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A rapid and sensitive liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the qualitative and quantitative assay of cyproheptadine (CP) in pharmaceutical samples. Diphenylpyraline hydrochloride (DPP) was used as an internal standard (IS). Two multiple reaction-monitoring (MRM) transitions for each analyte were observed: 288.1/96.1 and 288.1/191.2 for
The human ribosomal protein S19 gene (RPS19) is mutated in approximately 20% of patients with Diamond-Black fan anemia (DBA), a congenital disease with a specific defect in erythropoiesis. The clinical expression of DBA is highly variable, and subclinical phenotypes may be revealed by elevated erythrocyte deaminase (eADA) activity only. In mice, complete loss of Rps19 results in early embryonic le
Dactylorhiza traunsteineri is an allotetraploid species that belongs to the large Dactylorhiza incarnata/maculata polyploid complex of the Eurasian genus Dactylorhiza (Orchidaceae). Here, eight polymorphic microsatellite loci were isolated using selective hybridization according to the FIASCO protocol (fast isolation by AFLP of sequences containing repeats) with slight modifications. The number of
It has been suggested that tryptophan 563 is sandwiched between residues R562 and A532 in Bruton's agammaglobulinemia tyrosine kinase (Btk). Mutations of the surrounding residues have been shown to cause X-linked agammaglobulinemia. Substitutions R562P and A582V were noticed to have impaired kinase activity. However, based on Western blot analysis, the mutant proteins were expressed at normal leve
Biogas production in anaerobic digestion in farm-scale units is typically performed under mesophilic conditions when used for producing domestic fuel and stabilizing animal waste for the use of digested manure as a fertilizer. Previous studies on the digestion of llama and cow manure have shown the feasibility of producing biogas under altiplano conditions (low pressure and low temperature) and of
This paper presents technical developments for the detection of formaldehyde (CH2O) using laser-induced fluorescence. The easily accessible third harmonic of the Nd:YAG laser at 355 nm was used for excitation of formaldehyde. In order to investigate potential background fluorescence, e.g., from large molecules such as polyaromatic hydrocarbons, special attention was paid to investigating the possi
The global carbon cycle is an important component of the Earth system and it interacts with the hydrology, energy and nutrient cycles as well as ecosystem dynamics. A better understanding of the global carbon cycle is required for improved projections of climate change including corresponding changes in water and food resources and for the verification of measures to reduce anthropogenic greenhous
Whole organ decellularization of complex organs, such as lungs, presents a unique opportunity for use of acellular scaffolds for ex vivo tissue engineering or for studying cell-extracellular matrix interactions ex vivo. A growing body of literature investigating decellularizing and recellularizing rodent lungs has provided important proof of concept models and rodent lungs are readily available fo
Background/Aim: To compare human papillomavirus (HPV) DNA detection in self-collected vaginal and urine samples with clinician-taken cervical samples in relation to histology. Materials and Methods: Self-collected vaginal, urine and clinician-taken cervical samples were analyzed from 218 women with the Cobas 4800 HPV test (Roche Molecular Diagnostics). Results: The sensitivity for detection of HPV
This paper presents two case studies of Swedish ICT 'gap exploiter' companies to provide a nuanced perspective in the investigation of ICT reuse business models and policies. Gap exploiters are third-party firms that create value through the re-utilization of existing products. While extending product life through the gap exploiter model is promising in the transition to a circular economy, busine
The likelihood that females will breed or how much they invest in reproduction can depend on the characters of their male partners. Such differential allocation may enhance or limit gene flow between hybridizing lineages, in particular when the lineages have diverged in sexually selected characters. Populations of the common wall lizard (Podarcis muralis) in some regions of Italy exhibit striking
The measurement of azimuthal correlations of charged particles is presented for Pb-Pb collisions at sNN=2.76 TeV and p-Pb collisions at sNN=5.02 TeV with the ALICE detector at the CERN Large Hadron Collider. These correlations are measured for the second, third and fourth order flow vector in the pseudorapidity region |η| < 0.8 as a function of centrality and transverse momentum pT using two obser
The ESS Linac project, phase I, requires 12 long pulse klystron modulators with compact footprint, high pulse power and improved quality both on the output pulse waveform and on the input AC power line. Conventional long pulse modulators are typically based on HV pulse transformers and commonly exhibit poor efficiency, low power density, large footprint and cost, with still limited performance on
A new method to determine the concentration of the free protein S in plasma is described. It is an enzyme-linked ligandsorbent assay (ELSA) which utilises the protein S binding capacity of the natural ligand C4b-binding protein (C4BP) to capture the free protein S from plasma samples. The use of C4BP as ligand in the assay is possible due to the high affinity (Kd = 0.1 nM) of the interaction betwe
In 2015 Afghans were the second largest group of asylum seekers in Sweden (and Europe). In this article, I analyze interviews conducted in early 2017 in Scania County with six adult male Afghan asylum seekers, an executive officer at the Swedish Migration Agency, the head of a private asylum seeker camp, and a voluntary worker. I show how the asylum seekers made their way to Sweden not so much thr
The paper presents results from a comparative study of selected biological and technical functions for thermal adaptability, which are based on an extensive literature review in biology and façade engineering. Several selected biological role models have been analysed in detail to identify their principles for dealing with thermal adaptation. These principles have been further abstracted through s
