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Reconstitution studies of a protein from domain fragments can furnish important insights into the distinctive role of particular domain interactions and how they affect biophysical properties important for function. Using isothermal titration calorimetry (ITC) and a number of spectroscopic and chromatographic tools, including CD, fluorescence and NMR spectroscopy, size-exclusion chromatography and
Disentangling the individual and interactive effects of multiple stressors on microbial communities is a key challenge to our understanding and management of ecosystems. Advances in molecular techniques allow studying microbial communities in situ and with high taxonomic resolution. However, the taxonomic level which provides the best trade-off between our ability to detect multiple-stressor effec
The ecology and biogeochemistry of lakes in the subarctic region are particularly sensitive to changes in the abundance and optical properties of dissolved organic matter (DOM). External input of colored DOM to these lakes is an extensively researched topic, but little is known about potential reciprocal feedbacks between the optical properties of DOM and internal microbial processes in the water.
development spectrum between 1990 and 2017 based on the Global Burden of Disease (GBD) 2017 estimates.METHODS: Injury mortality was estimated using the GBD mortality database, corrections for garbage coding and CODEm-the cause of death ensemble modelling tool. Morbidity estimation was based on surveys and inpatient and outpatient data sets for 30 cause-of-injury with 47 nature-of-injury categories
A desalting step using reversed phase chromatography is a common practice prior to mass spectrometry analysis of proteolytic digests in spite of the detrimental exclusion of the hydrophilic peptides. The detection of such peptides is also important for the complete coverage of protein sequences and the analysis of posttranslational modifications as inquired by regulatory agencies for the commercia
The majority of studies on mental health in deaf and hard-of-hearing (DHH) children report a higher level of mental health problems. Inconsistencies in reports of prevalence of mental health problems have been found to be related to a number of factors such as language skills, cognitive ability, heterogeneous samples as well as validity problems caused by using written measures designed for typica
Every cell is surrounded by a thin plasma membrane, protecting it from its surrounding. Specialized protein channels and transporters are embedded in this membrane, ensuring selective transport of molecules in and out of the cells. To maintain optimal hydration, water channels called aquaporins (AQPs) are present in every cell of our body. Thirteen isoforms of aquaporins exist in humans of which t
Seed dormancy is considered to be an adaptive strategy in seasonal and/or unpredictable environments because it prevents germination during climatically favorable periods that are too short for seedling establishment. Tropical dry forests are seasonal environments where seed dormancy may play an important role in plant resilience and resistance to changing precipitation patterns. We studied the ge
The field synergy principle has three criteria to qualitatively describe the essence of single-phase convective heat transfer enhancement. However, in practice these criteria are difficult to be applied to convective heat transfer analysis, because there are no corresponding indicators available to quantitatively describe them. Therefore, a unified formula for the field synergy principle was devel
Doctoral education in the technical fields has several distinct features, such as, the research is often projectbased, interdisciplinary, dependent on external funding, and affiliated with an industry. In other words, the epitome of a “modern doctorate” (Fillery-Travis, 2017; Lee, 2018). While literature on doctoral supervision in general is abundant (see e.g. Gatfield, 2005; Gurr, 2001; Ives &
Background: Obesity early in life has been linked to increased risk of developing multiple sclerosis (MS). Leptin and insulin are both associated with obesity, making them suitable candidates for investigating this connection. Objective: To determine if leptin and insulin are risk factors for relapsing–remitting multiple sclerosis (RRMS). Methods: In this nested case–control study using blood samp
Lubricin (PRG4) is a mucin type protein that plays an important role in maintaining normal joint function by providing lubrication and chondroprotection. Improper lubricin modification and degradation has been observed in idiopathic osteoarthritis (OA), while the detailed mechanism still remains unknown. We hypothesized that the protease cathepsin G (CG) may participate in degrading lubricin in sy
The pro-apoptotic Bax isoform Bax∆2 was originally discovered in cancer patients with a microsatellite guanine deletion (G8 to G7). This deletion leads to an early stop codon; however, when combined with the alternative splicing of exon 2, the reading frame is restored allowing production of a full-length protein (Bax∆2). Unlike the parental Baxα, Bax∆2 triggers apoptosis through a non-mitochondri
In order to gain insights into the biogeographic processes underlying biotic diversification in the Atlantic Forest (AF), we used a multi-locus approach to examine the evolutionary history of the White-shouldered Fire-eye (Pyriglena leucoptera) and the Fringe-backed Fire-eye (Pyriglena atra), two parapatric sister species endemic to the AF. We sequenced one mitochondrial, three Z chromosome-linked
Volatile compounds emitted by bacteria are often sensed by other organisms as odours, but their ecological roles are poorly understood1,2. Well-known examples are the soil-smelling terpenoids geosmin and 2-methylisoborneol (2-MIB)3,4, which humans and various animals sense at extremely low concentrations5,6. The conservation of geosmin biosynthesis genes among virtually all species of Streptomyces
Harmful algal blooms pose a threat to public health and the economy, affecting tourism, fisheries, and aquaculture. Complex environmental factors influence their occurrence, but current forecasting models often overlook the role of grazers. Chemical cues released by zooplankton, copepodamides, can trigger the production of toxins in harmful algae. Therefore, their presence in bivalves may enhance
This study aimed to examine the attitudes of social work students at Lund University towards autism and schizophrenia, with a focus on differences between the two diagnoses and how educational level and prior experience may explain variations in attitudes. The method the study used was a cross-sectional survey design and was conducted at the School of Social Work at Lund University, Sweden. Data w
This thesis develops a Fourier-feature DeepONet surrogate model for predicting two-dimensional steady laminar flow fields around a rotating triangular obstacle. The aim is to learn the mapping from the obstacle rotation angle to the corresponding pressure, x-velocity, and y-velocity fields. A CFD dataset containing 121 cases was generated using ANSYS Fluent, covering rotation angles from 0° to 120
Purpose: Altered auditory feedback research aims to identify methods to strengthen speakers’ awareness of their own voicing behaviors, diminish their perception of vocal fatigue, and improve their voice production. This study aims to compare the effects of two bone conduction devices that provide altered auditory feedback. Method: Twenty participants (19-33 years old, age: M [SD]=25.5 [3.85] years
