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BACKGROUND: In animals, we have previously done successful lung transplantations using organs from non-heart-beating donors. We have also developed an ex-vivo system of assessing the function of such organs before transplantation. The next stage was to try the technique in human beings. Bearing in mind the sensitive ethical issues involved, our first aim was to find out what procedures would be ac
Objective: To characterise uterine proteoglycans and changes therein during pregnancy and labour. Study Design: Uterine samples were collected from 6 non-pregnant, 10 term-pregnant and from 10 women in active tabour. The proteoglycans were extracted by 4 M guanidine hydrochloride and precipitated with Alcian Blue. They were separated by electrophoresis and identified by Western blotting. Results:
The influence of solar radiation on the ability of dissolved organic matter (DOM) to support bacterial growth, was studied in the eastern Atlantic sector of the Southern Ocean during the SWEDARP 1997/1998 cruise with SA Agulhas December 1997-February 1998. Vertical profiles of water samples (2-3000m) were obtained from the Spring Ice Edge (SIE, 60degreesS, high chlorophyll-a) and from the Winter I
This study describes the development of a high throughput procedure for the extraction, purification, and quantification of all 16 polycyclic aromatic hydrocarbons (PAHs) on EPA's priority list, subject to biodegradation assays in a creosote-contaminated soil. We used various combinations of solvents, temperatures, ultrasonication, tumbling, saponification, vacuum centrifugation, adsorption chroma
C4b-binding protein (C4BP) is a regulator of the classical complement pathway C3 convertase (C4bC2a complex). It is a disulfide-linked polymer of seven alpha-chains and a unique beta-chain; the alpha- and beta-chains are composed of eight and three complement control protein (CCP) domains, respectively. To elucidate the importance of the polymeric nature of C4BP and the structural requirements for
beta-Glucosidase genes of fungal origins were isolated and heterologously expressed in Saccharomyces cerevisiae to enable growth on the disaccharide, cellobiose. To promote secretion of the beta-glucosidases, the genes were fused to the secretion signal of the Trichoderma reesei xyn2 gene and constitutively expressed from a multi-copy yeast expression vector under transcriptional control of the S.
Stamp fabrication for nanoimprinting can be significantly simplified, when specialized crosslinking polymers are applied to pattern definition. The polymer patterns can be used as stamps themselves. Two possibilities are reported: (1) An e-beam sensitive resist was developed, which enables the fabrication of polymer-on-silicon stamps. Patterns with a feature size of 70 nm could be created. (2) Ful
The effect of Phytophthora species, soil chemistry, precipitation and temperature on the vitality of oak was evaluated in 32 oak stands in southern Sweden. In addition, the relationship between the occurrence of Phytophthora species and soil conditions was determined. The results showed that there was a weak association between the presence of P. quercina, the most frequently recovered Phytophthor
Many small farmland ponds are built for nutrient retention, the conservation of biodiversity or both, yet they are relatively neglected habitats. For example, little is known about the potential for ponds to influence populations of beneficial terrestrial insects, deliver ecosystem services across the aquatic-terrestrial boundary and affect crop yield in insect-pollinated cash crops.We assessed wh
PURPOSE: The aim of this study was to evaluate how relatives of patients with an ostomy rated the various aspects of care, how they perceived the quality of the care provided, and how they ranked their involvement in the care. Patients' satisfaction was also sought, particularly their perception of relatives' involvement.DESIGN: Descriptive cross-sectional.SETTINGS AND SUBJECTS: The study comprise
BACKGROUND: High body mass index (BMI) and low physical fitness are risk factors for stroke, but their interactive effects are unknown. Elucidation of interactions between these modifiable risk factors can help inform preventive interventions in susceptible subgroups.METHODS: National cohort study of all 1,547,294 military conscripts in Sweden during 1969-1997 (97-98% of all 18-year-old males). St
BACKGROUND: Globally, the most common method for in situ fixation of slipped capital femoral epiphysis (SCFE) is a threaded screw, which causes physeal arrest. The standard treatment in Sweden is unthreaded fixation using the Hansson hook-pin, which leads to continued growth of the femoral neck. Our purpose was to study remodeling during the remaining growth after fixation with the Hansson hook-pi
BACKGROUND AND PURPOSE: Genome-wide association (GWA) studies have identified a few risk loci for ischaemic stroke, but these variants explain only a small part of the genetic contribution to the disease. Coding variants associated with amino acid substitutions or premature termination of protein synthesis could have a large effect on disease risk. We performed an exome array analysis for ischaemi
Biogenic Volatile Organic Compounds (BVOCs) are trace gases other than CO2 and CH4 produced and emitted by the biosphere, where the amounts released depend on climatic factors such as temperature and solar irradiation. However, interpretation of leaf-level measurements is currently hampered by factors such as large within-genotypic variability, measurement height and time in the season. A campaign
Objectives: Rituximab (RTX) is a biological treatment used off-label in patients with systemic lupus erythematosus (SLE). This survey aimed to investigate the off-label use of RTX in Europe and compare the characteristics of patients receiving RTX with those receiving conventional therapy. Methods: Data on patients with SLE receiving RTX were taken from the International Registry for Biologics in
We measured volatile organic compounds (VOCs), carbon dioxide (CO2) and carbon monoxide (CO) at an urban background site near the city centre of Helsinki, Finland, northern Europe. The VOC and CO2 measurements were obtained between January 2013 and September 2014 whereas for CO a shorter measurement campaign in April–May 2014 was conducted. Both anthropogenic and biogenic sources were identified f
Background: Carpenter ants (genus Camponotus) are considered to be omnivores. Nonetheless, the genome sequence of Blochmannia floridanus, the obligate intracellular endosymbiont of Camponotus floridanus, suggests a function in nutritional upgrading of host resources by the bacterium. Thus, thestrongly reduced genome of the endosymbiont retains genes for all subunits of a functional urease, as well
Popular Abstract in Swedish Ser man bakåt i historien har människan alltid försökt reparera eller ersätta vävnader i kroppen vid skador. En av de äldsta rapporterna om användande av biomaterial är runt 4000 år gammalt. I detta fall användes linnefibrer som suturmaterial. Från början användes naturliga material som collagen, silke, hår, bomull och olika cellulosamaterial. Under senare hälften av 19The aim of the this thesis was surface modification of a biomedical polyurethane (PUR), Pellethane 2363-80AE*, to improve the biocompatibility. Surfaces modified by poly(ethylene oxide) (PEO) were prepared either by grafting PEO covalently at the PUR surface by a two-step reaction, or by using amphiphilic polymers containing PEO as the hydrophilic part. The amphiphilic polymers consisted of one co
To compare the clinical and microbiological efficacy of azithromycin in curing chlamydial infections in women with that of lymecycline, and with a view of the possibility of minimizing the problem of compliance by means of single-dose administration, 146 women with culture-positive Chlamydia trachomatis infections were randomly assigned to treatment with a 1 g bolus dose of azithromycin or a 10-da
