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In the past decade, many initiatives were taken for the development of antibodies for proteome-wide studies, as well as characterisation and validation of clinically relevant disease biomarkers. Phage display offers many advantages compared to antibody generation by immunisation because it is an unlimited resource of affinity reagents without batch-to-batch variation and is also amendable for high
Interactions between danger and pathogen-associated molecular patterns (DAMP and PAMP) and pattern recognition receptors such as Toll-like receptors (TLRs) are critical for the regulation of the inflammatory process via activation of NFκB and cytokine secretion. In this report, we investigated the capacity of LPS-free S100A9 (DAMP) protein to activate human and mouse cells compared to lipoprotein-
The human pathogen Haemophilus influenzae causes mainly respiratory tract infections such as acute otitis media in children and exacerbations in patients with chronic obstructive pulmonary disease. We recently revealed the crystal structure of H. influenzeae protein E (PE), a multifunctional adhesin that is involved in direct interactions with lung epithelial cells and host proteins. Based upon th
Serine proteases of the S1 family have maintained a common structure over an evolutionary span of more than one billion years, and evolved a variety of substrate specificities and diverse biological roles, involving digestion and degradation, blood clotting, fibrinolysis and epithelial homeostasis. We here show that a wide range of C-terminal peptide sequences of serine proteases, particularly fro
Popular Abstract in Swedish Sjögrens syndrom är en autoimmun sjukdom, vilket betyder att immunsystemet felreagerar och angriper kroppens egen vävnad. Nittio procent av dem som får diagnosen är kvinnor, av vilka de flesta är mellan 40 och 60 år. Vid Sjögrens syndrom, som tillhör en av våra vanligaste reumatiska sjukdomar, är det framförallt tår- och spottkörtlar som angrips och blir inflammerade. DPrimary Sjögren’s syndrome (SS) is a chronic autoimmune disease of unknown etiology. The disease primarily involves lachrymal and salivary glands, leading to dryness of the eyes and mouth, but a wide spectrum of exocrine and non-exocrine disease manifestations may be seen. A characteristic property of primary SS is the production of autoantibodies directed against intracellular, often DNA/RNA-modi
Wear in forming tools and contact problems between sheet-metal and tool-material are common problems in manufacturing industries and sheet-metal-parts production. In this paper recent tool material experiences are presented. Quantitative determination of wear has been performed using micro hardness indentions made on the tool surface prior to pressings and thereafter measured. Wear tests are perfo
Antibody-based microarrays have emerged as an established proteomic technology allowing multiplexed and sensitive profiling of complex proteomes, in a high-throughput and miniaturized manner. Recently, numerous applicative efforts have been pursued generating disease-associated protein signatures that now could be further explored for improved disease diagnostics, prognostics and classification.
Human papillomavirus (HPV) type 16 is the major cause of cervical intraepithelial neoplasia and anogenital cancers. The antibody response to papillomaviruses is a key determinant of protective immunity and forms the basis for prophylactic vaccine development as well as for seroepidemiology. The present thesis has evaluated different methods for HPV antibody measurements, determinants of the HPV an
Background: B-cells are essential in immunity against malaria, but which sub-sets of B-cells specifically recognize Plasmodium falciparum and when they appear is still largely unknown. Results: Using the flow cytometry technique for detection of P. falciparum specific (Pf+) B-cells, this study for the first time measured the development of Pf+ B cell (CD19+) phenotypes in Ugandan babies from birth
We analyze the convergence rate of the Dirichlet-Neumann iteration for the fully discretized one dimensional unsteady transmission problem. Specifically, we consider the coupling of two linear heat equations on two identical non overlapping intervals. The Laplacian is discretized using finite differences on one interval and finite elements on the other and the implicit Euler method is used for the
Dendritic cells (DC) are a heterogeneous lineage of bone-marrow-derived leukocytes that serve as the link between innate and adaptive immunity. They are professional antigen-presenting cells that play an important protective and regulatory role in both health and disease. This thesis is based upon six original papers that deal with the function and transcription of human DCs, with focus on their r
Popular Abstract in Swedish Upptäckten av Helicobacter pylori (Hp) (1982) har revolutionerat synen på uppkomsten av såväl magsår, magkatarr som magsäckskancer. Idag vet vi att Hp är en mycket starkt bidragande orsak till samtliga dessa sjukdomar och är en av våra vanligaste sjukdomsframkallande bakterier. Epidemiologiska studier har visat att mellan 50 och 80% av jordens befolkning är smittad. I SHelicobacter pylori and Borrelia burgdorferi s.l. can cause chronic infections by evading the immune system. These two human pathogens express a number of immunogenic cell surface proteins. The aims of this study were to gain further knowledge about these antigens and to analyse the serum antibody response in infected individuals. Both invasive and non-invasive diagnostic methods are available for
Popular Abstract in Swedish För att skydda sig mot omgivande virus, bakterier och parasiter har kroppen utvecklat ett speciellt försvar. Detta försvar utgörs av ett komplext nätverk av celler och lösliga komponenter som interagerar med varandra. En del av kroppens försvar utgörs av fysiska barriärer så som huden och av celler som är specialiserade på att ta upp och döda angripande mikroorganismer.The interaction between murine bone marrow-derived dendritic cells (DC) and the Gram negative bacteria Salmonella typhimurium and Escherichia coli have been characterised. These studies showed that DC phagocytosed and processed S. typhimurium and E. coli expressing defined epitopes for peptide presentation on major histocompatibility complex class I (MHC-I) and class II (MHC-II) molecules. Process
This article explores how the NT accounts of the rending of the veil in the Temple at the time of Jesus have been interpreted: as a sign of wrath, joy and grief.
Popular Abstract in Swedish Visst har väl vi väl alla någon gång funderat på vårt ursprung. Hur såg vi ut innan vi blev människor? Såg mina förfäder ut som jag eller var de håriga över hela kroppen? Är vi kanske kusiner med schimpanserna eller är våra närmaste ap-släktingar små silkesapor? Visst är detta intressanta frågor, men har du någonsin funderat på hur våra förfäder såg ut för ännu längre sFishes (Superclass Pisces) constitute the most numerous and morphologically diverse vertebrate group, yet only a small fraction of all molecular evolutionary studies concern piscine evolution. This thesis aims to investigate the vertebrate tree, through the analysis of mitochondrial (mt) DNA sequences. To this end the coding regions of the mt genome of a jawless fish, the hagfish (Myxine glutinosa
This research investigated how the employees of a company experienced a VR-model of an office building that was to be their future work place and how that VR-experience com¬pared with the employees’ experience of the completed building. About two years after the VR-showings the building was completed and the staff had moved into the building. The results for the employees who saw the VR-presentati
Cytokines play a major role in lymphoid development. Defects of the common gamma chain (gamma (c)) or of the JAK3 protein in humans have been shown to result in a severe combined immune deficiency (SCID), with a profound defect in T and natural killer (NK)-cell development, whereas B-cell generation is apparently unaffected (T-B+NK- SCID). While extensive molecular and biochemical analysis of thes
