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The ultrastructure of the striated muscles of Derocheilocaris typica (Mystacocarida, Crustacea).

The striated muscles of Derocheilocaris typica consist of mononucleated cells, each containing one filament bundle. Large muscles consist of two or more cells adjacent to each other. The mitochondria line up along the filament bundle on one side. The nucleus is situated in the mitochondrial row and has a small cytoplasmic area around it filled with glycogen. The sarcomeres are between 3 and 6 μm l

Localized Surface Plasmon Resonance Sensing of Lipid-Membrane-Mediated Biorecognition Events

Supported phospholipid bilayers (SPBs) have emerged as important model systems for studies of the natural cell membrane and its components, which are essential for the integrity and function of cells in all living organisms, and also constitute common targets for therapeutic drugs and in disease diagnosis. However, the preferential occurrence of spontaneous SPB formation on silicon-based substrate

Two-step recovery process for tryptophan tagged cutinase: Interfacing aqueous two-phase extraction and hydrophobic interaction chromatography

In this work, the interfacing of a poly(ethylene glycol) (PEG)-phosphate aqueous two-phase system with hydrophobic interaction chromatography (HIC) for primary recovery of an intracellular protein was evaluated. As a model protein, a recombinant cutinase furnished with a tryptophan-proline (WP) peptide tag was used and produced intracellularly in Escherichia coli (E. coli). E. coli cell homogenate

131I-labelled anti-CD22 MAb (LL2) in patients with B-cell lymphomas failing chemotherapy. Treatment outcome, haematological toxicity and bone marrow absorbed dose estimates.

The experience with radioimmunotherapy in B-cell lymphomas using the rapidly internalizing antibody, anti-CD22 (LL2), is limited. In this study we investigated the efficacy and toxicity of 131I-labelled-LL2 for radioimmunotherapy in patients with B-cell lymphomas that failed one or two cytostatic regimens. Eleven patients were treated with one or repeated cycles of 131I-anti-CD22 antibody, 1330 MB

Growth and characterization of defect free GaAs nanowires

Most III-V compound semiconductor nanowires seeded by metal particles grow preferentially in a (over bar 1 over bar 1 over bar 1)B direction (B wires) and most commonly with many stacking faults perpendicular to the growth direction. If growth proceeds in an alternate direction, defect-free growth has been observed. We present experimental results for the growth of GaAs nanowires in a previously u

A highly sensitive flow-through amperometric immunosensor based on the peroxidase chip and enzyme-channeling principle

A concept based on the Peroxidase-chip (P-chip), antibody co-immobilization, competitive and enzyme-channeling principle was exploited to develop an integrated flow-through amperometric biosensor for detection of environmental pollutants such as s-triazine herbicides. In this concept, recombinant peroxidase is immobilized on the gold electrode (P-chip) in such a way that direct electron transfer i

Graded effects of oxygen and respiratory inhibitors on cell metabolism and spontaneous contractions in smooth muscle of the rat portal vein

The basis for the hypoxic relaxation of spontaneous activity in the rat portal vein was investigated by comparing responses to oxygen and the respiratory chain inhibitors amobarbital and cyanide. With the inhibitors, O2 consumption (FO2) is uniformly decreased throughout the cell mass, and thus O2 gradients in the tissue are avoided. Hence the effects are not to be attributed to all-or-none inhibi

The practice of internal medicine in Europe: organisation, clinical conditions and procedures

Background: Current information on the role of internists in the European countries is scarce. This report describes the results of a survey of the practice of internists in Europe. Methods: Two online questionnaire-based surveys were carried out by the European Board of Internal Medicine, one on the practice of internists and the other on postgraduate training in internal medicine. The national i

Impact of bilateral cataract surgery on vision-related activity limitations

PURPOSE: To evaluate patients' vision-related activity limitation before and after bilateral cataract surgery using the German version of the Catquest-9SF questionnaire. SETTING: Hanusch Hospital, Vienna, Austria, and Ophthalmic Clinic, Minden, Germany. DESIGN: Questionnaire development. METHODS: This prospective 2-center study included patients who were scheduled for bilateral cataract surgery an

Familial risk of sleep-disordered breathing

Objective: To estimate the incidence of hospitalization for paediatric obstructive sleep apnoea syndrome (OSAS) or sleep-disordered breathing (SDB) caused by adenotonsillar or tonsillar hypertrophy without infection in children with a parent affected by OSAS. Patients and methods: Using the MigMed database at Lund University, hospital data on all children aged 0-18 years in Sweden between 1997 and

Diffusive-ballistic heat transport in thin films using energy conserving dissipative particle dynamics

Diffusive-ballistic heat transport in thin films was simulated using energy conserving dissipative particle dynamics (DPDe). The solution domain was considered to be two-dimensional with DPD particles distributed uniformly under constant temperature boundary conditions at the top and bottom walls and periodic boundaries at the side walls. The effects of phonon mean free path were incorporated by i

Thrombus Aspiration in ST-Elevation myocardial infarction in Scandinavia (TASTE trial). A multicenter, prospective, randomized, controlled clinical registry trial based on the Swedish angiography and angioplasty registry (SCAAR) platform. Study design and rationale

Background In ST-elevation myocardial infarction (STEMI), distal embolization of thrombus material often precludes restoration of normal coronary artery flow. Small-scaled studies have demonstrated that intracoronary thrombus aspiration improves flow and myocardial perfusion, but only one larger randomized single-center study has suggested a survival benefit. Thrombus aspiration is widely used in

In vitro Transcription/Translation System: A Versatile Tool in the Search for Missing Proteins

Approximately 18% of all human genes purported to encode proteins have not been directly evidenced at the protein level, according to the validation criteria established by neXtProt, and are considered as “missing” proteins. One of the goals of the Chromosome-Centric Human Proteome Project (C-HPP) is to identify as many of these “missing” proteins as possible in human samples using mass spectromet

Passive knee muscle moment arms measured in vivo with MRI

OBJECTIVE: To determine moment arm lengths from seven knee muscles and the patellar tendon. The knee muscles were the biceps femoris, semitendinosus, semimembranosus, gracilis, sartorius, and the lateral and medial gastrocnemius muscles. DESIGN: The moment arms were calculated based on MRI measurements. BACKGROUND: Moment arm lengths of different muscles with respect to the joint centre of rotatio

The object markers ba and jiang in modern literary Chinese

Popular Abstract in English Modern standard written Chinese has two common object markers, 把 ba and 将 jiang, both of which mark a direct object preceding the verb. Both markers appear to have been widely used in the spoken language of the Tang dynasty but the actual distribution is not entirely clear. Today jiang has disappeared from the dialects and subdialects of northern China and it is mainly Modern standard written Chinese has two common object markers, 把 ba and 将 jiang, both of which mark a direct object preceding the verb. Both markers appear to have been widely used in the spoken language of the Tang dynasty but the actual distribution is not entirely clear. Today jiang has disappeared from the dialects and subdialects of northern China and it is mainly used in the Yue dialect (Can

Female Counter-Culture and Catholic Mission: The Sister of Saint Joseph in Denmark and Sweden

This article is dealing with the work of a Catholic St Joseph Sisters in the Nordic countries, particularly in Denmark and Sweden, from the sisters’ arrival in 1856 to the 1920:ies. These Catholic sisters belonged to a French congregation called La Congregation des Sœurs de Saint-Joseph de Chambéry, which was founded at the beginning of the nineteenth century and whose motherhouse was in Chambéry

Keeping My Ways of Being. Middle-aged women and menopause

Popular Abstract in Swedish Att fortsätta leva på mitt sätt. Medelålders kvinnor och menopaus/klimakteriet. Varje år passerar cirka 65 000 svenska kvinnor sin menopaus. Menopaus är en medicinsk term och avser tidpunkten för en kvinnas sista menstruation. Tidpunkten för menopaus kan därför i regel endast avgöras i efterhand, vanligtvis först efter ett års uppehåll av de naturliga menstruationernaTo deepen the understanding of menopause and its role in women's lives is the general aim of this thesis. Between 1995 and 2003, all women in two Swedish primary health care districts received a postal questionnaire, when they were 45, 50, 55 or 60. A total of 1681 women (76%) responded. Twenty-four women were given research interviews. The effects of menopause on quality of life (QoL) and number

The importance of sexual and asexual reproduction in the recent evolution of Allium vineale

In the weedy plant species Allium vineale (wild garlic), individuals may simultaneously produce sexually and asexually derived offspring, by seed and bulbils, respectively. In this study, genetic and genotypic diversity was determined in samples from 14 European A. vineale populations using nuclear (RAPD) and cytoplasmic (PCR-RFLP of cpDNA) markers to investigate the importance of the different re

Regional brain serotonin and dopamine transporter binding capacity in suicide attempters relate to impulsiveness and mental energy.

To study different aspects of regional pre-synaptic brain 123I-β-CIT uptake on serotonin and dopamine re-uptake sites in drug-free suicide attempters in comparison with age- and sex matched control subjects, single photon emission computed tomography (SPECT) measurements were analysed for regional serotonin re-uptake (5HTT) and dopamine re-uptake (DAT) capacity (binding potential, BPlow asterisk)