The chapter provides a historic overview of the research in physics and chemistry for the development of radionuclides, radiopharmaceuticals, and instrumentation which have had a major impact for the today’s status of nuclear medicine. During the first half of the 20th century in particular, a number of researchers with relevance to the development of nuclear medicine have been awarded the Nobel P

Objective: To describe the clinical and ultrasound characteristics of three types of rare malignant ovarian germ cell tumor: embryonal carcinoma, non-gestational choriocarcinoma and malignant mixed germ cell tumor. Methods: This was a retrospective multicenter study. From the International Ovarian Tumor Analysis (IOTA) database, we identified patients with a histological diagnosis of ovarian embry

In this presentation we focus on men’s violence against the female population both during and in the wake of climate disasters in the Philippines and Vietnam. We examine the legal framework which has been ratified in the two countries to protect individual’s right to live a life without being abused and; how various types of organizations work to prevent and combat specific kinds of violence in th

Astrocytes are essential cells for normal brain functionality and have recently emerged as key players in many neurological diseases. However, the limited availability of human primary astrocytes for cell culture studies hinders our understanding of their physiology and precise role in disease development and progression. Here, we describe a detailed step-by-step protocol to rapidly and efficientl

Direct reprogramming is an emerging research field where you can generate neurons from a somatic cell, such as a skin or glial cell by overexpressing neurogenic transcription factors. This technique allows fast generation of subtype-specific and functional neurons from both human and mouse cells. Despite the fact that neurons have been successfully generated both in vitro and in vivo, a more exten

The detection of neurotransmitter release from reprogrammed human cell is an important demonstration of their functionality. Electrochemistry has the distinct advantages over alternative methods that it allows for the measuring of the analyte of interest at a high temporal resolution. This is necessary for fast events, such as neurotransmitter release and reuptake, which happen in the order of mil

Recent advances in genome editing have brought new hopes for personalized and precision medicine but have also dramatically facilitated disease modeling studies. Combined with reprogramming approaches, stem cells and differentiation toward neural lineages, genome engineering holds great potential for regenerative approaches and to model neurological disorders. The use of patient-specific induced p

Adult human cortical organotypic slice culture is an attractive model system to explore mechanisms of human brain pathology as well as to test drug candidates for treatment of neurodegeneration. Acute studies in human brain slices are limited by the lifetime of the tissue and focus mainly on hippocampus slice preparation. Here we describe the derivation of human organotypic slice cultures of corti

Introduction: Tumour related factors are well known as risk factors for survival after pancreatoduodenectomy (PD). In a time when the role for neoadjuvant treatment is discussed also in primary resectable patients, the influence of preoperative variables is of interest. The aim was to examine preoperative factors influence on early mortality following PD.Materials: Patients registered in the Swedi

Airborne emissions from disc brakes adversely affect the air quality in urban areas of EU. The emissions come from the wear of the pads and rotors in disc brakes. The wear of disc brakes depends on the contact situation between the pads and rotors. During braking, secondary contact plateaus are created on both the pads and rotors contact surfaces. Literature reports numerical studies of the creati