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A novel method of detecting alpha-1 antitrypsin deficiency of Z mutant (GAG(342)AAG) in a single PCR reaction using base-quenched probe

Background: Alpha-1 antitrypsin (A1AT) is a protease inhibitor that protects the tissues from degradation by neutrophil elastase under certain pathological process. Alpha-1 antitrypsin deficiency (A1ATD) could associate with both lung and liver pathogenicities. Of all the deficiency alleles, Z mutant is the most common variant and causes severe complications. Here, we described a novel and quick m

Re-dating the Pilgrimstad Interstadial with OSL: a warmer climate and a smaller ice sheet during the Swedish Middle Weichselian (MIS 3)?

Pilgrimstad in central Sweden is an important locality for reconstructing environmental changes during the last glacial period (the Weichselian). Its central location has implications for the Scandinavian Ice Sheet as a whole. The site has been assigned an Early Weichselian age (marine isotope stage (MIS) 5 a/c; > 74 ka), based on pollen stratigraphic correlations with type sections in continental

Methyl orange (CI acid orange 52) as a new organic semiconductor: Conduction mechanism and dielectrical relaxation

The structural, electrical and dielectrical properties of the methyl orange (MO) have been investigated by X-ray diffraction and thermal analysis methods. The electrical conduction and dielectrical relaxation mechanisms of the organic compound at various frequencies and temperatures were analyzed by impedance spectroscopy. The direct current (DC) electrical conductivity confirmed that methyl orang

Defining an International Standard Set of Outcome Measures for Patients with Hip or Knee Osteoarthritis: Consensus of the International Consortium for Health Outcomes Measurement Hip and Knee Osteoarthritis Working Group.

OBJECTIVE: To define a minimum Standard Set of outcome measures and case-mix factors for monitoring, comparing, and improving healthcare for patients with clinically diagnosed hip or knee osteoarthritis (OA) with a focus on defining the outcomes that matter most to patients. METHODS: An international Working Group (WG) of patients, arthroplasty register experts, orthopedic surgeons, primary care

Using the resistivity method for leakage detection in a blind test at the Rossvatn embankment dam test facility in Norway

Internal erosion is a cause of embankment dam failure, thus it is important to develop methods for seepage monitoring and internal erosion detection. In order to evaluate the potential of resistivity monitoring to give an early warning of such leakage/erosion, a test was undertaken on a rockfill embankment dam with a central glacial till core at the Rossvatn test facility in Norway. Three defects,

Evaluation of a School-Based Program Aimed at Preventing Depressive Symptoms in Adolescents

The aim of this pilot study was to evaluate the implementation of a universal school-based cognitive behavioral program whose target is to prevent depressive symptoms in adolescents. The study had a quasi-experimental design with pretest, posttest, and a 1-year follow-up and provides an illustrative calculation for the implementation costs of the intervention. Sixty-two students (aged 14) and seve

Unilocular adnexal cysts with papillary projections but no other solid components: is there a diagnostic method that can reliably classify them as benign or malignant before surgery?

Aim: To develop a logistic regression model for discrimination between benign and malignant unilocular solid cysts with papillary projections but no other solid components, and to compare its diagnostic performance with that of subjective evaluation of ultrasound findings (subjective assessment), CA 125 and the risk of malignancy index (RMI). Methods: Among the 3511 adnexal masses in the Interna

Efficient aquatic bacterial metabolism of dissolved low-molecular-weight compounds from terrestrial sources

Carboxylic acids (CAs), amino acids (AAs) and carbohydrates (CHs) in dissolved free forms can be readily assimilated by aquatic bacteria and metabolized at high growth efficiencies. Previous studies have shown that these low-molecular-weight (LMW) substrates are released by phytoplankton but also that unidentified LMW compounds of terrestrial origin is a subsidy for bacterial metabolism in unprodu

Personality traits and general intelligence as predictors of academic performance: A structural equation modelling approach

The aim of the present study was to investigate the extent to which personality traits, after controlling for general intelligence, predict academic performance in different school subjects. Upper secondary school students in Sweden (N=315) completed the Wonderlic IQ test (Wonderlic, 1992) and the IPIP-NEO-PI test (Goldberg, 1999). A series of hierarchical structural equation models showed that ge

Assessment of numerical schemes for solving the advection–diffusion equation on unstructured grids: case study of the Guaíba River, Brazil

In this work, a first-order upwind and a high-order flux-limiter schemes for solving the advection–diffusion equation on unstructured grids were evaluated. The numerical schemes were implemented as a module of an unstructured two-dimensional depth-averaged circulation model for shallow lakes (IPH-UnTRIM2D), and they were applied to the Guaíba River in Brazil. Their performances were evaluated by c

Valences of dopants in Eu2+ persistent luminescence materials

The existence and effect of different rare earth (R2+/3+/IV) ions in SrAl2O4:Eu2+, R3+ and M2MgSi2O7:Eu2+, R3+ (M: Sr, Ba) persistent luminescence materials was studied with XANES (x-ray absorption near edge structure) measurements at HASYLAB/DESY (Hamburg, Germany) and MAX-lab (Lund, Sweden). The experiments were carried out at 298K for selected rare earth (co-) dopants (Eu2+; Ce3+, Nd3+, Sm3+, D

A novel enterovirus and parechovirus multiplex one-step real-time PCR-validation and clinical experience

As the number of new enteroviruses and human parechoviruses seems ever growing, the necessity for updated diagnostics is relevant. We have updated an enterovirus assay and combined it with a previously published assay for human parechovirus resulting in a multiplex one-step RT-PCR assay. The multiplex assay was validated by analysing the sensitivity and specificity of the assay compared to the res

Prediction of hip displacement in children with cerebral palsy : development of the CPUP hip score

Hip displacement, defined in this study as a migration percentage (MP) of more than 40%, is a common, debilitating complication of cerebral palsy (CP). In this prospective study we analysed the risk of developing hip displacement within five years of the first pelvic radiograph. All children with CP in southern and western Sweden are invited to register in the hip surveillance programme CPUP. Incl

Best practices interventions to improve quality of care of people with dementia living at home.

Objective: To identify effective interventions which improve quality of care for people with dementia (PwD) living at home. METHODS: MEDLINE-(via PubMed), CINAHL, PsycINFO and ISI Web of Science databases were searched. Inclusion criteria: (1) randomized controlled trials; (2) published in English-language, peer-reviewed journals between 1990 and 2012; (3) evaluated strategies to improve quality

Mercury degassing rate from mineralized areas in the Mediterranean basin

Knowledge of the natural emissions of mercury in the Mediterranean basin, which has large cinnabar anomalies, has particular weight as these deposits could be an important source of atmospheric mercury. Data on the degassing rate of mercury from soils sampled both in mineralized areas (Mt. Amiata, Italy and Almaden, Spain) and rural areas are reported. Measurements were carried out 'in situ' using

Better Walking Performance in Older Children With Cerebral Palsy.

BACKGROUND: Children with cerebral palsy (CP) often walk with a slower speed and a higher energy cost. Their walking performance and choice of mobility method may vary in different environments. Independent mobility is important for activity and participation. QUESTIONS/PURPOSES: We described walking performance at different distances and environments in relation to gross motor function, CP subtyp

Reproducibility and accuracy of robot-assisted laparoscopic fertility sparing radical trachelectomy.

Objective. To assess the accuracy and reproducibility of robot-assisted laparoscopic abdominal fertility sparing radical trachelectomy in women with early stage cervical cancer. Methods. Relevant prospective clinical data from 13 consecutive women planned for robotic radical trachelectomy between 2007 and 2012 were compared with retrospective data from 12 consecutive women planned for vaginal radi

Passive houses in Sweden - Experiences from design and construction

The sector of residential buildings and service organizations uses 36% of the total energy in Sweden. In June 2006, it was decided by the Swedish parliament that the energy use in residential buildings and premises should decrease by 20% per heated unit area before 2020. To reach this goal, more energy efficient buildings must be produced as well as energy efficient improvements must be performed