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Activating anaplastic lymphoma kinase (ALK) receptor tyrosine kinase (RTK) mutations occur in pediatric neuroblastoma and are associated with poor prognosis. To study ALK-activating mutations in a genetically controllable system, we employed CRIPSR/Cas9, incorporating orthologs of the human oncogenic mutations ALKF1174L and ALKY1278S in the Drosophila Alk locus. AlkF1251L and AlkY1355S mutant Dros
PROBLEM: The embryo is protected from immunologic rejection by the mother, possibly accomplished by immunosuppressive molecules located in the placenta. We investigated the distribution and biochemical properties in placenta of the immunosuppressive plasma protein alpha 1-microglobulin.METHOD OF STUDY: Placental alpha 1-microglobulin was investigated by immunohistochemistry and, after extraction,
Pericytes conceivably play important roles in the tumour microenvironment of glioblastoma multiforme (GBM) by allowing for an aberrant vasculature and acting as a component in the perivascular niche that supports glioma stem-like cells. However, a lack of specific markers has hampered in-depth elucidation of the functional contribution of pericytes to GBM. This study provides a comprehensive compu
The endothelin receptor antagonist atrasentan improved kidney outcomes in the SONAR trial for type 2 diabetes and chronic kidney disease (NCT01858532), though individual responses varied. To identify molecular biomarkers of atrasentan response and outcome, we conducted a nested case-control proteomics study (N = 180) within the SONAR trial population and identified urinary clusterin (uCLU) as the
Endosomal escape and subsequent cytosolic delivery of small interfering RNA (siRNA) therapeutics is believed to be highly inefficient. Since it has not been possible to quantify cytosolic amounts of delivered siRNA at therapeutic doses, determining delivery bottlenecks and total efficiency has been difficult. Here, we present a confocal microscopy-based method to quantify cytosolic delivery of flu
The complex evolution of chloroplasts in microalgae has resulted in highly diverse pigment profiles. Freshwater raphidophytes, for example, display a very different pigment composition to marine raphidophytes. To investigate potential differences in the evolutionary origin of chloroplasts in these two groups of raphidophytes, the plastid genomes of the freshwater species Gonyostomum semen and Vacu
Infant Acute Lymphoblastic Leukemia (ALL) driven by the KMT2A::AFF1 onco-fusion is an aggressive, poor prognosis disease with few co-operative mutations. The fusion originates in utero, yet the embryonic initiating steps of disease development remain poorly understood. Here, we present a novel murine KMT2A::AFF1 model, that provides key insights into KMT2A::AFF1 pre-leukemia, relevant to human dis
Popular Abstract in Swedish För att celler ska kunna växa och dela sig behöver de många olika ämnen, till exempel vitaminer, mineraler och polyaminer. Polyaminer finns i alla celler och de krävs för att cellerna ska kunna dela eller specialisera sig. Cellerna kan både själva tillverka polyaminer och ta upp dem från omgivningen. I mänskliga celler finns framför allt de tre polyaminerna putrescin, sOur research group has previously shown that treatment with the polyamine biosynthesis inhibitors a-difluoromethylornithine (DFMO) or amidinoindan-1-one 2´-amidinohydrazone (CGP 48664) inhibited S phase progression before any other cell cycle phase was affected. This study was undertaken to further investigate the role of polyamines in the regulation of S phase progression and DNA synthesis. I hav
Precision-cut lung slices (PCLS) have gained increasing interest as a model to study lung biology/disease and screening novel therapeutics. In particular, PCLS derived from human tissue can better recapitulate some aspects of lung biology/disease as compared to animal models. Several experimental readouts have been established for use with PCLS, but obtaining high yield and quality RNA for downstr
External ATP has been proposed to be an autocrine regulator of glucose-stimulated insulin secretion and responsible for the synchronization of the Ca2+ rhythmicity in the P-cells required for a pulsatile release of insulin from the pancreas. The importance of external ATP for glucose-stimulated insulin release was evaluated in rats with the aid of 2-deoxy-N-methyladenosine3,5-bisphosphate (MRS 217
Uroteliala cancer, cancer i urinblåsan, är indelad i två större typer, icke-muskelinvasiv och muskel-invasiv cancer, med skilda biologiska och kliniska egenskaper. Prognos och behandling är i huvudsak baserade på patologiska kriterier. Det faktum att endast 20-25% av höggradiga tumörer med lågt tumörstadium bekräftas efter patologisk eftergranskning, och att 30% av patienterna med intermediärt tumUrothelial cancer, bladder cancer, is divided in two major subtypes, non-muscle invasive and muscle-invasive, with very distinct biological and clinical properties. Prognosis and treatment are mainly based on pathological criteria. The fact that only 20-25% of high grade low stage tumors are confirmed by review pathology, and that 30% of patients with intermediate stage tumors are upstaged after r
We have recently generated 5'lambda5-huTAC mice, which express the human CD25 (huTAC) gene under the control of the 5'-flanking region of the mouse lambda5-gene. The huTAC-transgene was expressed in pre-B cells but neither in mature B cells nor in T cells of these mice. In this report we demonstrate that the transgene is also transiently expressed by adult CD25+ CD3-CD4-CD8- (triple negative, TN)
Jens J. Dahlgaard Sweden Dr. Dahlgaard became a chair professor in financial auditing at Aarhus Uni- versity (1986-1991), and chair professor of a new established department of quality management in 1991 where he developed and was responsible for the first and only MSc program in quality management in Denmark from 1992 to 2000. He became later (2000) chair professor of the Division of Quality Tech
https://www.ses.lu.se/en/sites/ses.lu.se.en/files/qmod_2016_plenary_speakers.pdf - 2026-07-17
Haematopoietic stem cells are the source of all blood components including T-cells. When the T-cell programme is initiated in these cells, the expression of T-cell associated genes such as TCF7 is up-regulated, while genes opposing this fate are turned off. Subsequently, epigenetic marks on the gene BCL11B and its regulatory elements, disabling the expression of the gene, are removed and the trans
The current treatment options for ischemic stroke aim to achieve reperfusion but are time critical. Novel therapeutic approaches that can be given beyond the limited time window of 3–4.5 h are still an unmet need to be addressed to improve stroke outcomes. The lack of oxygen and glucose in the area of ischemic injury initiates a pathological cascade leading to blood-brain barrier (BBB) breakdown,
The electrode's composition and structure, affecting ion-conduction and water uptake and transport, is crucial for polymer electrolyte fuel cells. This study investigates the role of particles versus dispersed ionomer based on poly(arylene piperidinium) (PAP) for AEMFC. Mixed ionomer electrodes, consisting of linear PAP ionomers and crosslinked particles, are synthesized and evaluated in AEMF
In the present study the geographical distribution, abundance and composition of Gambierdiscus was described over a 600 km longitudinal scale in the Canary Islands. Samples for cell counts, isolation and identification of Gambierdiscus were obtained from five islands (El Hierro, Tenerife, Gran Canaria, Fuerteventura and Lanzarote). Average densities of Gambierdiscus spp. between 0 and 2200 cells g
Traditional network architecture design of Low Power Wide Area Networks (LPWAN) is incapable of dynamically scaling resources based on the served traffic and requires manual procedures for network capacity upgrades. Today's over-provisioning approach based on proprietary hardware (HW) would not be cost and energy efficient to cope with the ever-increasing scale of Internet of Things (IoT) devices
