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Polymerase chain reaction is superior to serology for the diagnosis of acute Mycoplasma pneumoniae infection and reveals a high rate of persistent infection

Background: Diagnosis of Mycoplasma pneumoniae (MP) infection is traditionally based on serology, which may require more than two weeks for diagnostic antibodies to develop. PCR-based methods offer earlier diagnosis. During a community outbreak of MP infection, we compared seminested and real-time PCR of oropharyngeal swabs with serology for diagnosis of MP infection at different time points after

Telomeres in Chironomus - studies on structure and evolution

Popular Abstract in Swedish Telomerer är en specialiserad DNA-protein struktur som finns i slutet av den linjära kromosomen. Trots att den telomera regionen inte innehåller några gener anses den vara oumbärlig för kromosomernas överlevnad. Telomererna skyddar kromosomerna från nedbrytning orsakad av enzymatiska attacker. Kromosomer utan telomerer har dessutom visats vara ytterst reaktiva och fuserThe telomeres, the physical ends of the chromosomes, consist in various species of short simple tandem repeats which are extended by telomerase. The Drosophila melanogaster telomeres, which instead are elongated by retroposons, are an exception. In Chironomus pallidivittatus and Chironomus tentans the telomeres contain 340/350-bp tandem repeats in blocks up to 200 kb in size. The structure of the

Severe Hemodilution - Clinical and Experimental Studies

Popular Abstract in Swedish Under det senaste decenniet har risker med och alternativ till allogent blod (blod från främmande givare) debatterats mycket pga. risken för smitta, allergiska reaktioner, samt starka indicier som talar för att blod från främmande givare kan påverka immunförsvaret hos mottagaren. Därför vill man gärna begränsa mängden tillfört givarblod under kirurgiska ingrepp på barn,In children, it is often desirable to minimize allogenic blood transfusion, and this thesis explores the physiology of an alternative method of managing perioperative blood loss: hemodilution with Ringer´s dextran. Methods Clinical studies: Arterial pressure, superior caval venous oxygen saturation (ScvO2) and blood lactate concentration (L) were studied during bone marrow harvesting (BMH) on 23

Estimation of leaf area index in southern Sweden with optimal modelling and Landsat 7 ETM+Scene

Populärvetenskaplig sammanfattning: Satelliter används ofta för att studera markanvändning och vegationsutbredning. För att öka satelliternas möjlighet att kvantifiera vissa parameterar hos vegetationen har en strålningsmodell utvecklats. Dessa modeller simulerar den reflekterade strålningen som satelliten registrerar. På grund av att reflektansprocessen är så kompliserad kräver modellen en stor mCanopy reflectance models are used as a means of estimating vegetation parameters and simulating reflectance signatures of vegetation surfaces. A forest canopy reflectance model is tested on deciduous forest collections in southern Sweden. A sensitivity analysis is performed to study how large influence each parameter has on the simulated reflectance. Thorough field measurements of the forest coll

Manlig lusta eller tabu? En undersökande uppföljning av sexköpslagen. Lag (1998:408).

January 1, 1999, a new sex purchase law was introduced in Sweden, Lag (1998: 408). The law implies that in a sex trade a crime is committed only by the consumer in the sex trade. The majority of consumers in prostitution are men and the suppliers are women. When prostitution is problematized it's usually from the woman's point of view and the perspective of the man is often overlooked. The

Verktyg för hantering av processförändringar

This report describes how you can create an application that uses Microsoft’s technologies, Windows Workflow Foundation, Windows Forms and Windows Presentation Foundation, to create and edit workflows. Companies have all sorts of processes, which support their business. Like create an E-mailaccount in the company’s network. When companies reorganize or streamline their business the processes needs

Coffee and tea consumption and the risk of ovarian cancer: a prospective cohort study and updated meta-analysis

Background: In 2007 the World Cancer Research Fund Report concluded that there was limited and inconsistent evidence for an effect of coffee and tea consumption on the risk of epithelial ovarian cancer (EOC). Objective: In the European Prospective Investigation into Cancer and Nutrition (EPIC), we aimed to investigate whether coffee intakes, tea intakes, or both are associated with the risk of EOC

Profile of TP53 gene mutations in sinonasal cancer

Genetic alterations underlying the development of the cancer of the nose and paranasal sinuses (sinonasal cancer, SNC), a rare cancer that can be included in the group of head and neck cancers, are still largely unknown. We recently reported that TP53 mutations are a common feature of SNC, with an overall frequency of 77%, and they show association to adenocarcinoma and wood-dust exposure [15]. In

Assessment of the colon innervation with serial biopsies above the aganglionic zone before the pull-through procedure in Hirschsprung's disease

Different types of colonic dysganglionosis, and in particular intestinal neuronal dysplasia (IND) have been blamed for certain postoperative complications after surgery for Hirschsprung's disease (HD). We prospectively assessed colon innervation above the aganglionic zone (AZ) before proceeding to pull-through (PT) in order to rule-out IND as a cause of those complications. We first used a two-sta

The usage of Stromgren photometry in studies of local group dwarf spheroidal galaxies - Application to Draco: a new catalogue of Draco members and a study of the metallicity distribution function and radial gradients

Aims. In this paper we demonstrate how Stromgren uvby photometry can be efficiently used to: 1. identify red giant branch stars that are members in a dwarf spheroidal galaxy; 2. derive age-independent metallicities for the same stars and quantify the associated errors. Methods. Stromgren uvby photometry in a 11 x 22 arcmin field centered on the Draco dwarf spheroidal galaxy was obtained using the

Outcome after HSCT in Philadelphia chromosome positive acute lymphoblastic leukemia in Sweden: a population-based study

Even in the tyrosine kinase inhibitor era, allogeneic hematopoietic stem cell transplantation (HSCT) is regarded as standard care for adult Philadelphia (Ph) positive acute lymphoblastic leukemia (ALL). In this retrospective national study, we have reviewed the outcome after HSCT in Sweden for adult Ph-positive ALL between 2000 and 2009. In total, 51 patients with median age 42 (range 20-66) years

Feasibility for mapping cartilage t1 relaxation times in the distal metacarpus3/metatarsus3 of thoroughbred racehorses using delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dgemric): normal cadaver study

Osteoarthritis of the metacarpo/metatarsophalangeal joints is one of the major causes of poor performance in horses. Delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) may be a useful technique for noninvasively quantifying articular cartilage damage in horses. The purpose of this study was to describe dGEMRIC characteristics of the distal metacarpus3/metatarsus3 (Mc3/Mt

An evaluation of the interference of hydroxycobalamin with chemistry and co-oximetry tests on nine commonly used instruments

The administration of hydroxocobalamin (OHCob), alone or with sodium thiosulfate, is a standard therapy for cyanide poisoning. OHCob is a red chromophore, and its interference with co-oximetric and colorimetric laboratory measurements has been evaluated in a few conflicting reports. The interference of OHCob was investigated in samples spiked with 10 different concentrations of OHCob (0-1500 mg/L)

Testing avian compass calibration: comparative experiments with diurnal and nocturnal passerine migrants in South Sweden.

Cue-conflict experiments were performed to study the compass calibration of one predominantly diurnal migrant, the dunnock (Prunella modularis), and two species of nocturnal passerine migrants, the sedge warbler (Acrocephalus schoenobaenus), and the European robin (Erithacus rubecula) during autumn migration in South Sweden. The birds' orientation was recorded in circular cages under natural clear

Colposcopy after cervical dysplasia: psychological aspects

The aim of this thesis was to conduct a prospective investigation of anxiety and its long-term effects on women referred for colposcopy after an abnormal cervical smear. The thesis comprises four articles, all based on a study of the current life situation, anxiety (Spielberger's state-trait anxiety inventory, STAI), depression (Montogomery Isberg Depression Rating Scale self-rate), psychosexualit

Effect of finite element model loading condition on fracture risk assessment in men and women: The AGES-Reykjavik study

Proximal femoral (hip) strength computed by subject-specific CT scan-based finite element (FE) models has been explored as an improved measure for identifying subjects at risk of hip fracture. However, to our knowledge, no published study has reported the effect of loading condition on the association between incident hip fracture and hip strength. In the present study, we performed a nested age-

Affinity binding of inclusion bodies on supermacroporous monolithic cryogels using labeling with specific antibodies

A new chromatographic method based on affinity supermacroporous monolithic cryogels is developed for binding and analyzing inclusion bodies during fermentation. The work demonstrated that it is possible to bind specific IgG and IgY antibodies to the 15 and 17 amino acids at the terminus ends of a 33 kDa target protein aggregated as inclusion bodies. The antibody treated inclusion bodies from lysed

Progress in methodology improved reporter gene assays used to identify ligands acting on orphan seven-transmembrane receptors.

Seven-transmembrane G-protein-coupled receptors play a central role in physiology by facilitating cell communication through recognition of a wide range of ligands. Even more important, they represent important drug targets. Unfortunately, for many of these receptors the endogenous ligands, and hence their functions, remain to be identified. These receptors are referred to as "orphan" receptors. A