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Proteases are a group of enzymes that are involved in breaking down larger proteins into smaller polypeptides or single amino acids, and they are used in various applications. In this project, the potential of budding yeast Saccharomyces cerevisiae as host for the construction of protease libraries is evaluated. Three model proteases of bacterial origin are used to evaluate transformation efficien
In March 2020 the Swedish public health authorities imposed the first of many restrictions on social interaction, as a way to limit the spread of the covid-19 pandemic. Coinciding with this is a new standard to work from home, as well as increased waves of migration out of Sweden’s major cities. This thesis aims to gain an understanding of current trends of urban emigration in relation to the Covi
Shrub range expansion, also known as shrubification, across arctic and subarctic ecosystems is expected to increase with climate change, with implications for vegetation structure, biodiversity, and soil carbon dynamics. Previous research has found a shrubification-induced decrease in organic matter stocks from tundra to forest in the organic horizon, prompting further investigation into whether t
Background:Engaging in social interaction has, for people with psychiatric disabilities, been shown to enhance well-being and the experience of meaning and to generally prevent the worsening of mental illness.Aim:The aim of the study was to investigate how day centre attendees differed from non-attendees regarding different aspects of social interaction and to investigate how occupational factors,
Measurements of differential production cross-sections of a Z boson in association with b-jets in pp collisions at root s = 7 TeV are reported. The data analysed correspond to an integrated luminosity of 4.6 fb(-1) recorded with the ATLAS detector at the Large Hadron Collider. Particle-level cross-sections are determined for events with a Z boson decaying into an electron or muon pair, and contain
A set of measurements for the production of a W-boson in association with high-transverse-momentum jets is presented using 140 fb-1 of proton–proton collision data at a centre-of-mass energy of s=13 TeV collected by the ATLAS detector at the LHC. The measurements are performed in final states in which the W-boson decays into an electron or muon plus a neutrino and is produced in association with j
The EU Mission on Climate-neutral and Smart Cities aims to enable 100 European cities to become climate-neutral by 2030. However, climate change has the potential to intersect with, exacerbate, and create new patterns of urban injustices, making climate justice a key concern in urban climate governance. Nevertheless, research on the integration of climate justice into urban mitigation strategies a
Sjögren’s syndrome (SS) is an exocrinopathy characterized by the hypofunction of salivary glands (SGs). Aquaporin-5 (AQP5); a water channel involved in saliva formation; is aberrantly dis-tributed in SS SG acini and contributes to glandular dysfunction. We aimed to investigate the role of ezrin in AQP5 mislocalization in SS SGs. The AQP5–ezrin interaction was assessed by immuno-precipitation and p
Lungultraljud har visat god precision och reproducerbarhet som diagnostiskt komplement till klinisk undersökning vid akut dyspné. Lungultraljud utförs bedside och är relativt lätt att lära sig, men kräver träning och strukturerat utförande för att inte resultera i felaktiga diagnoser.Lungultraljud baseras till största delen på tolkning av artefakter. Vid en normalt luftfylld lunga bildas horisonteUltrasound plays an important role in several medical fields. The heart was the first organ for which ultrasound gained clinical utility, followed by obstetric and gynecological applications. Shortly thereafter, abdominal organs and blood vessels became targets for ultrasound examination. The lung was long considered inaccessible for ultrasound due to its high air content. Work since the 1990s has
Diabetes mellitus is a result of various metabolic disorders causing continues hyperglycemia, which increases the risk for heart diseases, stroke, kidney failure and blindness. This disease is seen in a large section of world’s population and rapidly increasing worldwide. (1) The epigenetic marks can be transmitted by eukaryotic cell division (Mitosis) and also between generations of a species (
Background: Over half of children with rare genetic diseases remain undiagnosed despite maximal clinical evaluation and DNA-based genetic testing. As part of an Undiagnosed Diseases Program applying transcriptome (RNA) sequencing to identify the causes of these unsolved cases, we studied a child with severe infantile osteopetrosis leading to cranial nerve palsies, bone deformities, and bone marrow
Background: Researchers have shown an increased interest in involving professionals from outside academia in research projects. Professionals are often involved in research on ageing and health when the purpose is to address the gap between research and practice. However, there is a need to acquire more knowledge about what the involvement might lead to by exploring researchers’ experiences of inv
The first great ruler to encourage the adoption of Western culture and technology throughout Siam was King Narai, who also had a passion for astronomy. He showed this by encouraging French and other Jesuit missionaries, some with astronomical interests and training, to settle in Siam from the early 1660s. One of these was Father Antoine Thomas, and he was the first European known to have carried o
Objectives: The purpose of this study was to develop a procedure to investigate the occurrence, character and causes of magnetic resonance (MR) imaging incidents. Methods: A semi-structured questionnaire was developed containing details such as safety zones, examination complexity, staff MR knowledge, staff categories, and implementation of EU directive 2013/35. We focused on formally reported inc
The current study aimed to investigate the relationship between pediatric OCD and three emotion-related constructs; incompleteness, harm avoidance, and disgust sensitivity. Participants were 52 treatment seeking children and adolescents (aged 8-17 years) who were diagnosed with OCD or an anxiety disorder. Data was collected through self-report measures of incompleteness, harm avoidance, disgust se
This research seeks to investigate access to justice in Liberia, specifically delving into corruption in the Liberian Judiciary and its nexus to pretrial detention
The discovery of the Higgs boson h in 2012 further confirmed the remarkable accuracy of the Standard Model (SM). Despite this, some compelling excesses in multi-lepton final states were recorded in both major runs of the Large Hadron Collider (LHC). The excesses can be explained by the introduction of a model where a heavy boson X decays into a SM Higgs boson h and a singlet scalar S with Higgs-li
I denna kandidatuppsats undersöks den använda retoriken av den Nationella Kampanjen för rätten till laglig, säker och gratis abort i Argentina. Syftet med studien är att under- söka om den reproduktiva styrningen håller på att förändras. Det valda empiriska materialet består av ett dokument som skrivits av den Nationella Kampanjen för rätten till laglig, säker och gratis abort i Argentina. UppsatsThis bachelor thesis investigates the rhetoric used by the National Campaign for the right to legal, safe and free abortion in Argentina. The purpose of the study is to research if the reproductive governance of Argentina is in transition. The empirical material selected consist of a document written by the National Campaign for the right to legal, safe and free abortion in Argentina. The theoreti
