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Proteins G and PAB are bacterial albumin-binding proteins expressed at the surface of group C and G streptococci and Peptostreptococcus magnus, respectively. Repeated albumin-binding domains, known as GA modules, are found in both proteins. The third GA module of protein G from the group G streptococcal strain G148 (G148-GA3) and the second GA module of protein PAB from P.magnus strain ALB8 (ALB8-

Two-step steam pretreatment of softwood on laboratory scale has previously been shown to result in higher yields than one-step steam pretreatment. In this study, these results are verified on a larger scale. In an industrial process filtration and washing of the material between the two pretreatment steps are difficult without release of pressure. A worst case without filtration or washing was thu

The diagnosis of acute otitis media (AOM) is often difficult, depending heavily on the experience and skills of the examiner. However, it is important to identify episodes of AOM that involve the risk of complications and to treat these episodes appropriately. The present study was performed in order to evaluate the use of a rapid antigen assay for Streptococcus pneumoniae, the Binax NOW test, as

A scintillating fibre tracker is proposed to measure elastic proton scattering at very small angles in the ATLAS experiment at CERN. The tracker will be located in so-called Roman Pot units at a distance of 240 m on each side of the ATLAS interaction point. An initial validation of the design choices was achieved in a beam test at DESY in a relatively low energy electron beam and using slow off-th

We showed earlier that over-expression of protein kinase C (PKC) epsilon induces neurite outgrowth. The effect is mediated by a region (PKC epsilon PSC1V3) encompassing the pseudosubstrate, the two C1 domains and part of the V3 region, and is independent of the catalytic activity of the enzyme. In this region, residues immediately N-terminal of the C1b domain are crucial for neurite outgrowth. How

A general model for pressure drop calculations in siphons and riser tubes has been developed. The model takes into account different physical flow phenomena such as the inlet pressure drop and the pressure drop in the tube. For each phenomenon, we applied theory and established correlations to calculate the separate accelerational, frictional, gravitational, and centrifugal pressure drops. Model r

Background and Purpose-A low risk of recurrent stroke and death after lacunar infarction has previously been reported, but follow-up has been limited to less than or equal to5 years. Methods-One hundred eighty patients with pure motor stroke, collected between 1983 and 1986 from a hospital-based stroke registry, were followed up until at least 10 years after the index stroke. Two patients were los

Developing a reliable and quantitative assessment of the potential virulence of a malignancy has been a long-standing goal in clinical cytometry. DNA histogram analysis provides valuable information on the cycling activity of a tumor population through S-phase estimates; it also identifies nondiploid populations, a possible indicator of genetic instability and subsequent predisposition to metastas

Objective: To assess the clinical value of serial single photon-emission computed tomographic (SPECT) measurements after aneurysmal subarachnoid hemorrhage (SAH). Methods: Thirty-two patients were studied prospectively during the first 26 days after SAH with repeated SPELT measurements; clinical examinations, and transcranial Doppler recordings. Time trends were analyzed with a general linear mode

Background: The European Molecular Genetics Quality Network (EMQN) was formed in order to improve external quality assessment for molecular genetic testing in Europe. From 1999 to 2002 it received funding from the European Union under the Standards, Measurement and Testing programme (contract no. SMT4-CT98-7515). Since then, its maintenance has been supported through subscription of the participan

BACKGROUND: Yeast and bacteria elicit arachidonate release in macrophages, leading to the formation of leukotrienes and prostaglandins, important mediators of inflammation. Receptors recognising various microbes have been identified, but the signalling pathways are not entirely understood. Cytosolic phospholipase A2 is a major down-stream target and this enzyme is regulated by both phosphorylation

Stationary and mobile field gamma spectrometry is a useful tool for rapid estimation of environmental radioactivity inventories on and in the ground. A weak point however, is that the depth distribution of the activity in the ground must be known in order to calculate the true activity per unit area or unit mass from an observed photon fluence rate. A promising method for converting incoming spect

Precision agriculture requires detailed crop status information at high spatial and temporal resolutions. Remote sensing can provide such information, but single sensor observations are often incapable of meeting all data requirements. Spectral-temporal response surfaces (STRSs) provide continuous reflectance spectra at high temporal intervals. This is the first study to combine multispectral sate

Radio frequency identification (RFID) is a compelling technology for Internet of Things (IoT). Ultra-wideband (UWB) technology is one promising wireless technique for future RFID, especially for high-throughput sensing applications. On-off keying UWB RFID system provides high pulse rate but suffers severe collisions that limit the system throughput. This paper proposes to utilize low pulse rate co

The hydrogen abstraction of CH3OH by C-2 (A(3)Pi(u)) has been investigated by direct ab initio dynamics over a wide temperature range 200-3000 K. The potential energy surfaces (PESs) have been constructed at the UCCSD(T)/aug-cc-pVTZ//UMP2/6-311++G(d,p) levels of theory. Two different hydrogen abstractions on the methyl and hydroxyl sites of methanol are considered. For the methyl H-abstraction, it

Specific alterations (mutations, deletions, insertions) of virus genomes are crucial for the functional characterization of their regulatory elements and their expression products, as well as a prerequisite for the creation of attenuated viruses that could serve as vaccine candidates. Virus genome tailoring can be performed either by using traditionally cloned genomes as starting materials, follow

With the increasing output power of the integrated circuit chips, the heat flux involved is being accordingly increased. In such situation, the air has almost reached its limit of cooling capacity, and thus the liquid cooling technology incorporating microchannel heat sinks is desired to cool the electronic chips in order to remove more heat loads. However, these microchannel heat sinks are often