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Heme proteins are central to metabolism and stress responses but remain challenging to express recombinantly due to cytotoxicity and folding constraints. Phytoglobins (Pgbs) exemplify these difficulties, as expression protocols often fail to translate across protein species. Here, we used a cell-free protein synthesis (CFPS) platform powered by digital microfluidics to screen expression determinan

District heating is critical for low-carbon urban energy systems, yet most networks remain centralized in both heat generation and data ownership, fossil-dependent, and poorly integrated with digital, customer-centric, and market-responsive solutions. While artificial intelligence (AI), the Internet of Things (IoT), and automation offer transformative opportunities, their adoption raises complex c

AbstractBackground: Identification of patients at risk and prevention of acute postoperative pain (APOP) are central to individualized anesthesia and analgesia. Venous cannulation pain (VCP) has shown promise as a predictor of APOP. In the PeriOPerative Individualization Trial (POPIT), VCP was evaluated as a pain-sensitivity stratification method to guide anesthesia and reduce postoperative pain.

At the heart of P. F. Strawson’s naturalistic approach to responsibility sits the Inescapability Claim. The main argument of this article is that the established interpretation of this claim is mistaken. According to, what I will call, the Standard Reading, it is the empirical claim that it is psychologically impossible for us to abandon our responsibility practices. Although widespread, this read

Context. Differentiating between in situ and accreted populations in the Milky Way halo is a challenging task. Various kinematic spaces are often used to identify distinct accreted populations from the in situ Milky Way halo. However, this approach has limitations, especially at low orbital energies. To overcome this ambiguity, elemental abundances are typically used to distinguish between populat

Ubiquitously used for monitoring pipelines and infrastructures, distributed fibre-optic acoustic sensing is becoming an emerging technology for earthquake monitoring thanks to the possibility to interrogate a large vast of distances with a dense number of sensing points in which an optical fiber cable can be retrofitted. Moreover, it can also be used as a tool by geophysicists for the characteriza

A fundamentally crucial suggestion to rectify the fits to the high-resolution X-ray photoelectron spectroscopy (XPS) raw data for the S 2p core level published by Dirin et al. (Nano Lett. 2023, 23 (5), 1914–1923) is presented herein.

Cognition in children with cerebral palsy (CP) is often affected. How cognition differs by functional level and subtype is not clear, and the effects of adapted response modes need investigation. In this study, the aims were to investigate how many psychologists reported that interpreters and/or augmentative and alternative communication (AAC) were used, how many assessments were adapted, if there

The faecal contamination of irrigation water threatens public health. Although safe practices can mitigate hygiene and food safety risks along the urban irrigated vegetable value chain, their adoption remains limited. A behaviour framework was combined with a participatory approach to explore how institutions influence stakeholders’ capability, opportunity and motivation to adopt safe practices in

Background and purpose: Neuroblastoma is the most common extracranial solid tumour in children. Radiotherapy is commonly part of the multimodal treatment for high-risk patients. The aim of this study is to analyse doses to organs at risk (OAR) in comparative proton and photon treatment plans for children treated for neuroblastoma and report side effects. Patient/material and methods: All children

Background Labels for neck pain after a motor vehicle crash (MVC) influenced recovery expectations and management preferences. Research is needed to understand why these expectations and preferences varied based on the label given. Aim To explore how people perceive different labels for neck pain after an MVC. Methods We performed a content analysis of qualitative data from a randomised controlled

Climate warming, nutrient loading, and pesticide pollution are major anthropogenic stressors affecting community dynamics and trophic interactions in aquatic ecosystems. However, the complex interplay among these stressors remains poorly understood as most studies focus on a single stressor and overlook the effects of temporal variations. To address this knowledge gap, we first conducted a long-te

Indigenous archaeology and archaeology of Indigeneity are paramount in the contemporary world. We certainly need more of it across the archaeological scale and across the archaeological globe. Archaeology’s unhealthy attachment to colonialism, colonial administration and imperialism keeps on affecting our discipline on all levels. It is therefore encouraging to read Felix Acuto’s call for an engag

Gold mining is widespread in the Colombian Amazon and negatively impacts biodiversity, climate, and social equity. While some claim that sustainable gold mining can address these issues, we have analyzed different narratives of sustainable gold mining, focusing on equity and power dimensions. Our research, conducted through participatory observation and interviews across scales between 2022 and 20

Analyzing kinetic experiments on protein aggregation using integrated rate laws has led to numerous advances in our understanding of the fundamental chemical mechanisms behind amyloidogenic disorders such as Alzheimer's and Parkinson's diseases. However, the description of biologically relevant processes may require rate equations that are too complex to solve using existing methods, hindering mec

The twin transition, which combines green and digital innovation in economic activities, is increasingly central to policy agendas and is also receiving growing attention in regional research. However, accurately mapping green, digital and twin (both green and digital) economic activities across regions remains challenging, particularly due to data constraints. In this study, we advance this resea

Objective: Severe obesity poses a major public health concern due to its links with cardiometabolic complications and mortality. Visceral adipose tissue (VAT) plays a key role in these processes through distinct molecular features. This study aimed to characterize the VAT proteome of individuals with severe obesity and investigate its association with serum metabolic biomarkers. Methods: A cross-s

Background: Fine-needle aspiration biopsy (FNAB) of lymph nodes is a widely used method for evaluating lymphadenopathy. FNAB offers general advantages of rapid turnaround time, low cost and minimal morbidity, and more specific advantages in various clinical situations, such as deeply located lymph nodes or patients with significant comorbidities. The FNAB sample can be utilized for a wide range of

Background: Low lung function has been consistently associated with increased cardiovascular disease (CVD) risk, with emerging evidence suggesting a potential causal relationship. However, underlying biological mechanisms remain unclear. Aim: To investigate relationships between CVD-associated plasma proteins and lung function. Methods: We analysed plasma protein profiles in two Swedish population

The meeting between public life in the city and our private bodily needs creates sensitive and complex architectural challenges, and public toilets are a clear example. They are often seen as merely practical and technical spaces, but they also reveal how a city thinks about accessibility, safety, and equality. How can these places meet basic needs while still feeling safe, clean, and welcoming? A