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Background Despite recent successes with biological agents as therapy for autoimmune inflammatory diseases such as rheumatoid arthritis ( RA), many patients fail to respond adequately to these treatments, making a continued search for new therapies extremely important. Recently, the prevailing hypothesis that reactive oxygen species (ROS) promote inflammation was challenged when polymorphisms in N

Selektiv distribution är en distributionsform som innebär att de produkter som säljs inom systemet endast säljs till auktoriserade grossister och återförsäljare. De får i sin tur endast sälja till andra auktoriserade aktörer inom systemet eller till slutkund. För att bli auktoriserad ställs vanligtvis höga krav. De gäller bland annat krav på utbildad personal, försäljningsställets utformning och l

Den pågående klimatförändringens effekter kan komma att bli förödande för människan och forskare menar att det är människan själv som till stor del är orsaken till detta miljöproblem. Forskarna menar att mänskliga utsläpp av växthusgaser från framförallt förbränning av fossila bränslen är orsaken till den globala uppvärmning vi kan se idag. Av denna anledning är det viktigt att insatser görs på alThe ongoing climate change may have devastating consequences all over the world. Most scientists are in agreement that the cause of this global environmental problem is anthropogenic releases of greenhouse gases, of which the main contributor is the combustion of fossil fuels. In order to limit these effects, it is imperative to take action immediately, globally as well as locally. Earlier resear

Torr jord ger ifrån sig mer koldioxid när det regnar När det regnar på torr jord bildas under en kort tid mycket mer koldioxid än vad jorden vanligtvis avger. Detta fenomen har varit känt sen 1950-talet och kallas enkelt för torkning och återfuktning. Även om vi vet att det sker så vet vi väldigt lite om varför det sker. Till en början trodde man att det hade att göra med en ökad aktivitet hos ma

Organ-on-a-chip devices are predominately made of the polymer polymethylsiloxane (PDMS), exhibiting several attractive properties e.g., transparency, gas permeability, and biocompatibility. However, the attachment of cells to this polymer has proven challenging, especially for delicate primary cells e.g., small intestinal organoid-derived epithelial cells. Hence, a need to functionalize and coat t

Probiotics should bring ‘balance’ to the intestinal microbiota by stimulating beneficial bacteria, whilst mitigating adverse ones. Balance can also be interpreted as high alpha-diversity. Contrary, Escherichia coli is often regarded as an adverse component of the resident intestinal microbiota. The aim of the present study was to implement a mouse model for in vivo screening of Lactobacillus-strai

Background: Timely and safe elective health care facilitates return to normal activities for patients and prevents emergency admissions. Surgery is a cornerstone of elective care and relies on complex pathways. This study aimed to take a whole-system approach to evaluating access to and quality of elective health care globally, using inguinal hernia as a tracer condition. Methods: This was a prosp

With the identification of novel antibiotics from nature being pivotal in the fight against human pathogenic bacteria, there is an urgent need for effective methodologies for expedited screening of crude extracts. Here we report the development and validation of a simple and dye-free antimicrobial assay in 96-well microplate format, for both determination of IC50 values and high-resolution inhibit

The maturation process that occurs in most viruses is evolutionarily driven as it resolves several conflicting virion assembly requirements. During herpesvirus assembly in a host cell nucleus, micron-long double-stranded herpes DNA is packaged into a nanometer-sized procapsid. This leads to strong confinement of the viral genome with resulting tens of atmospheres of intra-capsid DNA pressure. Yet,

It has long been thought that respiratory infections are the direct result of acquisition of pathogenic viruses or bacteria, followed by their overgrowth, dissemination, and in some instances tissue invasion. In the last decades, it has become apparent that in contrast to this classical view, the majority of microorganisms associated with respiratory infections and inflammation are actually common

Class II antigens of the major histocompatibility complex (MHC) consist of two glycosylated, membrane-integrated polypeptide chains1. These cell surface-expressed molecules are involved in several immunobiological events involving cell-cell interactions2,3, most of which seem to require that genetically identical class II antigens, or other molecules controlled by the same region of the MHC, are e

This study examined the role of P fimbriae in the establishment of bacteriuria. Patients (n = 17) were subjected to intravesical inoculation with an asymptomatic bacteriuria strain, Escherichia coli 83972, or its P-fimbriated (pap+/prs+) transformants. As shown by groupwise analysis, the pap+/prs+ transformants established bacteriuria more rapidly than E. coli 83972 (P = 0.021) and required a lowe

The scFv antibody towards the Burkholderia pseudomallei exotoxin was previously constructed by phage display and exhibited good specificity towards the exotoxin. We report here the optimization of the scFv expression in an E. coli expression system. Four different E. coli strains (ER2537, TG1, HB2151, and XL1-Blue) were examined for optimal expression of the scFv protein. Two types of carbon sourc