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The retrovirus forms its envelope by budding at the plasma membrane (PM). This process is primarily driven by its cytoplasmic core-precursor protein, Gag, as shown by the efficient formation of virus-like Gag particles in the absence of its envelope protein, Env. Most interestingly, several studies have demonstrated incorporation of various PM proteins into retrovirus, but the underlying mechanism

BACKGROUND: The influence of tobacco smoke has been investigated on the growth rate and histology of prostate cancer, both in untreated tumors and in those subjected to fractionated irradiation. METHODS: Twenty-five rats were implanted bilaterally with Dunning R3327 tumor fragments at 10 weeks of age. Approximately 3 months later, they were randomly allocated to two groups, one of which was expose

Lipid bilayers containing 5% nitrilotriacetic acid (NTA) lipids supported on SiO2 have been used as a template for immobilization of oligohistidine-tagged single-chained antibody fragments (scFvs) directed against cholera toxin. It was demonstrated that histidine-tagged scFvs could be equally efficiently coupled to an NTA-Ni2+-containing lipid bilayer from a purified sample as from an expression s

Stimulation of human fibroblasts by platelet-derived growth factor (PDGF)-BB leads to a down-regulation of PDGF beta-receptors and a concomitant appearance of intracellular granular accumulations of receptors, as determined by stainings with the mAb PDGFR-B2. The granules contained both the ligand and PDGF beta-receptors, as revealed by double-immunofluorescence staining, and were formed in respon

Structural properties of the acidic proline rich protein PRP-1 of salivary origin in bulk solution and adsorbed onto a negatively charged surface have been studied by Monte Carlo simulations. A simple model system with focus on electrostatic interactions and short-ranged attractions among the uncharged amino acids has been used. In addition to PRP-1, some mutants were considered to assess the role

An extensive small angle neutron scattering study is presented using contrast variation on the growth of nonionic microemulsion droplets as the temperature and thus spontaneous curvature is varied away from the emulsification failure boundary, EFB, toward zero spontaneous curvature. Two ternary systems are compared. They only differ by the chain length of the oil ( decane and hexadecane, respectiv

The electrostatic properties of charged surfactant micelles are investigated through titrations of fatty acid probes solubilized in the micelles. The titration process is followed by means of calorimetric measurements and by determining the pH values as a function of added base. This approach yields a complete thermodynamic description of the titration process. In particular, we find that the proc

The aqueous gallium(III)-acetate system was studied in 0.6 M Na(Cl) at 25 degreesC using a multi-technique approach, including potentiometry, IR spectroscopy, EXAFS spectroscopy and molecular orbital calculations. The potentiometric data were satisfactorily explained by a model which includes one mononuclear and one binuclear complex. The corresponding equilibrium constants defined according to th

Gel swelling experiments have been used to study the binding of ionic surfactants to a series of nonionic alkylacrylamide hydrogels of increasing hydrophobicity. The binding of hexadecyl trimethylammonium (C-16-TA(+)) to uncharged gels is sensitive to both the hydrophobicity of the gel and the counterion to the surfactant. There is a minimum hydrophobicity threshold below which binding of the surf

This paper presents data from the Austronesian language Seediq, spoken in taiwan, which has both VOS order and final particles. It will be argued that the coccurrence of these two features is unexpected under a pre-antisymmetric analysis, whereas it is easily captured under an analysis couched in Kayne's Antisymmetry model, under the assumption that VOS order is derived by raising the predicate to

Agarose gels have long been known to produce exceptionally large enhancements of the water H-1 and H-2 magnetic relaxation rates. The molecular basis for this effect has not been clearly established, despite its potential importance for a wide range of applications of agarose gels, including their use as biological tissue models in magnetic resonance imaging. To resolve this issue, we have measure

BACKGROUND: Phaeochromocytoma is sometimes not diagnosed before surgery and may present as an adrenal incidentaloma. The aim of this study was to investigate differences in clinical presentation and perioperative outcome in patients with subclinical and symptomatic phaeochromocytoma, and in patients operated with and without preoperative α-blockade. METHODS: This was a retrospective observational

Ecodesign policy for energy-using products so far has tended to focus on the energy efficiency requirements, but there is increasing interest in durability requirements as well. This exploratory study analyses whether and when long lifetimes are preferable when considering the trade-offs between durability and other important parameters such as costs and environmental impacts, examining the case o

Objectives: To investigate whether changes in complete blood count (CBC) in islet autoantibody positive children with increased genetic risk for type 1 diabetes are associated with oral glucose tolerance tests (OGTT) and HbA1c over time. Methods: The Environmental Determinants of Diabetes in the Young (TEDDY) study follows children with increased risk for type 1 diabetes in the United States, Germ

Swedish filmmaker Jan Halldoff's fiction film Korridoren (The Corridor) premiered in 1968 to a mainly positive Swedish reception. Shot at Karolinska Hospital and criticizing the Swedish healthcare system, the film became much debated and can furthermore be seen as a turning point in Swedish film history regarding the portrayal of medical institutions and professionals. In this article, the film an

Classical dendritic cells (cDC) can be classified into two major subsets: Irf8-dependent cDC1 and Irf4-expressing cDC2. Although these subsets play distinct roles in intestinal immune homeostasis, their functions in T cell-driven colitis remain unknown. To assess the role of IRF4 expression in cDC2 in T cell-driven colitis, CD11c-Cre.Irf4fl/fl and Irf4fl/fl mice were backcrossed onto a Rag-1-/- ba