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Mannan-penicillin G acylase neoglycoproteins were prepared by the conjugation of Saccharomyces cerevisiae mannan with enzyme penicillin G acylase using the reductive amination method. Eight neoglycoproteins preparations were obtained after gel chromatography. The preparations contained from 42 to 67% (w/w) saccharides and their molar masses varied from 283 to over 1000 kDa. Significant biospecific
Flexible goniometers are useful for direct movement measurements. Crosstalk due to rotation between the endblocks is well known. However, even without any rotation, some crosstalk can occur. The objective of this study was to elucidate the effect of, and compensate for, the inherent crosstalk in biaxial goniometers, with specific relevance for applications with one dominating movement direction. S
Isothermal microcalorimetry is now established as a useful technique for the characterization of the microbial activity in soil. A brief summary of publications from this field and of instruments used in such work is presented. Several experimental parameters that can form important sources for systematic errors are discussed and it is suggested that further method work is made in this area. In mo
Several architectural patterns have been proposed for the purpose of modeling autonomous robots. They do, however, lack support on anticipatory behavior. Anticipatory systems typically consist of hierarchical levels of units with similar structures but with different semantics. Close mapping between units of a reference system and components of software models put high demands on component indepen
RATIONALE AND OBJECTIVES. The authors established a rat model of the twitching reaction to contrast media in striated muscle and investigated the influence of nonionic contrast medium on the twitching reaction regarding the nonionic dimer iodixanol compared with the monomer iohexol and the dimer iotrolan. The authors also studied the influences from the electrolyte additions of iodixanol. METHODS.
Ferrous sulphate gel dosimetry has the potential for measurement of absorbed dose distributions in proton therapy. The chemical properties of the gel are altered according to the radiation dose and these changes can be evaluated in three dimensions using MRI. The purpose of this work was to investigate the properties of a ferrous gel used with clinical proton beams. The gel was irradiated with bot
Animal flight poses intriguing questions about biological adaptation, from how flight could have evolved to the morphological and physiological designs that enable flight to occur. Aerodynamic theory provides ecologists with a useful tool for understanding the basic physics of flight, but analysing flapping flight aerodynamics in birds is difficult, with interesting physiological complications. Re
Objective: The purpose of this investigation was to estimate the vertical deviation of the nasal floor and to register dental disturbances (i.e., hypo- and hyperdontia) in patients with an isolated soft tissue cleft lip. Design: Thirty patients with isolated cleft lips were randomly selected from the files of the Department of Maxillo-Facial Surgery and Jaw Orthopedics. As a matched control group,
Liquid crystalline cubic phase nanoparticles (known as Cubosome), based on glycerol monooleate and stabilized by a nonionic block copolymer (Pluronic F-127), adsorb intact on hydrophilic silica in the presence of an electrolyte, except at high pH. To characterize the nature of the interaction, four techniques were applied in situ: fluorescence microscopy, quartz crystal microbalance with dissipati
The rates of positive tuberculin skin test (TST) reactions and BCG scarring after BCG vaccination vary between studies and populations. Tuberculin reactivity and BCG scarring may be related to better child survival in low-income countries. We therefore studied determinants for TST reaction and scarring in Guinea-Bissau. In a cohort of children born in suburban Bissau from March 2000 to July 2002,
Neuropeptide Y (NPY) gene transduction of the brain using viral vectors in epileptogenic regions can effectively suppress seizures in animals, and is being considered as a promising alternative treatment strategy for epilepsy. Therefore, it is fundamental to understand the detailed mechanisms governing the release and action of transgene NPY in neuronal circuitries. Using whole-cell recordings fro
The substrate specificity of an alkaline protease, produced by Nesterenkonia sp. AL20 grown on chicken feather as the nutrient source, was assessed using oxidized insulin B-chain and derivatized peptide substrates. The initial cleavage of the. insulin chain was determined to be at Tyr(16) -Leu(17) and Tyr(26) -Thr(27), followed by Gln(4)-His(5), Phe(25)-Tyr(26) and Leu(15)-Tyr(16) bonds. An additi
BACKGROUND: Organic acid anhydrides (OAAs) are highly allergenic compounds used in the chemical industry. The OAAs probably act as haptens but the proteins that form conjugates with OAAs in vivo are still unknown. Conjugates between the anhydrides and serum albumins (SAs) have routinely been used when testing for OAA-specific antibodies. However, the use of SA as the carrier-protein in these tests
Background: Cyclosporine (CsA) absorption varies early after transplantation and can be accurately assessed by the area under the absorption curve (AUC). The 2-hour post-dose (C2) level of CsA in whole blood is reported to be a useful surrogate marker of CsA AUC in kidney and liver transplant monitoring, but should be further explored in thoracic organ recipients. Methods: In a 12-month study we i
Improving the survival rate and quality of life of patients with acute and chronic hepatic dysfunction still represents a challenge. Cell-based therapeutic hepatocyte transplantation has great potential to serve as a temporary support and “bridge” prior to a donor organ becoming available, or even as therapy for replacing a compromised liver. In this review we describe potential applications of hu
Two bypassing agents are currently available to circumvent the need for factor FVIII in hemophilia A patients with inhibitors: the activated prothrombin complex FEIBA VH and recombinant activated factor VII (NovoSeven (R)). Both products are highly effective in controlling bleeding in the presence of inhibitory alloantibodies, yet their hemostatic efficacy can be unpredictable. As the results of t
I combined neutral microsatellite markers with the major histocompatibility complex (MHC) class IIB to study genetic differentiation and colonization history in Atlantic salmon, Salmo salar, in the Baltic Sea and in the north-eastern Atlantic. Baltic salmon populations have lower levels of microsatellite genetic variation, in terms of heterozygosity and allelic richness than Atlantic populations,
The two objectives of the research reported in this article were to investigate the relationship between organizational creativity and innovation and psychological well-being of employees, as well as the relationship between organizational factors and organizational creativity and innovation. Ninety-five employees working in a high-tech field of industry participated in the study. The relationship
