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The β-glucosidase TnBgl1A catalyses hydrolysis of O-linked terminal β-glycosidic bonds at the nonreducing end of glycosides/oligosaccharides. Enzymes with this specificity have potential in lignocellulose conversion (degrading cellobiose to glucose) and conversion of bioactive flavonoids (modification of glycosylation results in modulation of bioavailability). Previous work has shown TnBgl1A to hy
The recognition of carbohydrates by proteins is a fundamental aspect of communication within and between living cells. Understanding the molecular basis of carbohydrate-protein interactions is a prerequisite for the rational design of synthetic ligands. Here we report the high- to ultrahigh-resolution crystal structures of the carbohydrate recognition domain of galectin-3 (Gal3C) in the ligand-fre
Ribonucleotide reductases (RNRs) catalyze the reduction of ribonucleotides to deoxyribonucleotides, the building blocks for DNA synthesis, and are found in all but a few organisms. RNRs use radical chemistry to catalyze the reduction reaction. Despite RNR having evolved several mechanisms for generation of different kinds of essential radicals across a large evolutionary time frame, this initial r
Popular summary Estuarine eutrophication, the systemic limitation of oxygen in a body of water, has been increasingly monitored and scrutinized as an environmental concern needing acute and long term remediation efforts. The roots of eutrophication are largely attributed to anthropic sources within the watershed, sources that are products of modern civilization; such as agricultural and urban poll
This paper considers the effects of defects in the form of cracks on the failure properties in the thickness direction of multilayered board. The objective was to study how the peak stress value changes with various crack lengths under various load conditions. Specimens with manufactured cracks cut parallel to the machine-direction–cross-machine-direction plane were glued in the Arcan device and t
Glycerol-3-phosphate dehydrogenase is a biomedically important enzyme that plays a crucial role in lipid biosynthesis. It is activated by a ligand-gated conformational change that is necessary for the enzyme to reach a catalytically competent conformation capable of efficient transition-state stabilization. While the human form (hlGPDH) has been the subject of extensive structural and biochemical
C4b-binding protein (C4BP) is a plasma glycoprotein involved in regulation of the complement system. C4BP consists of seven alpha-chains and one unique beta-chain, all constructed of repeating complement control protein (CCP) modules. The beta-chain, made up of three CCPs, binds tightly to vitamin K-dependent protein S, a cofactor to anticoagulant activated protein C. When bound to C4BP, protein S
We present a solution for long-term direct current resistivity and time-domain induced polarization (DCIP) monitoring, which consists of a monitoring system and the associated software that automates the data collection and processing. This paper describes the acquisition system that is used for remote data collection and then introduces the routines that have been developed for pre-processing of
