Localization of phosphatidylserine binding sites to structural domains of factor X-a
Binding of short chain phosphatidylserine (C6PS) enhances the proteolytic activity of factor X-a by 60-fold (Koppaka, V., Wang, J., Banerjee, M., and Lentz, B. R. (1996) Biochemistry 35, 7482-7491). In the present study, we locate three C6PS binding sites to different domains of factor X-a using a combination of activity, circular dichroism, fluorescence, and equilibrium dialysis measurements on p
