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Urban underwater ERT for site investigation in lake Mälaren, Sweden

An underwater ERT survey was made along a planned road tunnel for The Stockholm Bypass Project (Förbifart Stockholm). The aim was to assess the ability to identify variations in the depth of the bottom sediments, as well as variations in rock quality and the possible presence of weak zones in the rock. Reference data from a seismic survey and geotechnical drilling was used for evaluation the resul

Identification of Cys255 in HIF-1α as a novel site for development of covalent inhibitors of HIF-1α/ARNT PasB domain protein-protein interaction

The heterodimer HIF-1α (hypoxia inducible factor)/HIF-β (also known as ARNT-aryl hydrocarbon nuclear translocator) is a key mediator of cellular response to hypoxia. The interaction between these monomer units can be modified by the action of small molecules in the binding interface between their C-terminal heterodimerization (PasB) domains. Taking advantage of the presence of several cysteine res

Observations made on the museums in Portici and on the Vesuvian sites by two Swedish professionals in 1756 and 1768, respectively

This article presents two formerly unedited travelaccounts, transcribed from the Swedish manuscripts and translated into English, narrating visits to Herculaneum, the Herculanense Museum and Pompeii, by Georg Fröman, Royal maçon, and Johan Tobias Sergel, sculptor, respectively. The texts are commented and put in historical context. Sergel's thorough accout of the Museum is read in parallel with th

An integrated approach to design site specific distributed electrical hubs combining optimization, multi-criterion assessment and decision making

An integrated approach is presented in this study to design electrical hubs combining optimization, multi-criterion assessment and decision making. Levelized Energy Cost (LEC), Initial Capital Cost (ICC), Grid Integration Level (GI), Levelized CO2 emission (LCO2), utilization of renewable energy, flexibility of the system, loss of load probability (LOLP) are considered as criteria used to assess t

Post-socialist moral economies of consumption as socially embedded sites of moral tension

Consumption was one of the spheres of everyday life shaken and remoulded by the collapse of socialist regimes at the end of the 20th century. Especially in times of rapid change, a moral economies lens is a fruitful means to understand the contentious moral landscape and moral tensions that pervade consumption behaviour, norms and discourse. Important areas of consideration in post-socialist socie

A transformation of the Memorial Site in Katyn

In the wake of the deterioration of relations between modern Russia and Poland, the Katyn memorial has become a scene for the contestation of historical memory. In order to play down the 1940 executions of Polish military officers in Katyn, the Russian government has granted belated official recognition to 8,000 victims of Stalinism in the Smolensk region. After being brushed under the carpet in t

AfsK-Mediated Site-Specific Phosphorylation Regulates DnaA Initiator Protein Activity in Streptomyces coelicolor

In all organisms, chromosome replication is regulated mainly at the initiation step. Most of the knowledge about the mechanisms that regulate replication initiation in bacteria has come from studies on rod-shaped bacteria, such as Escherichia coli and Bacillus subtilisStreptomyces is a bacterial genus that is characterized by distinctive features and a complex life cycle that shares some propertie

A bispecific IgG format containing four independent antigen binding sites

Bispecific antibodies come in many different formats, including the particularly interesting two-in-one antibodies, where one conventional IgG binds two different antigens. The IgG format allows these antibodies to mediate Fc-related functionality, and their wild-type structure ensures low immunogenicity and enables standard methods to be used for development. It is however difficult, time-consumi

Contributions of dust and biomass burning to aerosols at a Colorado mountain-top site

Visible Multifilter Rotating Shadowband Radiometer (vis-MFRSR) data were collected at Storm Peak Laboratory (SPL), a mountain-top facility in northwest Colorado, from 1999 to 2011 and in 2013. From 2011 to 2014, in situ measurements of aerosol light scattering were also obtained. Using these data sets together, the seasonal impact of dust and biomass burning is considered for the western USA. Anal

No particle mass enhancement from induced atmospheric ageing at a rural site in northern Europe

A large portion of atmospheric aerosol particles consists of secondary material produced by oxidation reactions. The relative importance of secondary organic aerosol (SOA) can increase with improved emission regulations. A relatively simple way to study potential particle formation in the atmosphere is by using oxidation flow reactors (OFRs) which simulate atmospheric ageing. Here we report on the

Sequence and context dependence of EF-hand loop dynamics. An 15N relaxation study of a calcium-binding site mutant of calbindin D(9k)

The influence of amino acid sequence and structural context on the backbone dynamics of EF-hand calcium-binding loops was investigated using 15N spin relaxation measurements on the calcium-free state of the calbindin D(9k) mutant (A14D+A15Δ+P20Δ+N21G+P43M), in which the N-terminal pseudo- EF-hand loop, characteristic of S100 proteins, was engineered so as to conform with the C-terminal consensus E