Calmodulin (CaM) is a ubiquitous Ca2+ sensing protein that binds to and modulates numerous target proteins and enzymes during cellular signaling processes. A large number of CaM-target complexes have been identified and structurally characterized, revealing a wide diversity of CaM-binding modes. A newly identified target is creatine kinase (CK), a central enzyme in cellular energy homeostasis. Thi

The most common and lethal bacterial pathogens have co-evolved with the host. Pathogens are the aggressors, and the host immune system is responsible for the defence. However, immune responses can also become destructive, and excessive innate immune activation is a major cause of infection-associated morbidity, exemplified by symptomatic urinary tract infections (UTIs), which are caused, in part,

Aquaporins (AQPs) are membrane channel proteins that facilitate the movement of water down osmotic gradients across biological membranes. This protocol allows measurements of AQP-mediated water transport across the plasma membrane of live mammalian cells. Calcein is a fluorescent dye that is quenched in a concentration-dependent manner. Therefore, on short timescales, its concentration-dependent f

The main premise of Self-Service Business Analytics (SSBA) is to make business employees autonomous during the data analytical process. To empower business employees, organizations are decentralizing their analytical capabilities through an SSBA approach. Yet, little is known about how employees integrate resources, such as, among others, personal competencies, environment resources including tech

Data philanthropy, which is firm donations of data, data scientists, and data technologies for social good, is a powerful new phenomenon that offers benefits to both donor firms and society. In this explorative research we unpack data philanthropy, providing definitions, and examples along with a theoretical perspective from corporate philanthropy and strategic management. We view data through a l

Among the major IT security challenges facing organizations is non-malicious employee behavior that nevertheless poses significant threats to an organization's IT security. Using a grounded theory methodology, this paper finds that organizational security behaviors are inherently related to employee assumptions regarding the importance of IT security policy compliance and regarding the reason why

Striving to promote the health and well-being of their workforce and decrease insurance expenditures, many organizations are incorporating wearable fitness trackers into their corporate wellness programs. Research suggests that these devices encourage individuals to make healthier choices; however, little is known about the drivers and impact of fitness trackers on employee health and wellbeing. W

In this introduction we provide an overview of research on gender issues in the rescue services in combination with introducing the different contributions in this book. The overview is primarily concerned with three national contexts, the US, the UK and Sweden, and we focus on the most prevalent themes in the literature as well as pointing out under-researched areas. The empirical contributions i

In this paper, super-resolution imaging is described and evaluated for x-ray tomography and is compared with standard tomography and upscaling during reconstruction. Blurring is minimized due to the negligible point spread of photon counting detectors and an electromagnetically movable micro-focus x-ray spot. Scans are acquired in high and lowmagnification geometry, where the latter is used to min

Objective To understand and report on the impact of the COVID-19 pandemic on the everyday lives of frail older persons living in nursing homes by exploring their experiences of how the pandemic-related restrictions had influenced them and in what way. Design Empirical qualitative interview study. Setting A publicly run nursing home in an urban area in Sweden in June 2020. The nursing home had visi

In vitro differentiation of human pluripotent stem cells (hPSCs) offers a genetically tractable system to examine the physiology and pathology of human tissue development and differentiation. We have used this approach to model the earliest stages of human B lineage development and characterize potential target cells for the in utero initiation of childhood B acute lymphoblastic leukemia. Herein,

Cooperative binding is a key feature of metabolic pathways, signaling, and transport processes. It provides tight regulation over a narrow concentration interval of a ligand, thus enabling switching to be triggered by small concentration variations. The data presented in this work reveal strong positive cooperativity of α-synuclein binding to phospholipid membranes. Fluorescence cross-correlation

Intermittent fasting may impart metabolic benefits independent of energy balance by initiating fasting-mediated mechanisms. This randomized controlled trial examined 24-hour fasting with 150% energy intake on alternate days for 3 weeks in lean, healthy individuals (0:150; n = 12). Control groups involved a matched degree of energy restriction applied continuously without fasting (75% energy intake

BACKGROUND: Radiostereometric analysis (RSA) is a highly accurate tool to detect implant migration and predict loosening following total knee arthroplasty (TKA). However, little is known about the predisposing risk factors for implant migration, nor which migration profile should be considered physiological (i.e., merely part of an implant-settling phase) and which should be considered pathologica

Background: Proton-pump inhibitors (PPIs) have been reported to increase the risk of community-associated Clostridium difficile infection (CDI), but the association remains disputed. Methods: A nationwide cohort study among adults in Denmark, 2010-2013, linking register data on C. difficile testing, filled prescriptions, and patient characteristics. All incident episodes of community-associated CD

Scientists are increasingly exploring on-site water systems to supplement conventional centralized water and wastewater infrastructure. While major technological advancements have been achieved, we still lack a systematic view on the non-technical, or institutional, elements that constitute important barriers to the uptake of on-site urban water management systems. This paper presents a conceptual

We show that algebraic proofs are hard to find: Given an unsatisfiable CNF formula F, it is NP-hard to find a refutation of F in the Nullstellensatz, Polynomial Calculus, or Sherali-Adams proof systems in time polynomial in the size of the shortest such refutation. Our work extends, and gives a simplified proof of, the recent breakthrough of Atserias and Müller (JACM 2020) that established an anal

The coexistence of Mott localized f states with wide conduction and valence bands in f-electron semiconductors results, quite generically, in a complex optical response with the nature of the absorption edge difficult to resolve both experimentally and theoretically. Here, we combine a dynamical mean-field theory approach to localized 4f shells with an improved description of band gaps by a semilo

Cooperative hunting involves individual predators relating in time and space to each other’s actions to more efficiently track down and catch prey. The evolution of advanced cognitive abilities and sociality in animals are strongly associated with cooperative hunting abilities as has been shown in lions, chimpanzees, and dolphins. Much less is known about cooperative hunting in seemingly unsocial