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A concept based on the Peroxidase-chip (P-chip), antibody co-immobilization, competitive and enzyme-channeling principle was exploited to develop an integrated flow-through amperometric biosensor for detection of environmental pollutants such as s-triazine herbicides. In this concept, recombinant peroxidase is immobilized on the gold electrode (P-chip) in such a way that direct electron transfer i
In this work, the interfacing of a poly(ethylene glycol) (PEG)-phosphate aqueous two-phase system with hydrophobic interaction chromatography (HIC) for primary recovery of an intracellular protein was evaluated. As a model protein, a recombinant cutinase furnished with a tryptophan-proline (WP) peptide tag was used and produced intracellularly in Escherichia coli (E. coli). E. coli cell homogenate
In the weedy plant species Allium vineale (wild garlic), individuals may simultaneously produce sexually and asexually derived offspring, by seed and bulbils, respectively. In this study, genetic and genotypic diversity was determined in samples from 14 European A. vineale populations using nuclear (RAPD) and cytoplasmic (PCR-RFLP of cpDNA) markers to investigate the importance of the different re
To study different aspects of regional pre-synaptic brain 123I-β-CIT uptake on serotonin and dopamine re-uptake sites in drug-free suicide attempters in comparison with age- and sex matched control subjects, single photon emission computed tomography (SPECT) measurements were analysed for regional serotonin re-uptake (5HTT) and dopamine re-uptake (DAT) capacity (binding potential, BPlow asterisk)
Subarctic aerosol was sampled during July 2007 at the Abisko Research Station Stordalen field site operated by the Royal Swedish Academy of Sciences. Located in northern Sweden at 68 degrees latitude and 385 m above sea level (m asl), this site is classified as a semicontinuous permafrost mire. Number density, size distribution, cloud condensation nucleus properties, and chemical composition of th
Biotite, muscovite and vermiculite were treated in HCl and H2SO4 solutions at pH ranging between 7 and 1. The solid residue was studied using Mossbauer spectroscopy at room temperature. The spectra of the untreated samples, D0Bio, D0Mus and DOVer were fitted assuming quadrupole splitting distribution, QSD. Different fitting models with different combinations of Gaussian components for the Fe sites
Background and objectives: Fetal RHD genotyping of cell-free maternal plasma DNA from RhD negative pregnant women can be used to guide targeted antenatal and postnatal anti-D prophylaxis for the prevention of RhD immunization. To assure the quality of clinical testing, we conducted an external quality assessment workshop with the participation of 31 laboratories. Materials and methods: Aliquots of
Isothermal titration calorimetry (ITC) is an apt tool for a total thermodynamic description of self-assembly of atypical amphiphiles such as anionic boron cluster compounds (COSAN) in water. Global fitting of ITC enthalpograms reveals remarkable features that differentiate COSAN from classical amphiphiles: (i) strong enthalpy and weak entropy contribution to the free energy of aggregation, (ii) lo
Secretory leukocyte protease inhibitor (SLPI) is a well-known protease inhibitor. Its function is thought to be protease/protease-inhibitor balance. Free proteolytic activity, mainly pancreatic elastase, anionic trypsin and granulocytic elastase, has been demonstrated in faecal extracts from patients with ulcerative colitis. We wanted to verify that SLPI is actually secreted from normal human colo
The great chemist Glenn Seaborg has written a delightful little book "Man-made Transuranium Elements", published in 1963, in which he points out that: "The former basic criterion for the discovery of a new element - namely, chemical identification and separation from all previously-known elements - had to be changed in the case of lawrencium (element 103). This also may be true for elements beyond
The uncoupling proteins (UCPs) leak protons across the inner mitochondrial membrane, thus uncoupling the proton gradient from ATP synthesis. The main known physiological role for this is heat generation by UCP1 in brown adipose tissue. However, UCPs are also believed to be important for protection against reactive oxygen species, fine-tuning of metabolism and have been suggested to be involved in
Purpose: Previous studies using FLASH radiotherapy (RT) in mice showed a marked increase of the differential effect between normal tissue and tumors. To stimulate clinical transfer, we evaluated whether this effect could also occur in higher mammals. Experimental Design: Pig skin was used to investigate a potential difference in toxicity between irradiation delivered at an ultrahigh dose rate call
Immobilised 1,3-specific lipase from Rhizopus arrhizus was used as catalyst for the esterification of dl-glycero-3-phosphate and fatty acid or fatty acid vinyl ester in a solvent-free system. With lauric acid vinyl ester as acyl donor, a(w)<0.53 favored the synthesis of lysophosphatidic acid (1-acyl-rac-glycero-3-phosphate, LPA1) and the spontaneous acyl migration of the fatty acid on the molecule
Växtätare kan påverka ekosystemprocesser, dels genom långsiktiga förändringar av växtsamhällets sammansättning, men också på kortare sikt genom derasmatspjälkning av organiskt material som cyklas genom markens nedbryderorganismer och tillbaka till de primära producenterna. Särskilt störreväxtätare har studerats i stor utsträckning, men i vissa ekosystem som skogar kan de mindre ryggradslösa växtätHerbivory can influence ecosystem processes, partly through long-term changes of the plant community compositions, but also more rapidly through the herbivores’ digestive alteration of the organic matter that is cycled through the soil and back to the primary producers. In the Subarctic mountain birch (Betula pubescens ssp. czerepanovii) forest (SMBF) in Northern Fennoscandia, outbreaks by the geo
This article is dealing with the work of a Catholic St Joseph Sisters in the Nordic countries, particularly in Denmark and Sweden, from the sisters’ arrival in 1856 to the 1920:ies. These Catholic sisters belonged to a French congregation called La Congregation des Sœurs de Saint-Joseph de Chambéry, which was founded at the beginning of the nineteenth century and whose motherhouse was in Chambéry
Popular Abstract in Swedish Att fortsätta leva på mitt sätt. Medelålders kvinnor och menopaus/klimakteriet. Varje år passerar cirka 65 000 svenska kvinnor sin menopaus. Menopaus är en medicinsk term och avser tidpunkten för en kvinnas sista menstruation. Tidpunkten för menopaus kan därför i regel endast avgöras i efterhand, vanligtvis först efter ett års uppehåll av de naturliga menstruationernaTo deepen the understanding of menopause and its role in women's lives is the general aim of this thesis. Between 1995 and 2003, all women in two Swedish primary health care districts received a postal questionnaire, when they were 45, 50, 55 or 60. A total of 1681 women (76%) responded. Twenty-four women were given research interviews. The effects of menopause on quality of life (QoL) and number
The basis for the hypoxic relaxation of spontaneous activity in the rat portal vein was investigated by comparing responses to oxygen and the respiratory chain inhibitors amobarbital and cyanide. With the inhibitors, O2 consumption (FO2) is uniformly decreased throughout the cell mass, and thus O2 gradients in the tissue are avoided. Hence the effects are not to be attributed to all-or-none inhibi
Supported phospholipid bilayers (SPBs) have emerged as important model systems for studies of the natural cell membrane and its components, which are essential for the integrity and function of cells in all living organisms, and also constitute common targets for therapeutic drugs and in disease diagnosis. However, the preferential occurrence of spontaneous SPB formation on silicon-based substrate
