Search results

This thesis examines the effect of water quality, habitat and species interactions on the occurrence and abundance of stream fishes in southern Sweden. I found that improvements in water quality between the 1960s and 1990s have led to recolonization of streams by brown trout (Salmo trutta L.), stone loach (Barbatula barbatula L.) and eel (Anguilla anguilla L.). The response was greatest for brown

Popular Abstract in Swedish Biotisk och abiotisk stress hos mikroorganismer och växter.Various forms of stress, such as biotic and abiotic stress, were studied in microorganisms and plants to learn more about the underlying mechanisms of such stress and potential genetic engineering strategies towards stress tolerance. An antifreeze protein from winter flounder, expressed in E. coli, was subjected to directed evolution with NaCl as selective pressure. The resulting clones were teste

Popular Abstract in Swedish När en organism utsätts för stress, t. ex. vid hög temperatur, starkt solljus, torka eller köld, produceras särskilda hjälpar-proteiner, s. k. ¨stress-proteiner¨. Syntesen av cellens normala proteiner stängs av och istället produceras bara stress-proteiner. Dessa stress-proteiner är till för att skydda andra protein som skadas under stress. De s. k. ¨värme-stress¨ proteA specific group of heat shock proteins, the so called small heat shock proteins (sHsps), exists in all organisms but are especially abundant in plants. The sHsps prevent aggregation of other proteins (substrate proteins) during transient heat and oxidative stress. Molten globule forms of the substrate proteins are kept in a re-folding competent state by binding onto the outer surface of the sHsp.

Exaggerated acidosis due to preischemic hyperglycemia shortens the maturation time for cell death and turns selective neuronal damage into pannecrosis. Some issues concerning the mechanisms are yet to be clarified. The present study was designed to define critical thresholds of plasma glucose, to investigate the influence of preischemic hyperglycemia on ion transients, to evaluate the role of calc

Feeding, reproduction and accumulation of cyanobacterial toxins by the calanoid copepods Acartia bifilosa and Eurytemora affinis were studied during a cruise in the northern Baltic Sea. The experiments were carried out using both mixtures of natural plankton communities, mixtures containing the toxic Nodularia spumigena, and diets containing only the toxic cyanobacterium. Both copepod species had

We analyzed a comprehensive set of single-nucleotide polymorphisms (SNPs) and length polymorphisms in the interferon regulatory factor 5 (IRF5) gene for their association with the autoimmune disease systemic lupus erythematosus (SLE) in 485 Swedish patients and 563 controls. We found 16 SNPs and two length polymorphisms that display association with SLE (P < 0.0005, OR > 1.4). Using a Bayesian mod

Cystatin C, a major extracellular cysteine proteinase inhibitor, is deposited as amyloid in brain haemorrhage patients with hereditary cystatin C amyloid angiopathy (HCCAA). A disease-causing mutation on the genetic level results in the substitution Leu68-->Gln (L68Q) in cystatin C, which causes protein instability. Besides carrying the L68Q substitution, cystatin C in amyloid deposits isolated fr

Acute myeloid leukemia (AML) with inv(16)(p13q22) or the variant t(16;16)(p13;q22), is strongly associated with the FAB subtype M4Eo. A high incidence of CNS involvement was reported in the 1980s, but otherwise little is known about the pattern of extamedullary leukemia (EML) manifestations in this AML type. We have compiled clinical and cytogenetic data on 27 consecutive AML cases with inv(16)/t(

The Carex flava complex ( Cyperaceae) was used as a model system in a study of the effects of variation in species delimitation on genetic diversity estimates. The whole complex constitutes a syngameon within which gene flow may occur between taxa. Several more or less distinct evolutionary lineages in the complex are morphologically similar to each other and are often combined into broadly define

Sustained neurotrophic factor treatment in neurodegenerative disorders such as Parkinson's disease is likely to affect both degenerating and intact neurons. To investigate the effect of long-term glial cell line-derived neurotrophic factor (GDNF) overexpression on intact nigrostriatal dopamine neurons, we injected a recombinant lentiviral vector encoding GDNF, or green fluorescent protein, in the

Background Systemic lupus erythematosus (SLE) is a clinically heterogeneous disease in which the risk of disease is influenced by complex genetic and environmental contributions. Alleles of HLA-DRB1, IRF5, and STAT4 are established susceptibility genes; there is strong evidence for the existence of additional risk loci. Methods We genotyped more than 500,000 single-nucleotide polymorphisms (SNPs)

Testing models of macroevolution, and especially the sufficiency of microevolutionary processes, requires good collaboration between molecular biologists and paleontologists. We report such a test for events around the Late Cretaceous by describing the earliest penguin fossils, analyzing complete mitochondrial genomes from an albatross, a petrel, and a loon, and describe the gradual decline of pte

Helicobacter pylori attaches via lectins, carbohydrate binding proteins, to the carbohydrate residues of gastric mucins. Guinea-pigs are a suitable model for a H. pylori infection and thus the carbohydrate composition of normal and H. pylori infected gastric mucosa was investigated by lectin histochemistry. The stomach of all infected animals showed signs of an active chronic gastritis in their mu

Recent ChIP experiments of human and mouse embryonic stem cells have elucidated the architecture of the transcriptional regulatory circuitry responsible for cell determination, which involves the transcription factors OCT4, SOX2, and NANOG. In addition to regulating each other through feedback loops, these genes also regulate downstream target genes involved in the maintenance and differentiation