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In the present study, we have studied the expression of glial cell line-derived neurotrophic factor (GDNF) and its receptors Ret, GFRα1, and GFRα2 in the retino-tectal system before and after optic nerve transection. Using retrograde neuronal tracing in combination with in situ hybridization, we found that Ret and GFRα1 are expressed by 13-14% of the retinal ganglion cells (RGCs). These Ret-expres
The aim of this study was to characterise the expression of the melanocortin system in the normal and injured rat visual system. Using real-time polymerase chain reaction and immunohistochemistry, we detected melanocortin MC3, MC4 and MC5 receptors and proopiomelanocortin in adult retina and superior colliculus. Melanocortin MC4 receptor mRNA was the most abundant receptor. Melanocortin MC3, MC4 a
Increased expression of Bim, a pro-apoptotic member of the Bcl-2 family, has been shown to be critical for neuronal apoptosis. To study the involvement of Bim in injury-induced cell death in retina, Bim expression was studied in normal rat retina and in retina after optic nerve transection using quantitative RT-PCR and immunohistochemistry. As a complement to this, the apoptotic regulators Bax, Bc
We describe a protocol for analysis of gene expression in single, acutely dissociated adult rat retinal ganglion cells using RT-PCR. Retrograde tracing of retinal ganglion cells from the superior colliculi was conducted using Fluorogold. Retinas were dissected and ganglion cells isolated using retinal layer separation (sandwiching). Single, fluorescently labelled retinal ganglion cells were aspira
GDNF and the GDNF receptors, c-Ret, GFRα1 and 2 mRNA is expressed in the developing chicken retina. GDNF labelling was mainly found in embryonic day 4-5 retina but weak labelling could also be found over scattered retinal cells at later stages. c-ret labelling was found over ganglion cells, amacrine and horizontal cells; the preferred GDNF receptor (GFRα1) over amacrine and horizontal cells; and t
Objectives: The purpose of this presentation is to discuss Sweden’s national recommendations for mental health treatment provided to self-harming individuals in the country. The recommendations were based upon a Self-Harm Quality Standard (2013) published by the National Institute for Health and Care Excellence (NICE) adapted to Swedish health care and cultural context. Methods: A review and synt
Objectives: To measure treatment fidelity and to evaluate patient and clinician experiences with Brief Admission Skåne (BAS) over the pilot phase and first five months of the intervention phase of a randomized controlled trial (RCT) testing BAS. The purpose of the RCT is to determine whether BAS can reduce the frequency of acute psychiatric admissions for individuals with borderline personality di
Modelling tools used in impact assessment procedures can be regarded as tools for communication between science and policy. In order to create an integrated system for modelling not only the scientific components have to be in place but also the science/policy interfaces in the assessment procedures have to be identified and their social dynamics understood.To make a system like SEAMLESS Integrate
