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The growing field of biotechnology is in constant need of binding proteins with novel properties. Not just binding specificities and affinities but also structural stability and productivity are important characteristics for the purpose of large-scale applications. In order to find such molecules, libraries are created by diversifying naturally occurring binding proteins, which in those cases serv
The objective of this study was to investigate B-lymphocyte reconstitution in patients undergoing allogeneic haematopoietic stem cell transplantation (HSCT) after myeloablative conditioning (MAC) or reduced-intensity conditioning (RIC) regimens. B-lymphocyte reconstitution was studied by monitoring the CDR3 repertoire with spectratyping. We demonstrate a delay in the recovery of the B-lymphocyte r
Purified polyclonal immunoglobulin preparations representing the 4 rat IgG subclasses were tested for binding to Staphylococcus aureus protein A attached to 3 different solid phases (Staphylococcus aureus Cowan I bacteria, Sepharose CL4B and Sepharose 6MB). Protein A Sepharose CL4B showed higher reactivity with IgG1 and IgG2b than the staphylococci, whereas protein A Sepharose 6MB showed a lower u
The paper discusses dynamic real-time scheduling in the context ofmodel predictive control (MPC). Dynamic scheduling in this settingis motivated by the highly varying execution times associated withMPC controllers. Premature termination of the optimization algorithmis exploited to trade off prolonged computations versuscomputational delay. A feedback scheduling strategy for multiple MPCcontrollers
Periodontitis is an inflammatory disease of the supporting structures of the teeth and is caused by, among other agents, Porphyromonas gingivalis. P. gingivalis is very resistant to killing by human complement, which is present in a gingival fluid at 70% of the serum concentration. We found that the incubation of human serum with purified cysteine proteases of P. gingivalis (gingipains) or P. ging
Protein G, a bacterial cell wall protein extracted from strains of Streptococci, has been employed as a ligand in high performance liquid affinity chromatography (HPLAC) for separation of monoclonal antibodies. Examples are given of rapid high-resolution separations of rat and mouse monoclonal antibodies belonging to various subclasses. In comparison with protein A chromatography, we were able to
We investigate the origin of close-in planets and related phenomena orbiting white dwarfs (WDs), which are thought to originate from orbits more distant from the star. We use the planetary architectures of the 75 multiple-planet systems (four, five, and six planets) detected orbiting main-sequence stars to build 750 dynamically analogous templates that we evolve to the WD phase. Our exploration of
This article describes and critically examines the challenging task of compiling The London–Lund Corpus 2 (LLC–2) from start to end, accounting for the methodological decisions made in each stage and highlighting the innovations. LLC–2 is a half-a-million-word corpus of contemporary spoken British English with recordings from 2014 to 2019. Its size and design are the same as those of the world's f
Proteomics Data Collection (ProDaC) is an EU-funded Coordination Action within the 6th framework programme. It aims to simplify the publication, dissemination and utilization of proteomics data by establishing standards that will support broad data collection from the research community. As a part of ProDaC, regular workshops are organized on a half-yearly basis to enable communication and discuss
The retail sector is facing fundamental transformations in nearly every part of the value chain, affecting the need for competence development in many retail professions. As most learning at work is informal and there is a deficit of studies of how such learning takes place in specific professions, systematized work with competence development is hampered. We therefore investigated the informal le
OBJECTIVE: To clarify, using an extended adoption design, the sources of parent-offspring transmission for anxiety disorder (AD) and its major subforms and their familial cross-generational relationship with major depression (MD). METHODS: Offspring (born 1960-1992) and their parents, from six family types (intact, not-lived-with biological father or mother, lived-with step-father or step-mother,
Background: Harmful glucose exposure and absorption remain major limitations of peritoneal dialysis. We previously showed that inhibition of sodium glucose cotransporter 2 did not affect glucose transport during peritoneal dialysis in rats. However, more recently we found that phlorizin, a dual blocker of sodium glucose co-transporter 1 and 2, reduces glucose diffusion in peritoneal dialysis. Ther
Edinburgh was an Enlightenment city of regional, national and global influence. But how did the people of Enlightenment Edinburgh understand and order their world? How did they encounter, compare and produce different kinds of spaces, from the urban to the world scale? And how did this city set the universal standards by which other places should be judged and transformed?The Geographies of Enligh
Due to an increased number of complex multi- and long-term ill patients, healthcare and nursing provided in patients' homes are expected to grow. Teamwork is important in order to provide effective and safe care. As care becomes more complex, the need for teamwork in home care nursing increases. However, the literature on teamwork in the patients' home environment is limited. The aim of this study
Institutet strafföreläggande införs i svensk rätt år 1948. Syftet med systemet är att effektivt kunna lagföra lindriga och enkla brott, till exempel ringa stöld och olovlig körning. Brottmålsförfarandet tillåter den misstänkte att godkänna ett av åklagaren utfärdat strafföreläggande som avser en påföljd för ett begånget brott. Om den anklagade accepterar strafföreläggandet avstår han eller hon fråThe summary imposition of a fine is introduced into Swedish law in 1948. The purpose of the system is to take legal proceedings more efficiently against minor and simple criminal cases, such as petty theft and unlawful driving. The criminal procedure allows the suspect to approve an injunction filed by the prosecutor regarding a punishment for a committed crime. If the accused accepts the injuncti
Changes to the Education Act in 2025 will mean that school libraries will have a specific objective of promoting students' reading and media and information literacy. Meanwhile, school library spaces can vary greatly in terms of accessibility and size. The aim of this thesis is therefore to provide knowledge about how the work of school librarians is affected by the possibilities and limitatio
In this essay, I will examine what Origen of Alexandria believed about the resurrection of the body. Origen was condemned as a heretic at the Second Council of Constantinople in 553, one of the main reasons for this was that he was said to deny the physical resurrection of the body. Due to the condemnation of Origen, the work of copying his works was also stopped, with the result that many of Orig
In this work, we explore Higgs boson decays into dark matter (DM) within the context of the freeze-in mechanism under stronger coupling scenarios. Our analysis focuses on scalar DM candidates interacting through the Higgs portal, where the coupling strength can be significant without establishing thermal equilibrium, owing to a suppressed reheating temperature. We find that for DM masses as low as
Införandet av LAS-åldern i 32 a § lagen (1982:71) om anställningsskydd har etablerat en lagstadgad rätt för arbetstagare att kvarstå i arbete till 69 års ålder. Trots detta förekommer det i vissa kollektivavtal bestämmelser om åldersgränser som understiger LAS-åldern. Sådana be- stämmelser motiveras ofta av säkerhetskrav, fysiska förutsättningar eller socialpolitiska ända- mål. Syftet med uppsatseThe implementation of the LAS-age in 32 a § the Swedish Employment Protection Act (Lag (1982:71) om Anställningsskydd) has established a legal right for the employee to continue working up to 69 years of age. Despite this, certain collective agreements contain provisions on age-based mandatory resignation at a lower age. Such provisions are often justified by con- siderations relating to safety, p
