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HvPIP1;6, a barley (Hordeum vulgare L.) plasma membrane water channel particularly expressed in growing compared with non-growing leaf tissues

The aim of the present study was to identify water channel(s) which are expressed specifically in the growth zone of grass leaves and may facilitate growth-associated water uptake into cells. Previously, a gene had been described (HvEmip) which encodes a membrane intrinsic protein (MIP) and which is particularly expressed in the base I cm of barley primary leaves. The functionality of the encoding

The function of alpha-crystalline emulsifiers on expanding foam surfaces

The expanding capacity and the stabilizing function of an alpha-crystalline emulsifier on the bubble surfaces during and after expansion of a sugar foam were examined by volume measurements, confocal laser scanning microscopy (CLSM), freeze-etching and transmission electron microscopy (TEM) and oscillatory theological measurements. 0.2-10% (w/w) emulsifier, either a polyglycerol ester mixed with m

Oxalate- and Squarate-Biimidazole Supramolecular Synthons: Hydrogen-Bonded Networks Based on [Co(H(2)biimidazole)(3)](3+)

The utility of R-2(2)(9) biimidazole-carboxylate, R-2(2)(10) biimidazole-oxalate/squarate and R-2(2) (9) biimidazole-(tris-oxalate) synthons is considered for crystal engineering of hydrogen-bonded networks based on [Co-III(H(2)biim)(3)](3+) cations (H(2)biim = 2,2-biimidazole) and oxalate, squarate or [M-III(C2O4)(3-) anions. Syntheses and crystal structures are described for [Co-III(H(2)biim)(3)

A course of instruction for women with irritable bowel syndrome.

Objective: The aim of this study was to determine the effects a course of instruction about irritable bowel syndrome (IBS) might have on symptoms, psychological well-being, and medical care requirements in these patients. Methods: Twenty-nine women with IBS participated in a programme of instruction on medical care, physical activity, stress-management, diet and health insurance. The women complet

Co-ordination of spontaneous swallowing with respiratory airflow and diaphragmatic and abdominal muscle activity in healthy adult humans

Co-ordination of breathing and swallowing is essential for normal pharyngeal function and to protect the airway. To allow for safe passage of a bolus through the pharynx, respiration is interrupted (swallowing apnoea); however, the control of airflow and diaphragmatic activity during swallowing and swallowing apnoea are not fully understood. Here, we validated a new airflow discriminator for detec

Metaphase cytogenetics and DNA flow cytometry with analysis of S-phase fraction in prostate cancer: influence on prognosis

OBJECTIVES: To compare the prognostic significance of chromosome aberrations, DNA ploidy, and S-phase fraction (SPF) in prostate adenocarcinomas and to compare the sensitivity of metaphase cytogenetics with flow cytometry (FCM) in detecting abnormal tumor clones. METHODS: Prostate adenocarcinomas from 57 men were previously successfully analyzed with metaphase cytogenetics. Archival material from

Human monoclonal antibodies with different fine-specificity for digoxin derivatives: Cloning of heavy and light chain variable region sequences

Human-mouse hybridoma cell lines producing human monoclonal antibodies against the cardiac glycoside digoxin were established after in vitro immunization or direct immortalization of human peripheral blood lymphocytes with digoxin. Three antibodies, designated M06, LH92 and LH 1 14, displayed different patterns of fine specificity against digoxin and several digoxin analogues, as elucidated by inh

BCL11B participates in the activation of IL2 gene expression in CD4+ T lymphocytes

BCL11A and BCL11B are transcriptional regulators important for lymphopoiesis and previously associated with hematopoietic malignancies. Ablation of the mouse Bcl11b locus results in failure to generate double-positive thymocytes, implicating a critical role of Bcl11b in T-cell development. However, BCL11B is also expressed in CD4+ T lymphocytes, both in resting and activated states. Here we show b

Serotonin uptake into cerebrovascular nerve fibers of rat, visualization by immunohistochemistry, disappearance following sympathectomy, and release during electrical stimulation

Immunohistochemistry as well as in vitro uptake and release of [3H]5-HT were performed on pial arteries of rat to investigate the nature of 5-HT containing nerve fibers. Immunoreactive fibers were constantly found only in the basilar, vertebral and superior cerebellar arteries, while in the other parts of the circle of Willis, 5-HT immunofluorescent fibers were absent. After systemic treatment wit

Nurses' experiences of the encounter with elderly patients in acute confusional state in orthopaedic care.

The aim of this study was to illuminate nurses’ experiences of the encounter with elderly patients who developed acute confusional state (ACS) in orthopaedic care. Forty-eight nurses with professional background as registered (n=26) or licensed practical nurses (n=22) who took part in the nursing care of acute confused patients were involved. Open-ended unstructured interviews were conducted with

Accuracy of Kinematic and Dynamic Models of a Gantry-Tau Parallel Kinematic Robot

In this article, a new kinematic and a dynamic model for a 3-degree-of-freedom Gantry-Tau parallel kinematic robot are presented. Similar to an earlier proposed kinematic model, the dynamic model is based on the assumption of parallel actuator axes and constant end-effector orientation. The new kinematic model takes into account rotations of actuator axes which do not affect the end-effector orien

Feasibility and linguistic validity of the Swedish version of the PDQ-39

The Parkinson’s Disease Questionnaire – 39 is the most widely used disease-specific health status questionnaire for Parkinson’s disease. Evaluations of various language versions have focused on psychometric properties, whereas documented evaluations regarding linguistic validity, respondent burden and questionnaire content are largely lacking. Work with the Swedish Parkinson’s Disease Questionnair

A new and evolving paradigm for biocompatibility

We propose that the mechanical property of the interface between an implant and its surrounding tissues is critical for the host response and the performance of the device. The interfacial mechanics depends on several different factors related to the physical shape of the device and its surface as well as properties of the host tissue and the loading conditions of the device and surrounding tissue

TXNIP regulates peripheral glucose metabolism in humans

Background Type 2 diabetes mellitus ( T2DM) is characterized by defects in insulin secretion and action. Impaired glucose uptake in skeletal muscle is believed to be one of the earliest features in the natural history of T2DM, although underlying mechanisms remain obscure. Methods and Findings We combined human insulin/glucose clamp physiological studies with genome-wide expression profiling to id

A new cyclodextrin glycosyltransferase from an alkaliphilic Bacillus agaradhaerens isolate: purification and characterisation

A new, cyclodextrin glycosyltransferase (CGTase) from an alkaliphilic B. agaradhaerens strain LS-3C, isolated front an Ethiopian soda lake. was purified up to 43-fold by starch adsorption with a yield of 50%. The enzyme was a monomer with an estimated molecular weight of 110 kDa, representing the largest Bacillus CGTase reported so far. The isoelectric point (pI) of the enzyme was 6.9. The CGTase

Atrophy and neoplastic transformation of the ileal pouch mucosa in patients with ulcerative colitis and primary sclerosing cholangitis - A case control study

INTRODUCTION: Patients with ulcerative colitis and primary sclerosing cholangitis have an increased risk of developing carcinoma both in the bile ducts and in the colon. PURPOSE: To investigate whether this patient group also has an increased risk of developing atrophy and neoplasia in the deal pouch mucosa after construction of a pelvic pouch with an ileoanal anastomosis or a continent Kock ileos