular music abroad. It departs from recent Swedish economic reports showing that the increase in exports of Swedish music per capita is comparable to, and even exceeds, those of the United Kingdom and the United States, and presents theories about why Swedish popular music exports have grown during the last years. A method of mu

Platination of the siRNA sense-strand modulates both efficacy and selectivity in vitro

The use of short interfering RNAs (siRNA) for selective suppression of protein production has rapidly become a commonly used technique for transient modulation of protein levels. In the present paper, we investigate whether introduction of platinated bases in the sense strand can be used to modulate the efficacy of siRNAs. Four different siRNAs were studied, all targeting the initial AU-rich 3' UT

TAS-20 alexithymia in obesity, and its links to personality

We studied the association between alexithymia (20-item Toronto Alexithymia Scale, TAS-20) and obesity, and also assessed the construct validity of the TAS-20 in terms of personality dimensions in obese patients. The TAS-20 and its subscales were analysed for their correlations with the NEO Personality Inventory - Revised (NEO PI-R) in an obese sample of 259 patients. Obesity was associated with h

Kinetics of the coupled reaction catalysed by a fusion protein of yeast mitochondrial malate dehydrogenase and citrate synthase: Kinetics of a fusion protein of MDH and CS

The mechanistic implications of the kinetic behaviour of a fusion protein of mitochondrial malate dehydrogenase and citrate synthase have been reanalysed in view of predictions based on experimentally determined kinetic parameter values for the dehydrogenase and synthase activities of the protein. The results show that the time-course of citrate formation from malate in the coupled reaction cataly

Sexual conflict over floral receptivity

In flowering plants, the onset and duration of female receptivity vary among species. In several species the receptive structures wilt upon pollination. Here we explore the hypothesis that postpollination wilting may be influenced by pollen and serve as a general means to secure paternity of the pollen donor at the expense of female fitness. Taking a game-theoretical approach, we examine the poten

Traffic-generated emissions of ultrafine particles from pavement-tire interface

In a road simulator study, a significant source of sub-micrometer fine particles produced by the road-tire interface was observed. Since the particle size distribution and source strength is dependent on the type of tire used, it is likely that these particles largely originate from the tires, and not the road pavement. The particles consisted most likely of mineral oils from the softening filler

Reliability of isokinetic ankle dorsiflexor strength measurements in healthy young men and women

The purposes of this study were: (i) to determine the test-retest reliability of isokinetic ankle dorsiflexor strength measurements in young healthy adults using the Biodex dynamometer, and (ii) to examine several statistical measures for the interpretation of reliability. Thirty men and women (mean age 23 +/- 3 years) performed three maximal concentric contractions at 30 degrees/s, 60 degrees/s,

Intracellular lipids and inflammatory activity in macrophage foam cells

Popular Abstract in Swedish Kardiovaskulär sjukdom karaktäriseras av lipid accumulering och inflammation i artärerna. Sjukdomen startar tidigt med att monocyter vandrar ut i kärlväggen där de differentierar till makrofager, tar upp modifierade lipoproteiner och utvecklas till skumceller. En samtidig inflammation bidrar till att fler celler och mer lipider ansamlas och ett aterosklerotiskt plack böCardiovascular disease (CVD) is characterized by lipid accumulation and inflammation in arteries. The disease starts early in life with migration of monocytes into the arterial wall where they engulf entrapped lipoproteins and become macrophage foam cell. A simultaneous inflammatory response further enhances lipid and cell accumulation at sites of lesion development, resulting in progression of at