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We have developed a novel allele-specific primer elongation protocol using a DNA polymerase on oligonucleotide chips. Oligonucleotide primers carrying polymorphic sites at their free 3'end were covalently bound to glass slides. The generation of single-stranded targets of genomic DNA containing single nuclotide polymorphisms (SNPs) to be typed was achieved by an asymmetric PCR reaction or exonucle
The degradation of hydroxyethyl cellulose (HEC) using sodium persulfate (NaPS) as free radical generator was studied at 60, 70 and 80 degrees C with different NaPS/HEC ratios. During the degradation reaction samples were withdrawn at regular intervals. The amount of persulfate remaining was analyzed by titration and the evolution of the HEC molecular weight distribution and viscosity was followed
The vascular effects of endothelins (ET) are in mammals mediated via two receptor subtypes, endothelin A (ETA, mainly constrictive) and endothelin B (ETB, mainly dilating) receptors. We have examined the presence of ETA and ETB receptor mRNA using the reverse transcription polymerase chain reaction (RT-PCR) in both normal human cerebral arteries and cerebral arteries from patients with cerebrovasc
An isochromosome of the long arm of chromosome 17, i(17q), is the most frequent genetic abnormality observed during the disease progression of Philadelphia chromosome-positive chronic myeloid leukemia (CML), and has been described as the sole anomaly in various other hematologic malignancies. The i(17q) hence plays a presumably important pathogenetic role both in leukemia development and progressi
In this study, we investigated the interaction of fengycin, a lipopeptide produced by Bacillus subtilis, with lipid monolayers using the Langmuir trough technique in combination with Brewster angle rnicroscopy. Thermodynamic analyses were performed to get further information about the mixing behavior and the molecular interactions between the two components. The effect of fengycin on the structura
The question that links all aspects of this thesis is how democratic nations implement international human rights law to protect their foreign migrant workers from unequal treatment. First, I examine international legal principles that are primarily focused on the principle of non-discrimination in international law. I describe and critically analyse previous publications, postulations, universal
Popular Abstract in Swedish Kärleksdofter och varningsljud i bekämpning av nattfjärilar Jag har studerat hur populationer av skadegörande nattfjärilar kan bekämpas med miljövänliga metoder där djurens egna sinnessystem manipuleras. Liksom många andra insekter kommunicerar nattfjärilar med artspecifika doftsignaler (feromoner) vid parning. Honan avger doften i enstaka nanogram (miljarddels gram!) The impact of olfactory and auditory cues on the mating behaviour in two species of moths, Agrotis segetum (Noctuidae) and Plodia interpunctella (Pyralidae), was investigated to evaluate their potential use in integrated pest management. The flight behaviour of male A. segetum under natural and pheromone-based mating disruption conditions was monitored by harmonic radar. Individual males were equi
Implantation of demineralized bone matrix in rodents elicits a series of cellular events leading to the formation of new bone inside and adjacent to the implant. This process is believed to be initiated by an inductive protein present in bone matrix. It has been suggested that local growth factors may further regulate the process once it has been initiated. This investigation was designed to study
Nässelqvist’s contribution examines different models for understanding the oral delivery of New Testament writings. He finds evidence in favor of the existence in antiquity of both oral performance from memory and public reading directly from a manuscript. These types of oral delivery not only involved different delivery practices, but were also used in dissimilar settings for distinct text genres
We examined undergraduate participants' (N = 98) self-related cognitive response to positive information about the self and how this response is related to global self-esteem and trait anxiety. Positive information about the self was defined as information that attributes positive qualities to the self or positive experiences for the self. The responses were measured by an open-ended questionnaire
Objectives: The effects of chronic cobalt exposure were studied in an isolated working model in a modified Langendorff preparation. Methods: Thirty-six rats were given either a conventional (control group) or a cobalt supplemented diet (cobalt group; 40 mg CoSO4 x 7 H2O/kg body weight/day). Sixteen of the animals were sacrificed and their hearts were excised after 16 weeks and the remaining 20 aft
The activity and adsorption of three variants of human carbonic anhydrase (HCA) with similar topology but variation in charge and stability were studied in the presence of carboxyl-modified polystyrene nanoparticles of different sizes ranging from 25 nm to 114 nm. The balance of forces driving the adsorption of carbonic anhydrase variants is affected by the physicochemical properties of the protei
Electrical machines, chokes and induction heaters are found in most homes,offices and factories all over the world. They are used to create movement, filtrate the power or to generate heat. A typical unit consist of a coil and a flux conductor material. Some of the materials have been established for over 100 years, while others are only a couple of decades old.A new flux conductor material has be
Long-term oxygen therapy (LTOT) ≥ 15 h/day improves survival in hypoxemic chronic obstructive pulmonary disease (COPD). LTOT 24 h/day is often recommended but may pose an unnecessary burden with no clear survival benefit compared with LTOT 15 h/day. The aim was to test the hypothesis that LTOT 24 h/day decreases all-cause, respiratory, and cardiovascular mortality compared to LTOT 15 h/day in hypo
Water evaporation concerns all land-living organisms, as ambient air is dryer than their corresponding equilibrium humidity. Contrarily to plants, mammals are covered with a skin that not only hinders evaporation but also maintains its rate at a nearly constant value, independently of air humidity. Here, we show that simple amphiphiles/water systems reproduce this behavior, which suggests a common
Hippocampal extracellular levels of noradrenaline (NA), 5-hydroxytryptamine (5-HT), and 5-hydroxyindoleacetic acid (5-HIAA) were monitored with the microdialysis technique in freely moving rats. In one experiment 30 min samples were collected during 24 h of continuous perfusion, and the monoamine output was compared to the behavioural activity state, as arbitrarily classified in three categories:
Calreticulin is a multifunctional protein mainly localized in the endoplasmic reticulum in eukaryotic organisms, except yeasts. The protein comprises three distinct regions: the N-terminal N domain, the C-terminal C domain, and the central P domain, reflecting the functional diversity of calreticulin. The protein has been implicated in over 40 intra- and extracellular processes in mammalian cells,
