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The complex dynamics of protein expression in plasma during hyperacute HIV-1 infection and its relation to acute retroviral syndrome, viral control, and disease progression are largely unknown. Here, we quantify 1293 blood plasma proteins from 157 longitudinally linked plasma samples collected before, during, and after hyperacute HIV-1 infection of 54 participants from four sub-Saharan African cou
The use of extracorporeal life support (ECLS) throughout the perioperative phase of lung transplantation requires nuanced planning and execution by an integrated team of multidisciplinary experts. To date, no multidisciplinary consensus document has examined the perioperative considerations of how to best manage these patients. To address this challenge, this perioperative utilization of ECLS in l
α-Synuclein (αSyn) is a neuronal protein predominantly found in the brain, whose native function seems to be associated with vesicle trafficking. While intrinsically disordered in solution, the first ca. 100 residues adopt an amphipathic α-helical structure when the protein adsorbs onto membranes. Additionally, the aggregation of αSyn into highly ordered β-sheet rich amyloid fibrils is associated
Lead-free metal halide perovskites are emerging as less-toxic alternatives to their lead-based counterparts. However, their applicability in optoelectronic devices is limited, and the charge transport dynamics remain poorly understood. Understanding photo-induced charge and structural dynamics is critical for unlocking the potential of these novel systems. In this work, ultrafast optical and Raman
Based on the DCV-C60 system of fullerene acceptor organic solar cell active materials, the charge transfer process of D-A type molecular materials under the action of an external electric field (Fext) was explored. Within the range of electric field application, the excited state characteristics exhibit certain regular changes. Based on reducing the excitation energy, the excitation mode shows a t
Isolation and characterization of circulating tumor cells (CTCs) present a noninvasive alternative to monitor disease progression in individual patients. However, the heterogeneous lineage specificity of CTCs makes it difficult to isolate and identify possible CTCs by a liquid biopsy. Better label-free methods for the isolation of viable CTCs are needed. Our solution is a combined approach that is
BACKGROUND/AIMS: Ventricular-arterial coupling (VAC) plays a crucial role in cardiovascular physiology, affecting cardiac function and arterial properties for optimal organ perfusion. Considering that diabetes mellitus (DM) is a known risk factor for incident heart disease and vascular damage, this study aims to investigate whether changes in VAC due to DM occur earlier, even before the onset of c
OBJECTIVE: To investigate associations between meniscal tear, bone marrow lesions (BMLs), and post-injury knee loading, with cartilage T2 relaxation times on knee magnetic resonance imaging (MRI) in the subacute phase following acute anterior cruciate ligament (ACL) injury.DESIGN: We studied both knees of 128 patients with ACL injury. The presence of meniscal tears and BMLs were determined on suba
Polarized fluorescence microscopy is a valuable tool for measuring molecular orientations in biological samples, but techniques for recovering three-dimensional orientations and positions of fluorescent ensembles are limited. We report a polarized dual-view light-sheet system for determining the diffraction-limited three-dimensional distribution of the orientations and positions of ensembles of fl
Among different lipid nanoparticle systems, sponge-phase nanoparticles (SPNPs) have recently attracted interest due to their ability to encapsulate large macromolecules along with demonstrated high interfacial activity. The potential application of SPNPs calls for investigations into how buffer conditions affect SPNP structure and interfacial activity. Herein, we systematically investigated how di
Over recent years many urban areas have experienced severe floods caused by extreme precipitation events. To avoid the devastating socioeconomic and environmental consequences of such floods, countries have issued laws or recommendations on flood risk assessment in urban planning. However, these laws or recommendations seldom include detailed requirements on input data quality or explicit instruct
In 2020, a case report described autologous transplantation of iPSC-derived dopamine (DA) neurons in a Parkinson's disease (PD) patient.1 The team now follows up with the pre-clinical safety and efficacy data of autologous iPSC-derived DA neurons, forming the basis for regulatory approval of a phase 1 clinical trial involving 8 patients.2
Histology is a long standing and well-established gold standard for pathological characterizations. In recent years however, synchrotron radiation-based micro-computed tomography (SRμCT) has become a tool for extending the imaging of two-dimensional thin sections into three-dimensional imaging of tissue blocks, enabling so-called virtual histology with arbitrary clipping planes, volumetric renderi
Understanding the perceptions of young people is a critical issue in the formulation and implementation of climate change mitigation strategies. Based on cognitive hierarchy theory, the present study aims to investigate the factors that motivate or hinder young people’s perception of forestation as a strategy to mitigate climate change in post-conflict Somalia. This study hypothesized a model in w
The emerging notion of hematopoietic stem and progenitor cells (HSPCs) as a low-primed cloud without sharply demarcated gene expression programs raises the question on how cellular-fate options emerge and at which stem-like stage lineage priming is initiated. Here, we investigate single-cell chromatin accessibility of Lineage-, cKit+, and Sca1+ (LSK) HSPCs spanning the early differentiation landsc
We have implemented and evaluated a secondary organic aerosol scheme within the chemistry transport model TM5-MP in this work. In earlier versions of TM5-MP the secondary organic aerosol (SOA) was emitted as Aitken-sized particle mass emulating the condensation. In the current scheme we simulate the formation of secondary organic aerosol from oxidation of isoprene and monoterpenes by ozone and hyd
RATIONALE: Vascular calcification is a prominent feature of late-stage diabetes, renal and cardiovascular disease (CVD), and has been linked to adverse events. Recent studies in patients reported that plasma levels of osteomodulin (OMD), a proteoglycan involved in bone mineralisation, associate with diabetes and CVD. We hypothesised that OMD could be implicated in these diseases via vascular calci
Purpose of Review: This article reviews controversial questions within the field of lung transplantation, with a focus on data generated within the last 3 years. We aim to summarize differing opinions on a selection of topics, including bridge-to-transplantation, intraoperative machine circulatory support, bronchial anastomosis, size mismatch, delayed chest closure, and ex vivo lung perfusion.Rece
To improve glycemic control in insulin-dependent diabetes, estimates of the glucose- lowering effect of insulin-the insulin action (IA)-and the dynamics thereof are essential. The current practice relies on generic estimates of the dynamic shape of the IA and rules-of-thumb to assess the total glucose-lowering effect. In this paper we present individualized estimates based on home-monitored data,
