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Determination of carotenoids in microalgae using supercritical fluid extraction and chromatography.

A method was developed based on supercritical fluid chromatography for quantitative determination of carotenoids in extracts of Scenedesmus sp. By utilizing the low backpressure in supercritical fluid chromatography, a C18 column and a 2-ethyl pyridine column were coupled in series. It was concluded that even minor changes in temperature had a substantial effect on selectivity. A standard mixture

The usage of Stromgren photometry in studies of local group dwarf spheroidal galaxies - Application to Draco: a new catalogue of Draco members and a study of the metallicity distribution function and radial gradients

Aims. In this paper we demonstrate how Stromgren uvby photometry can be efficiently used to: 1. identify red giant branch stars that are members in a dwarf spheroidal galaxy; 2. derive age-independent metallicities for the same stars and quantify the associated errors. Methods. Stromgren uvby photometry in a 11 x 22 arcmin field centered on the Draco dwarf spheroidal galaxy was obtained using the

Laminar burning velocity of gasoline and the gasoline surrogate components iso-octane, n-heptane and toluene

Laminar burning velocities have been measured using the heat flux method on a flat flame adiabatic burner. Measurements were done for iso-octane, n-heptane, toluene, a toluene reference fuel (i.e., a mixture of iso-octane, n-heptane and toluene) and a commercial gasoline. The laminar burning velocities of the toluene reference fuel were in correspondence with the laminar burning velocities of the

Fasciocutaneous Flap Reconstruction after Repair of Meningomyelocele: Technique and Outcome

Aims: The objectives of this study are to describe our technique in meningomyelocele (MMC) repair, analyzing the results and complications, as well as to study the effect of delay in operation on the complication rate. Patients and Methods: Between March 1997 and October 2012, 48 patients with MMC were treated at Jordan University Hospital by a combined neurosurgical and plastic surgical team. Pat

Affinity binding of inclusion bodies on supermacroporous monolithic cryogels using labeling with specific antibodies

A new chromatographic method based on affinity supermacroporous monolithic cryogels is developed for binding and analyzing inclusion bodies during fermentation. The work demonstrated that it is possible to bind specific IgG and IgY antibodies to the 15 and 17 amino acids at the terminus ends of a 33 kDa target protein aggregated as inclusion bodies. The antibody treated inclusion bodies from lysed

Progress in methodology improved reporter gene assays used to identify ligands acting on orphan seven-transmembrane receptors.

Seven-transmembrane G-protein-coupled receptors play a central role in physiology by facilitating cell communication through recognition of a wide range of ligands. Even more important, they represent important drug targets. Unfortunately, for many of these receptors the endogenous ligands, and hence their functions, remain to be identified. These receptors are referred to as "orphan" receptors. A

Does a long-term oscillation in nitrogen concentration reflect climate impact on submerged vegetation and vulnerability to state shifts in a shallow lake?

Various ecosystems, including shallow lakes, are suggested to possess alternative stable state dynamics. The response of such systems to environmental change is non-linear and not fully reversible, which calls for identification of feedback mechanisms and subtle changes connected to structural stability. Here, we used a 25-year data series on water chemistry to make inferences on processes prior t

The cost of feedback microwave thermotherapy compared with transurethral resection of the prostate for treating benign prostatic hyperplasia.

OBJECTIVE: To compare the efficacy of a new microwave thermotherapy for treating benign prostatic hyperplasia (BPH), the ProstaLund Feedback Treatment (PLFT, ProstaLund Operations AB, Lund, Sweden) and transurethral resection of the prostate (TURP) in a clinical trial to their effectiveness in clinical practice over 1 year, to estimate their cost over 1 year, and to evaluate the cost of re-interve

Statin use and risk of depression: a Swedish national cohort study

Background: Statin medications, used to prevent heart disease by reducing cholesterol, also reduce inflammation and protect against oxidative damage. As inflammation and oxidative stress occur in depression, there is interest in their potential to reduce depression risk. We investigated whether use of statin medications was associated with a change in the risk of developing depression in a very la

Retinal Function after Vitrectomy

PURPOSE:: To study retinal function after vitrectomy. METHODS:: Core vitrectomy was performed in 12 rabbits under standardized conditions using a vitreous cutting rate of either 600 or 1200 cuts/min. Full-field electroretinography (ERG) and multifocal electroretinography (mfERG) were performed pre- and postoperatively. Morphologic change was monitored by immunohistochemistry directed against glial

Two thymidine kinases and one multisubstrate deoxyribonucleoside kinase salvage DNA precursors in Arabidopsis thaliana.

Deoxyribonucleotides are the building blocks of DNA and can be synthesized via de novo and salvage pathways. Deoxyribonucleoside kinases (dNKs) salvage deoxyribonucleosides by transfer of a phosphate group to the 5' of a deoxyribonucleoside. This salvage pathway is well characterized in mammals but in contrast little is known about how plants salvage deoxyribonucleosides. We show that during salva

Tankar om utbildning och yrkesdrömmar hos tjejer födda 1995

The purpose of this study was to find out what girls born in 1995 thought about education and what they had for professional dreams. Could they see any problems to achieve their dreams? Did they think that being a woman could be a problem? Did their parents influence the choices that they want to make? Wei interview 9 girls at the age of 14 to 15 years old. We chose this group because they were on

The Strategic IS Decision-Making Process

Since the early 1980’s and up until today many organizations have realized the importance of paying more and more attention to the effective strategic planning of the information systems resources. There are several reasons for this that where recognized by Pyburn (1983), one was the high speed of technological progress both in regards to IT and Telecoms facilities. Another key factor, which is ve

Questioning the discriminatory accuracy of broad migrant categories in public health: self-rated health in Sweden

BACKGROUND: Differences between natives and migrants in average risk for poor self-rated health (SRH) are well documented, which has lent support to proposals for interventions targeting disadvantaged minority groups. However, such proposals are based on measures of association that neglect individual heterogeneity around group averages and thereby the discriminatory accuracy (DA) of the categori

Is palynology a credible climate proxy in the Subantarctic?

Pollen and spore analysis is the most successfully used palaeobotanical discipline for reconstructing Holocene vegetation and climate history throughout the world. Subantarctic islands are very specific areas. They are located in the circum-Antarctic Southern Ocean in latitudes that are under strong influence of the southern westerly winds, and are characterised by a treeless, phanerogam-poor flor

Benzochromenes from the roots of Bourreria pulchra

Two new benzochromenes, (6,6-dimethyl-2-methoxy-6H-benzo[c]chromen-9-yl)methanol (1), and 2-methoxy-6,6-dimethyl-6H-benzo[c]chromen-9-carbaldehyde (2), together with several already known metabolites, were isolated from the root extract of Bourreria pulchra (Boraginaceae). The structures of 1 and 2 were established on the basis of their spectroscopic data. Both were assayed for in vitro antiprotoz

First neuropathological description of a patient with Parkinson's disease and LRRK2 p.N1437H mutation.

The c.4309A>C mutation in the LRRK2 gene (LRRK2 p.N1437H) has recently been reported as the seventh pathogenic LRRK2 mutation causing monogenic Parkinson's disease (PD). So far, only two families worldwide have been identified with this mutation. By screening DNA from seven brains of PD patients, we found one individual with seemingly sporadic PD and LRRK2 p.N1437H mutation. Clinically, the patien