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Production of heterologous thermostable glycoside hydrolases and the presence of host-cell proteases in substrate limited fed-batch cultures of Escherichia coli BL21(DE3)

Metabolic stress is a phenomenon often discussed in conjunction with recombinant protein production in Escherichia coli. This investigation shows how heterologous protein production, and the presence of host cell proteases is related to: i) IPTG induction, ii) cell mass concentration at the time of induction and iii) the presence of metabolites (glutamic acid or those from TSB) during the post ind

¿Una vivienda social incluyente en Liberia? El caso de una ciudad intermedia en Costa Rica

This article presents an empirical study, which analyzes the characteristics of those social housing projects implemented in the medium-sized city of Liberia, in Costa Rica, and their relationship to the territorial distribution of inequality and urban poverty. The dynamic economic growth of the province of Guanacaste and in particular of its capital, the city of Liberia, is widely recognized in C

Molecular and Morphological Characteristics of the De-Obstructed Rat Urinary Bladder—An Update

Many patients with outlet obstruction secondary to prostatic enlargement have lower urinary tract symptoms (LUTSs) and an increased frequency of micturition. The standard treatment is transurethral resection of the prostate (TURP), which alleviates obstruction and symptoms. However, after TURP, 20–40 percent of patients continue to experience LUTSs. The aim of the present study in rats was to iden

Hand and Arm Function in Persons with De Novo Parkinson’s Disease

Objective: To evaluate hand and arm function and its association with motor symptoms as well as activities of daily living (ADL) in de novo Parkinson’s disease (PD).Background: Motor symptoms and disability in upper limb are common in PD and usually assessed using Unified PD Rating Scale (UPDRS). Activities, like reaching, grasping, manipulating objects, writing, dressing, and eating, may be more

De minimis exception when using applied art in marketing materials

1. In order to prove a copyright exception not contemplated in the Act but based on long-standing practice the defendant must show strong evidence that goes beyond a usual practice in the business.2. The use of a copyright-protected work even in a commercial or marketing context can be of such secondary importance that – notwithstanding that the rights holder has not permitted the use – it would n