Native And Chimeric Metal-Binding Lactate Dehydrogenase As Detection And Protection Tools For Oxidative Stress Induced By Fenton'S Reaction
In the present study, a simple and reliable antioxidant screening technique based on lactate dehydrogenase (LDH) oxidation by Cu2+-mediated Fenton's reaction has successfully been developed. Oxidation of LDH by hydroxyl radical consequently leads to enzymatic inactivation, while addition of antioxidants can protect and regain enzyme activity. This method demonstrated a high feasibility on detectin
