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The thermoresponsive copolymer of N-isopropylacrylamide (NIPAM) with 1-vinylimidazole (VI), poly(NIPAM-VI), synthesized by radical polymerization has been used to purify the histidine-tagged green flourescent protein (His-tag GFP) from recombinant E. coli by metal-chelate affinity precipitation. The purified protein was immobilized on the BIAcore sensor chip by carbodiimide coupling. Affinity bind
The Goal/Question/Metric paradigm is used for empirical studies on software projects. Support is given on how to define and execute a study. However, the support for running several subsequent studies is poor. V-GQM introduces a life-cycle perspective, creating a process, spanning several GQM studies. After the GQM study has been completed, an analysis step of the plan is initiated. The metrics ar
On the basis of the Agent-action-Objective (AaO) paradigm, it will be shown how the transformation of a word model to a geometric space description can be made the founda¬tion for an approach to informational invariants. In particular, the word model of a Causal Loop Diagram (CLD) has been used for simplifying the involved procedures. Since the explanation of a causal loop requires a verbal descri
In developing countries across the world people are depending on rivers as a source of water for drinking, cooking and bathing. This is also the case in the Indonesian part of the island Borneo where this study was made. The largest river is the river Kapuas which, together with its tributaries, supplies the area with water. The hydrology of these rivers is changing and the quality of the water in
The aim of this study is to examine how Swedish regions and cities are making use of Europe by various forms of lobbying efforts, and describe what role they are playing in EU’s multi-level system. The study initially concentrates on the development of the regional offices in general, and the Swedish offices in particular. Additionally, the study intends to discuss the possibilities and impli
In this article we elaborate on the process of institutionalization in the Higher Education performance evaluation ecosystem. We argue, we can observe a shift in how research evaluation functions, namely, from the contribution to knowledge to that of the societal influence of research. Such an axiological shift in evaluation goes to the very core of academic scholarship, as the commoditised neolib
How do we describe a location on earth? How do we describe a location on Earth? Geodetic reference system http://www.google.co.uk/imgres?imgurl=http://4.bp.blogspot.com/-v2sej--Hf9Y/TbGzN0FeN7I/AAAAAAAAAQ0/dXTA6XAP7Nk/s1600/earth-3d.gif&imgrefurl=http://ods-destruction-carbon-credits.blogspot.com/2011/04/happy-earth-day-from-remtec.html&usg=__1-2G0CU8X0-43x3j7NY4oN1vYS8=&h=1024&w=1024&sz=266&hl=en
https://www.nateko.lu.se/sites/nateko.lu.se/files/field2.pdf - 2026-07-07
The ambition to be recognized as a 'World City' or 'Global City' is rapidly increasing not the least among cities in the global south such as Hyderabad in India and Johannesburg in South Africa. While such a status seems promising for attracting foreign capital and for expanding the economic potential of urban areas, it may have adverse impacts on the hinterland and contribute to growing urban ine
The right to leave a country is enshrined in both international human rights law and its European counterpart. It is a right which is independent and does not require the individual exercising it to show that he or she is admissible in some other country. It is exercisable even in the absence of evidence of possible admission to a destination country. However, in Europe (and some other parts of th
Background: Research from sub-Saharan Africa has shown that persons with same-sex sexuality experience are at elevated risk for ill health due to sexual risk taking, stigma, and discrimination. However, studies of healthcare seeking among young people in this region with same-sex sexuality experience are limited.Objective: To identify determinants of unmet healthcare and sexual health counselling Background: Research from sub-Saharan Africa has shown that persons with same-sex sexuality experience are at elevated risk for ill health due to sexual risk taking, stigma, and discrimination. However, studies of healthcare seeking among young people in this region with same-sex sexuality experience are limited.Objective: To identify determinants of unmet healthcare and sexual health counselling
Scientific performance should not be measured by a one-dimensional metric such as publication, since it is a multi-dimensional phenomenon. A quantitative analysis of the activities of research groups in three scientific fields demonstrates in particular the importance of sufficient numbers of PhD graduates and of contributions to the infrastructure of the scientific community, in terms of editorsh
Discussion and debate regarding decision-making that is 'in the best interest of the child' and that also considers the child's voice in these decisions have increased in recent years. A study was undertaken to identify everyday situations in medical and nursing care in which children had been allowed to have a voice or been denied the right. Twenty-six hospitalized children, ages 6 to 17 years, a
