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Induction of a regulatory phenotype in human CD4(+) T cells by streptococcal M protein

Regulatory T cells (Tregs) participate in the control of the immune response. In the human system, an IL-10-secreting, T regulatory type 1 cell (Tr1)-like subset of Tregs can be induced by concurrent cross-linking of the TCR and CD46 on naive CD4(+) T cells. Because many viral and bacterial pathogens, including the major human pathogen Streptococcus pyogenes, bind to CD46, we asked whether this ba

Orbital and spin contributions to the g tensors in metal nanoparticles

We present a theoretical study of the mesoscopic fluctuations of g tensors in a metal nanoparticle. The calculations were performed using a semirealistic tight-binding model, which contains both spin and orbital contribution to the g tensors. The results depend on the product of the spin-orbit scattering time tau(so) and the mean-level spacing delta, but are otherwise weakly affected by the specif

Harmful azo colorants in leather. Determination based on their cleavage and extraction of corresponding carcinogenic aromatic amines using modern extraction techniques.

This study concerns the possibilities of using microwave-assisted extraction (MAE) or supercritical fluid extraction (SFE) for detection of harmful azo colorants in leather. After degreasing of the leather sample with SFE there follows a reductive cleavage of the azo colorants to their corresponding aromatic amines in the MAE or SFE equipment. The aromatic amines are subsequently extracted using e

HYDRO - A PROGRAM FOR PROTEIN HYDROPATHY PREDICTIONS

VAX/VMS programs for predicting protein hydropathy from amino acid sequence with several parameters are described. The hydropathy propensities of amino acids were normalised to make the different scales and predictions comparable. Another program package can be used to study similarity on the secondary structural level by comparing, for example, hydropathy predictions. These methods can provide va

Assessment of work postures and movements using a video-based observation method and direct technical measurements

The aim was to study postures and movements during repetitive work using video-based observations and direct technical measurements (inclinometers and goniometers). A total of 21 healthy women from a poultry processing plant volunteered. Neck flexion > 20 degrees was registered during 92% of the recorded time with the observation method, while the corresponding value measured with the inclinometer

Transient kinetics of ligand binding and role of the C-terminus in the dUTPase from equine infectious anemia virus

Transient kinetics of the equine infectious anemia virus deoxyuridine 5'-triphosphate nucleotide hydrolase were characterized by monitoring the fluorescence of the protein. Rate constants for the association and dissociation of substrate and inhibitors were determined and found to be consistent with a one-step mechanism for substrate binding. A C-terminal part of the enzyme presumed to be flexible

The cysteine protease inhibitors cystatins inhibit herpes simplex virus type 1-induced apoptosis and virus yield in HEp-2 cells

The role of cystatins in herpes simplex virus (HSV)-induced apoptosis and viral replication has been studied. Human epithelial (HEp-2) cells infected with wild-type HSV-1 (F), with a deletion virus lacking the anti-apoptotic gene Us3 (R7041) or with a deletion virus lacking the anti-apoptotic genes Us3 and ICP4 (d120) were treated with cystatin A, C or D. Cells and culture media were studied at di

Monte Carlo simulation of defect-free cross-linked polyelectrolyte gels

Model systems of cross-linked polyelectrolyte gels were investigated by means of Monte Carlo simulations. The model contained a charged defect-free th ee-dimensional network of a diamond-like topology and explicit counterions. Pressure vs volume relations and chain extensions were determined and compared to those of the corresponding polyelectrolyte solution. The structure of the gel was character

Routing in hybrid ad hoc networks using service points

In mobile ad hoc networks, nodes are often modeled as having the same capabilities. However, there are many cases in which certain nodes host special services to other nodes in the network. Examples of such services are Internet access, DNS proxy or AAA servers. We refer to these special nodes as netmarks. Because there is a high likelihood that netmarks will communicate with the rest of the netwo

Using simple mathematical functions to simulate pathological structures--input for digital mammography clinical trial.

In this study a set of structures has been simulated to represent a range of clinically relevant breast cancer mammographic lesions including solid tumours and microcalcifications. All structures have been created using simple random-based mathematical functions and have been inserted into a subset of digital mammography images at appropriate contrast levels into various regions of the breast, inc

Binding of SDS to Ethyl(hydroxyethyl)cellulose. Effect of Hydrophobic Modification of the Polymer

The binding of SDS to cellulose polymers in the semidilute concentration regime has been studied by means of NMR, ion-selective electrode, and a time-resolved fluorescence technique. Two polymers have been used, differing only in a low degree of hydrophobic modification of one of them. NMR self-diffusion and activity measurements show that the binding of SDS to the nonmodified polymer has a fairly