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Nucleotide metabolism and replication of nucleic acids are processes of fundamental importance. A crucial enzyme in nucleotide metabolism is dUTPase (E.C. 3.6.1.23), that catalyses the hydrolysis of dUTP to dUMP and pyrophosphate. The activity of the enzyme has influence on the outcome of DNA replication and complete loss of enzyme activity is lethal, while a decreased activity promotes increased

The setting for this study is Swedish film culture of the 1920s, which has been studied with a focus on representations of masculinity and gender relations according to four themes: 1) children and youth 2) fatherhood and love 3) sexuality and popularity 4) ethnicity and racial stereotyping. The rise of new consumer culture in the first decades of the 20th century created turmoil between traditio

Popular Abstract in Swedish Den molekylära processen under vilken DNA-information förs över till RNA-information kallas transkription. Transkription styrs främst i samverkan med många speciella molekyler som kallas transkriptionsfaktorer. Zinkfinger proteiner är en av de vanligast förekommande klasserna av reglerande proteiner i eukaryota celler där de inverkar på en mängd av cellulära aktiviteterThe molecular process under which message in DNA is relayed onto RNA is called transcription, and it accomplished through involvement of so-called transcription factors. Zinc finger proteins are one of the most abundant classes of regulatory proteins in eukaryotic cells where they play central roles in a variety of cellular activities. A classical zinc finger is a small protein motif with a simple

This thesis describes the design and characterisation of a vacuum ultraviolet scanning photoelectron microscope as well as results from studies of laterally inhomogeneous surfaces. The instrument utilises synchrotron radiation from an undulator at the 550 MeV storage ring at the MAX-laboratory. The beamline is comprised of a plane-grating monochromator with a Kirkpatrick-Baez objective and a graz

Ebola virus (EBOV) belongs to the group of nonsegmented negative-sense RNA viruses. The seven EBOV genes are separated by variable gene borders, including short (4- or 5-nucleotide) intergenic regions (IRs), a single long (144-nucleotide) IR, and gene overlaps, where the neighboring gene end and start signals share five conserved nucleotides. The unique structure of the gene overlaps and the prese

The object of this thesis is to describe, analyze and understand the terms and meanings of the public information and the political communication for local democracy, in today´s media landscape, out of the interaction between the main parties of the local society in Sjöbo and Ystad and from the stand point of earlier research and theories. The study has analyzed the actions performed by citizens,

Popular Abstract in Swedish Ta ett djupt andetag och tänk på vad du nu har i lungorna! Jo, det är luft, förstås, syre kväve och sådant. Men det är också något annat. I varje kubikcentimeter du andas in finns tusentals partiklar, alla för små för att kunna ses med blotta ögat. Om dessa partiklar handlar denna avhandling. Vi kallar luftburna partiklar för aerosoler, ett begrepp som innefattar allt Airborne particles are everywhere around us, and have always been. Particles generated by human activities has increased drastically since industrialization, and several epidemiological studies have shown that inhaled particles can cause adverse health effects. The concern about health effects have during the last decade shifted towards fine and ultrafine particles, not least due to the emerging f

Popular Abstract in Swedish Vår värld drivs av förbränning. Från den lilla elden som används vid matlagning till gigantiska gasturbiner och kolkraftverk som genererar en stor del av all elektricitet. Dessutom så drivs nästan alla fordon av förbränningsmotorer. Det har uppskattats att över nittio procent av alla energi vi i världen omvandlar kommer från förbränning i någon form. Det är således ganHigh repetition rate laser diagnostics has proven to be a useful tool for studying combustion phenomena. Laser techniques are, in general, relatively non-intrusive and have the potential to measure specific species at very low concentrations and with extremely high temporal resolution. However, most laser systems in this field of research are limited to repetition rates below 100 Hz, and are thus

Purpose: The detection of malignant tumours relies on a variety of diagnostic procedures including X-ray images and, for hollow organs, endoscopy. The purpose of this study was to present a new technique for non-invasive tumour detection based on tissue fluorescence imaging. Material and Methods: A clinically adapted multi-colour fluorescence system was employed in the real-time imaging of maligna

Popular AbstractSyftet med avhandlingen är att undersöka på vilket sätt teater med ungdomar, enligt ungdomarnas egna erfarenheter och utsagor om det, utgör en kollektiv bildningsprocess. I avhandlingens syfte ingår att analysera hur en teaterföreställning växer fram och att skapa förståelse för vad som sker mellan olika aktörer, främst mellan ungdomar och ledare.Forskningsfenomenet, unga människorThe objective of this research project is to investigate in what way young people's theatre playing, according to their own experiences, constitute a collective educational process. Also, the project aims at understanding the interaction between the different actors, and particularly between the young amateurs and their leaders. Young people's acting in this research project is understood from a p

The Öresund Region is a transnational region encompassing areas of southern Sweden (Skåne) and southeast Denmark (Sjælland and Hovedstaden). The Energi Öresund project seeks to offer strategic and cooperative energy planning for the region as a whole in order to meet the energy goals of Sweden, Denmark, and its constituent local municipalities. The project is funded by EU INTERREG IV, a European U

Popular Abstract in Swedish Antikroppar är viktiga beståndsdelar av det naturliga immunförsvaret som skyddar oss mot smittämnen, såsom bakterier och virus. Utvecklingen av olika genetiska och biokemiska teknologier har dessutom möjliggjort användningen av antikroppar för en mängd ändmål, bl a för diagnostik och behandling av infektionssjukdomar och cancer. I denna avhandling, som bygger på fem vetAntibodies are key players of the immune system in higher vertebrates, which provide a defense against potentially lethal threats from the environment. Besides their importance in the immune defense, antibodies have a great potential as reagents in biological chemistry and diagnostics, and as therapeutic agents against both infectious diseases and cancer. Despite tremendous advances in all fields

Two-photon-induced Degenerate Four-Wave Mixing, DFWM, was applied for the first time to the detection of CO, and NH3 molecules. Measurements were performed in a cell, and in atmospheric-pressure flames. In the cell measurements, the signal dependence on the pressure and on the laser beam intensity was studied. The possibility of simultaneous detection of NH3 and OH was investigated. Carbon monoxid

The aim of the work presented in this thesis was to investigate nature of adsorption of amphiphiles to the solid–liquid interface. The surface aggregate structures of the zwitterionic surfactant dodecyldimethylammoniopropanesulfonate, (DDAPS) and a series of nonionic polyoxyethlylene glycol alkyl ethers (C10E5, C12E5, C12E8, C10E6, C14E6 and C16E6) have been determined by Atomic Force Microscopy o

Popular Abstract in Swedish POPULÄRVETENSKAPLIG SAMMANFATTNING PÅ SVENSKA Vår arvsmassa finn's lagrad som linjära dubbelsträngade DNA-molekyler, kromosomer, i våra celler. Celler i vävnader och organ förnya's ständigt genom celldelning. Kromosomerna i en cell måste replikera's för att kunna ge upphov till två nya celler. Replikeringsmaskineriet har bara förmågan att arbeta i en riktning läng's meABSTRACT Telomerase activity is a potential molecular marker for cancer and primitive cells in regenerative tissues. The most commonly used method to measure telomerase activity in cells is the telomerase reverse amplification protocol (TRAP) assay, where the protein extracts derived from homogenized cells are analysed. In this thesis we focused on developing a method where telomerase activity co

The WT1 protein, a product of the Wilms’ tumour 1 gene (WT1) is a zinc finger transcription factor implicated in a number of cellular processes particularly involved in the development of the urogenital system. Mutations in this gene have been implicated in abnormal development of the urogenital system resulting in syndromes such as the Denys-Drash, WAGR and Frasier and in the childhood kidney mal

In semiarid and arid Tunisia, water quality and agricultural practices are the major contributing factors to the degradation of soil resources threatening the sustainability of irrigation systems and agricultural productivity. Nowadays, about 50% of the total irrigated areas in Tunisia are considered at high risk for salinization. The aim of this thesis was to study soil management and salinity re

Idag finns ett stort antal företag som tillhandahåller digitala databaser med söktjänster där användare enkelt kan söka på personuppgifter som namn, adress eller personnummer och få fram omfattande information om svenska invånare. Uppgifter om civilstånd, bolagsinnehav, fastigheter samt om personen ifråga förekommer i tvistemål eller brottmålsdomar ingår bland sökresultaten. Företagen stödjer sig Today, many Swedish companies provide databases which function as search registers where users easily can search for personal data such as name, ad-dress or social security number and obtain comprehensive information on Swedish residents. Information on marital status, company ownership, real estates as well as whether the person in question is involved in civil or criminal cases are included in t

As robots increasingly integrate into our social environments, from factories to social spaces, there is a growing need to find ways to effectively collaborate in these dynamic environments. However, current robotics research is mostly aimed towards task and environment specific programming. Even the state-of-the-art collaborative robotics technologies lack or have a very rudimentary understanding