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Proteases are a group of enzymes that are involved in breaking down larger proteins into smaller polypeptides or single amino acids, and they are used in various applications. In this project, the potential of budding yeast Saccharomyces cerevisiae as host for the construction of protease libraries is evaluated. Three model proteases of bacterial origin are used to evaluate transformation efficien
The commissioning phase is the last phase of a construction project when all subsystems come together to be tested as a whole, working as a single entity. This phase is often the most crucial and time-sensitive part of the project as the construction occupies space in the factory and inflicts down¬time in processes without adding value. Faults found in this phase can be time-consuming to correct a
Analysis of peripheral blood smears is commonly used to aid physicians in diagnosing diseases, checking for infections and evaluating the function of organs. One object of interest in such analysis is the presence of red blood cell aggregates called rouleaux. CellaVision's product currently automates the traditionally resource-intensive process of blood smear analysis, which leads to improved
Lymph nodes (LNs) are believed to be the first organs targeted by colorectal cancer cells detached from a primary solid tumor because of their role in draining interstitial fluids. Better detection and assessment of these organs have the potential to help clinicians in stratification and designing optimal design of oncological treatments for each patient. Whilst highly valuable for the detection o
Transcription factor-based biosensors represent promising tools in the construction and evaluation of efficient cell factories for the sustainable production of fuels, chemicals and pharmaceuticals. They can notably be designed to follow the production of a target compound or to monitor key cellular properties, such as stress or starvation. In most cases, the biosensors are built with fluorescent
Febrile seizures represent the most common type of pathological brain activity in young children and are influenced by genetic, environmental and developmental factors. In a minority of cases, febrile seizures precede later development of epilepsy. We conducted a genome-wide association study of febrile seizures in 7635 cases and 83 966 controls identifying and replicating seven new loci, all with
Monoclonal gammopathy of undetermined significance (MGUS) is the precursor of multiple myeloma (MM) and related disorders. MGUS is characterized by asymptomatic paraproteinemia. In some cases, multiple paraproteins can be identified but the clinical implications of this phenomenon are poorly understood. In this study, we aim to inform the approach to this challenging MGUS subgroup by utilizing dat
In E. coli cells ribosomal small subunit biogenesis is regulated by RNA-protein interactions involving protein S7. S7 initiates the subunit assembly interacting with 16S rRNA. During shift-down of rRNA synthesis level, free S7 inhibits self-translation by interacting with 96 nucleotides long specific region of streptomycin (str) mRNA between cistrons S12 and S7 (intercistron). Many bacteria do not
Many cells express receptors for plasminogen (Pg), although the responsible molecules in most cases are poorly defined. In contrast, the group A streptococcal surface protein PAM contains a domain with two 13-amino acid residue long repeated sequences (a1 and a2) responsible for Pg binding. Here we identify the region in Pg that interacts with PAM. A radiolabeled proteolytic plasminogen fragment c
Publications Publications Selected publications Patching, G.R., Englund, M.P., & Hellström, Å. (2012). Time- and space-order effects in timed discrimination of brightness and size of paired visual stimuli. Journal of Experimental Psychology: Human Perception and Performance, 4, 915-940. Englund, M.P., & Patching, G.R. (2009). An inexpensive and accurate method of measuring the force of respons
https://www.psy.lu.se/en/sites/psy.lu.se.en/files/pi-publications_eng.pdf - 2026-07-22
Global gene expression profiles, consisting mainly of genes associated with proliferation, have been shown to subdivide histological grade 2 breast cancers into groups with different prognosis. We raised the question whether this subdivision could be done using a single proliferation marker, cyclin A. Furthermore, we combined cyclin A (CA), histological grade (G), and estrogen receptor-ER (E) into
The complex structural behavior of tau-[AgCu](similar to 0.92)V4O10 has been elucidated by single crystal X-ray diffraction and thermal analysis. The tau-phase region is apparently composed of several distinct phases and this study identifies at least three: tau(1rt) tau(2rt) and tau(1t), tau(1rt) and tau(2rt) have slightly different compositions and crystal habits. Both phases transform to Tit at
This paper presents a study of the turbulence field in an optical diesel engine operated under motored conditions using both large eddy simulation (LES) and Particle Image Velocimetry (PIV). The study was performed in a laboratory optical diesel engine based on a recent production engine from VOLVO Car. PIV is used to study the flow field in the cylinder, particularly inside the piston bowl that i
Metal chelate affinity precipitation (MCAP) has been successfully developed as a simple purification process for proteins that have affinity for metal ions. The present lack of widespread applications for this technique as compared to immobilized metal affinity chromatography (IMAC) may be related to the scarcity of well-characterized metal affinity macroligands (AML) and their applications to the
Enteral exposure of suckling rats to phytohaemagglutinin (PHA) has been shown to induce growth and precocious functional maturation of the gastrointestinal tract. The aim of the present study was to explore the mechanism of this action. Suckling rats, 14 d old, were fed a single dose of PHA (0.05 mg/g body weight) or saline. The binding of PHA to the gut epithelium and its effect on the morphology
In this paper, an amphiphilic peptide dendrimer with potential applications against multi-resistant bacteria such as Staphylococcus aureus was synthesized and studied on model cell membranes. The combination of quartz crystal microbalance and atomic force microscopy imaging during continuous flow allowed for in situ monitoring of the very initial interaction processes and membrane transformations
Compounds containing lone-pair elements such as TeIV are very interesting from the structural point of view, as the lone-pair nonbonding regions create low-dimensional geometrical arrangements. We have synthesized two new compounds with these features - Ba2Cu2Te 4O11Br2 (I) and Ba2Cu 2Te4O11-δ(OH)2δBr 2 (II, δ ≈ 0.57) - as members of the AE-M-Te-O-X (AE = alkaline-earth metal, M = transition metal
doi:10.1093/brain/awh649 Brain (2005), 128, 2977–2986 Factors affecting the clinical outcome after neural transplantation in Parkinson’s disease Paola Piccini,1 Nicola Pavese,1 Peter Hagell,3,4 Jan Reimer,3 Anders Björklund,5 Wolfgang H. Oertel,6 Niall P. Quinn,2 David J. Brooks1 and Olle Lindvall3 1MRC Clinical Sciences Centre and Division of Neuroscience, Faculty of Medicine, Imperial College,
https://www.wnc.lu.se/sites/wnc.lu.se/files/piccini_et_al_brain_2005.pdf - 2026-07-22
ATP and ADP inhibit, in varying degrees, several dehydrogenases of the central carbon metabolism of Lactococcus lactis ATCC 19435 in vitro, i.e. glyceraldehyde-3-phosphate dehydrogenase (GAPDH), lactate dehydrogenase (LDH) and alcohol dehydrogenase (ADH). Here we demonstrate mixed inhibition for GAPDH and competitive inhibition for LDH and ADH by adenine nucleotides in single inhibition studies. T
